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Application of three components of scutellaria baicalensis to synergistically improving cell proliferation enhancement of FGF2 (fibroblast growth factors 2)

A technology of FGF2- and baicalin, applied in the field of molecular biology, to achieve the effect of clear target

Active Publication Date: 2019-07-05
YANBIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of the synergistic effect between the components of traditional Chinese medicine and the synergistic effect of the combined administration of traditional Chinese medicine and protein drugs, and provide the application of three components of Scutellaria baicalensis synergistically enhancing FGF2 to promote cell proliferation

Method used

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  • Application of three components of scutellaria baicalensis to synergistically improving cell proliferation enhancement of FGF2 (fibroblast growth factors 2)
  • Application of three components of scutellaria baicalensis to synergistically improving cell proliferation enhancement of FGF2 (fibroblast growth factors 2)
  • Application of three components of scutellaria baicalensis to synergistically improving cell proliferation enhancement of FGF2 (fibroblast growth factors 2)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of Radix Scutellariae Extract

[0029] Purchase dried scutellaria baicalensis slices from pharmacies, remove impurities, grind the dried scutellaria baicalensis into a uniform powder, weigh 2 g of the powder and add 20 mL of methanol for ultrasonic extraction, extract 3 times, each time for 20 min, filter and combine the extracts, pass through 0.22 μm The filter membrane was blown with nitrogen, concentrated and dried, redissolved in ultrapure water, passed through a 0.22 μm filter membrane, and stored at 4°C until use.

Embodiment 2

[0030] Example 2 Screening the components that bind to FGF2 in the extract of Scutellaria baicalensis

[0031] 1. Preparation of FGF2-Scutellaria baicalensis active ingredient complex

[0032] FGF2 lyophilized powder was dissolved in 10 mM pH6.8 ammonium acetate buffer to obtain FGF2 ammonium acetate solution; according to the ratio of FGF2 ammonium acetate solution: Scutellaria baicalensis extract (v:v) = 1:1, mix to form FGF2-Scutellaria baicalensis extract liquid mixture; incubate at 37°C for 30 minutes to make the active ingredient in the Scutellaria baicalensis extract combine with FGF2 to form a complex of FGF2-Scutellaria baicalensis active ingredient;

[0033] 2. Multi-chamber electrophoresis separation

[0034] The hatched FGF2-scutellaria baicalensis extract mixture is used as the sample solution, and the scutellaria baicalensis extract is used as the control solution, which are respectively the experimental group and the control group; multi-chamber electrophoresis...

Embodiment 3

[0048] Example 3 Qualitative analysis of active components of Scutellaria baicalensis combined with FGF2

[0049] 1. The solution in the receiving chamber after the dissociation of Example 2 is subjected to HPLC high performance liquid chromatography-tandem mass spectrometry and nuclear magnetic resonance spectrum analysis;

[0050] Chromatographic conditions:

[0051] Chromatographic column: C18 (4.6 mm×150 mm, 5 μm)

[0052] Mobile phase: 0.1% formic acid aqueous solution (A), 0.1% formic acid acetonitrile solution (B)

[0053] Injection volume: 5μL

[0054] Flow rate: 0.5 mL min -1

[0055] Gradient elution procedure:

[0056] Time (min) Mobile phase B (%)

[0057] 0 0

[0058] 7 0

[0059] 10 25

[0060] 20 40

[0061] 30 65

[0062] 40 80

[0063] Mass Spectrometry Conditions:

[0064] Electrospray ion source, negative ion mode, using full scan mode; spray gas pressure: 30 psi; dry gas (N 2 ) flow rate: 13.0 L / min, drying gas temperature: 300°C; capillary vo...

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Abstract

The invention discloses scutellaria baicalensis extract-FGF2 compounds. The scutellaria baicalensis extract-FGF2 compounds are obtained by together incubating scutellaria baicalensis extracts and FGF2, wherein the scutellaria baicalensis extracts are baicalin, oroxylin A-7-0-beta-D-glucuronide or wogonoside, the obtained scutellaria baicalensis extract-FGF2 compound are FGF2-baicalin compounds, FGF2-oroxylin A-7-0-beta-D-glucuronide compounds or FGF2-wogonoside compounds; applied incubation includes dissolving the scutellaria baicalensis extracts with methyl alcohol of three times by weight; dissolving FGF2 target proteins into PBS (phosphate buffer saline) solution; mixing the FGF2 PBS solution with the scutellaria baicalensis methyl alcohol solution; performing incubation at 36-38 DEG Cfor 25-35 min and then freeze-drying. The FGF2-baicalin compounds, the FGF2-oroxylin A-7-0-beta-D-glucuronide compounds and / or the FGF2-wogonoside compounds are applied to cell proliferation enhancement.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and specifically relates to the use of three components of Scutellaria baicalensis synergistically enhancing FGF2 to promote cell proliferation. Background technique [0002] The ingredients of traditional Chinese medicine are complex and diverse, and the development of feasible and rapid screening and identification of active ingredients of modern Chinese medicine is the key to the modern research and development of Chinese medicine. The screening of bioactive components is an important content in the field of traditional Chinese medicine research. Traditional chemical separation, structure identification, activity screening mode, and activity-oriented chemical separation have many shortcomings such as unclear goals, cumbersome operations, heavy workload, long cycle, and easy loss of active components during the separation process. Modern pharmacological studies have shown that the af...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/18A61K31/7048A61P43/00
CPCA61K38/1825A61K31/7048A61P43/00A61K2300/00
Inventor 蒋世翠金春燕李东浩郭建鹏
Owner YANBIAN UNIV
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