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Gelatin polymer derived from natural sources of cold-adapted marine species and uses thereof

A natural source, polymer technology, applied in the preparation, application, and peptide source of animal glue or gelatin, which can solve problems such as poor rheological properties

Inactive Publication Date: 2019-06-28
UNIV DE LOS ANDES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In particular, fish gelatin has been proposed as an alternative, but an important challenge to overcome is its poor rheological properties

Method used

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  • Gelatin polymer derived from natural sources of cold-adapted marine species and uses thereof
  • Gelatin polymer derived from natural sources of cold-adapted marine species and uses thereof
  • Gelatin polymer derived from natural sources of cold-adapted marine species and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0197] Example 1. Preparation of methacryloyl salmon gelatin solution.

[0198] - Extraction of salmon gelatin:

[0199] 1. Remove any remaining flesh and scales from the salmon skin.

[0200] 2. Cut the skin into small squares (3-5cm / side)

[0201] 3. Add 6 mL of 0.1 M NaOH per gram of skin and maintain constant stirring (9000 rpm) for 1 hour at 10°C. →6mL / g*500g=3L (measured pH=13)

[0202] 4. Remove the solution and wash with dH 2 O Wash the skin by filtering and soaking.

[0203] 5. Add 6 mL of 0.1M NaOH per gram of skin and keep stirring at 10°C for 1 hour. → 3L (measured pH = 13)

[0204] 6. Remove the solution and filter through dHO 2 O washes the skin with dH 2 O Rinse and soak the filtered skin.

[0205] NOTE: Too much washing at this step may affect the pH of the acidic solution in step 7, which may lead to higher acidity and thus induce partial extraction of gelatin which needs to be avoided at this stage.

[0206] 7. Then, add 6 mL of 0.05M CH per gram ...

Embodiment 2

[0217] Example 2. Preparation of methacryloyl bovine gelatin solution.

[0218] Similar to Example 1, after mixing with methacrylic anhydride and reacting with amino groups (mainly lysine) in gelatin solution, as previously described (Nichol et al. 2010; Van Den Bulcke et al. 2000). , Synthesis of methacryloyl bovine gelatin. Briefly, bovine gelatin (Bloom 220, Rousselot, The Netherlands) was dissolved in PBS 1X (pH 7.4) at 60°C to a final concentration of 10% (w / v). After complete dissolution, while still stirring, methacrylic anhydride (276685, Sigma, USA) was slowly added to a final concentration of 8% (v / v). Different levels of methacryloyl functionalization require different concentrations of methacrylic anhydride. After 3 hours of reaction, a 5X dilution was made in PBS 1X, and the reacted gelatin was dialyzed against deionized water at 40°C for 1 week. Fresh deionized water was changed daily to remove all unreacted methacrylic anhydride during dialysis. Finally, the...

Embodiment 3

[0219] Example 3. Preparation of non-methacryloyl salmon gelatin solution.

[0220] Salmon gelatin was extracted according to the instructions in Example 1, and non-methacryl salmon was usually prepared by dissolving the obtained gelatin in PBS 1X (pH 7.4) at 60°C to a final concentration of 10% (w / v) gelatin. Solutions of different concentrations of gelatin required dissolving different amounts of ground gelatin in PBS 1X.

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Abstract

The present invention refers to a composition comprising a solution which in turn comprises an amino acidic chain gelatin polymer derived from natural sources of cold-adapted marine species, at a concentration from 1% to 20% (w / v), which optionally further comprises a polymerizing initiator such as a photoinitiator, and is chemically functionalized to become reactive to polymerization or crosslinking in presence of free radicals. This composition is especially useful for 3D printing, extrusion systems (additive fabrication), spray systems, casting, micro-and nano fibers fabrication systems (electrospinning, solution blow spinning) or microfluidics.

Description

technical field [0001] The present invention relates to the biomedical field and the digestive industry, and in particular to compositions comprising a solution of gelatin polymers containing amino acid chains in a concentration of at least 20% (w / v) in turn of 1% (w / v), said solution being other than Necessarily also containing a photoinitiator or any other type of free radical derived initiator, the amino acid chain gelatin polymer is derived from a natural source of cold adapted marine species and is chemically functionalized to become reactive so as to react with free radicals Polymerization or crosslinking takes place in the presence. This composition is particularly suitable for novel biomanufacturing techniques used in the fields of biomedicine and digestion. Background technique [0002] In the field of biomedicine, novel biofabrication techniques allow the preparation of biomaterials such as scaffolds, beads, engineered tissues, devices and microdevices for therape...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H1/00C07K14/78C07K14/46C08J3/24
CPCC07K14/78C07K14/461C08H1/00B33Y70/00C08L89/00C08L71/02C09H7/00A61L27/222A23P20/10C08L89/04C08J3/075C08J2389/06C08K5/101C08L89/06C08L2312/00
Inventor 哈维尔·恩里昂·卡塞雷斯保罗·迪亚兹-卡尔德隆亚历山大·佐帕胡安·巴勃罗·阿塞韦多·考克斯
Owner UNIV DE LOS ANDES
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