Liquid-mass analysis method for detecting the content of catecholamine metabolites in blood

Abstract

The liquid-mass analysis method for detecting the content of catecholamine metabolites in blood comprises steps of calibrating a standard solution by a high-performance liquid chromatography triple quadrupole tandem mass spectrometer; fitting a standard curve equation: y1=a*x1+b, y2= c*x2+d and y3=e*x3+f; after the blood to be detected is treated, detecting a sample to be detected also by the high-performance liquid chromatography triple quadrupole tandem mass spectrometer to obtain the values y1, y2 and y3 of the blood to be detected; substituting the values y1, y2 and y3 into the standard curve equation, and calculating the relative concentrations x1, x2 and x3 of three substances of the catecholamine in the blood to be detected, wherein the concentration of an internal standard substance working fluid is known, and accordingly the concentrations of the catecholamine metabolites in the blood to be detected in the sample is calculated. The plasma is directly injected after being treated by off-line SPE and the method is simple and rapid in detection process, and high in detection sensitivity, and contributes to the determination of the content of catecholamine in a patient.

Description

Technical field [0001] The invention relates to the technical field of clinical content concentration monitoring of neurological substances, and in particular to a method for detecting the content of catecholamine metabolites of neurological substances in blood. Background technique [0002] Pheochromocytoma is a tumor that originates in the adrenal medulla, sympathetic ganglia, and parasympathetic ganglia that can secrete excessive catecholamines and can appear in the adrenal gland, bladder, abdominal aorta, retroperitoneum, heart and other chromaffin tissues. Because the tumor tissue can secrete a large amount of catecholamines paroxysmal or persistently, clinical symptoms of paroxysmal or persistent hypertension often appear, and the manifestations are diversified. The symptoms of the cardiovascular system are mainly the symptoms of other metabolic disorders. Pheochromocytoma is dangerous and changes drastically. If it is not treated in time, it can easily cause cardiovascular...

AI Technical Summary

Problems solved by technology

[0004] The chemical structure of catecholamine metabolites is characterized by a dihydroxybenzene nucleus and a side chain with an amino group. The content of catecholamines in biological samples is low, and the activity of catecholamine metabolites is extremely poor and easily oxidized. Coupled with the simultaneous presence of various endogenous chemical interferents of metabolites with similar structures and / or with the same chemical group in biological samples, it has led to difficulties in the precise determination of the concentration of catecholamine metabolites in biological samples

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