Anti-calf escherichia coli F41 pilus antigen hyperimmune egg yolk antibody IgY preparation method and application

A technology of Escherichia coli and egg yolk antibody, applied in the agricultural field, can solve the problems of insufficient purity, reduced effect of repeated use, and low coverage of Escherichia coli

Pending Publication Date: 2019-06-21
INNER MONGOLIA UNIV FOR THE NATITIES
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Problems solved by technology

The purity of anti-animal Escherichia coli hyperimmune egg yolk antibody IgY extraction is not high enough; Escherichia coli serotypes are complex and diverse, and the coverage of IgY for serotypes on pathogenic Escherichia coli is not high; animals after inoculation are prone to produce anti-IgY animal Escherichia coli Antibodies that are highly immune to egg yolk antibodies, the effect of repeated use is reduced

Method used

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  • Anti-calf escherichia coli F41 pilus antigen hyperimmune egg yolk antibody IgY preparation method and application
  • Anti-calf escherichia coli F41 pilus antigen hyperimmune egg yolk antibody IgY preparation method and application
  • Anti-calf escherichia coli F41 pilus antigen hyperimmune egg yolk antibody IgY preparation method and application

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Embodiment 1

[0057] (1) Isolation and identification of pathogenic bacteria; the specific steps are:

[0058] (a) Inoculate the collected calf diarrhea feces on ordinary agar medium, MacConkey medium and eosin methylene blue medium respectively, place them in a constant temperature incubator at 37°C for continuous culture for 24 hours, select a single colony for staining and microscopic examination, Then carry out purification culture;

[0059] (b) select the calf pathogenic Escherichia coli bacterial strain that isolates, inoculate respectively nutrient broth substratum culture, the preparation bacteria content is 10 9 CFU / mL suspension was inoculated into the mice intraperitoneally, each mouse was inoculated with 0.2mL, a total of 5 mice, and a group of blank control mice were inoculated with sterile nutrient broth, each mouse was inoculated with 0.2mL, and a total of 5 mice were inoculated. Five mice were isolated and observed for 5 days after inoculation, and the death of the mice was...

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Abstract

The invention relates to the technical field of agriculture, in particular to an anti-calf escherichia coli F41 pilus antigen hyperimmune egg yolk antibody IgY preparation method and application. Themethod includes steps: (1) pathogenic bacterium separation and identification; (2) pilus gene identification and screening; (3) pilus antigen extraction and purification; (4) egg yolk sterile separation; (5) antibody purification. Clear thinking is realized, by adoption of calf escherichia coli F41 pilus as an antigen for immunization of egg-laying hens, the egg-laying hens generate an anti-pilusantibody, hyperimmune eggs are collected, egg yolks are separated, and an antibody extracted from the egg yolks is a hyperimmune egg yolk antibody (IgY), and the hyperimmune egg yolk antibody is applied to treatment and prevention of colibacillosis of calves.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a preparation method and application of an anti-calf Escherichia coli F41 pili antigen hyperimmune egg yolk antibody IgY. Background technique [0002] Calf Escherichia coli is a common bacterial infectious disease, and calves have a high mortality rate after the onset. There are many serotypes of pathogenic Escherichia coli, which brings difficulties to the prevention of this disease. Generally, antibiotics are selected for treatment, but antibiotics often develop drug resistance during treatment, or cause the beneficial bacteria in the intestinal tract of calves to degenerate. damage and delay treatment. In particular, the promulgation and implementation of the anti-antibiotic ban has reduced the number of antibiotics that can be used clinically, which has brought difficulties to the treatment of calf colibacillosis. [0003] Animals are generally stimulated by pathogen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/12C07K16/02C12N1/20C12N1/02C12N15/10C12N15/66C07K14/245A61K39/40A61P31/04A61P1/12C12R1/19
CPCY02A50/30
Inventor 刘锴马德慧白浩丞石芸史量全王永强尹永勤李天旭郭双张荣荣李钗周虹
Owner INNER MONGOLIA UNIV FOR THE NATITIES
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