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Application of basic chitinase from vibrio in fungus dyeing

A technology of chitinase and Vibrio, which is applied in the field of pathogenic bacteria biological inspection, can solve the problems of fast fluorescence decay, lack of specificity, interference, etc.

Active Publication Date: 2019-06-28
MERLIN BIOMEDICAL (XIAMEN) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is still not specific, susceptible to interference from staining substrate impurities, and the fluorescence fades quickly

Method used

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  • Application of basic chitinase from vibrio in fungus dyeing
  • Application of basic chitinase from vibrio in fungus dyeing
  • Application of basic chitinase from vibrio in fungus dyeing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Fluorescence staining (one-step method) of embodiment 1 fungal culture

[0050] Scrape the cultured Candida with a sterile scalpel blade, apply it on an anti-shedding glass slide, add a drop of the above staining solution, let it stand at room temperature for 2-5 minutes, and then observe it under a fluorescent microscope. The result shows blue fluorescence, as shown in Fig. figure 1 shown.

Embodiment 2

[0051] Fluorescent staining (two-step method) of embodiment 2 nail scraps

[0052] Samples 1 and 2 of the fungal dermatitis were scraped with a sterile scalpel blade, and each sample was smeared on 3 anti-off slides, and divided into 3 groups, namely, group 1 to group 3, and each group included a sample 1 and a specimen 2. Add a drop of 10% potassium hydroxide dropwise to the first group of specimens, let stand at room temperature for 2 to 5 minutes to dissolve cutin, rinse the glass slide with purified water, add dropwise a drop of staining solution (except that it does not contain KOH, the rest of the formula is the same as in Example 1 staining solution), let stand at room temperature for 2 to 5 minutes, and then observe under a fluorescence microscope, the results show blue fluorescence, such as figure 2 and image 3 shown, where figure 2 Group 1 for specimen 1, image 3 Group 1 for specimen 2.

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Abstract

The invention discloses application of basic chitinase from vibrio in fungus dyeing. The basic chitinase from the vibrio is applied to dye fungi, and can targetedly and specifically bind to chitin ofthe fungus cell wall, observation is conducted under a fluorescence microscope, ultraviolet light of 340-400 nm is used for activating, and the fungi can show blue or yellow-green color.

Description

technical field [0001] The invention belongs to the technical field of biological detection of pathogenic bacteria, and in particular relates to the application of alkaline chitinase from Vibrio in fungal staining. Background technique [0002] Fungi can easily cause fungal skin diseases. When fungi invade the human body, doctors will classify them according to the parts they invade the human body. Therefore, fungi can be divided into superficial fungi and deep fungi. Superficial fungi (ringworm) only invade skin, hair and nails, while deep fungi can invade human skin, mucous membranes, deep tissues and internal organs, and even cause severe sepsis, endocarditis, meningitis and other fatal diseases . [0003] In modern medical clinical testing, the diagnosis of superficial fungal infections mainly relies on direct microscopic examination and culture of fungi from clinical samples. In the diagnosis of traditional fungal infection, KOH wet film microscopy is the simplest an...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 沈义峰陈丽宏
Owner MERLIN BIOMEDICAL (XIAMEN) CO LTD
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