Protein PwRBP1 related to stress tolerance of plants, and encoding gene and application thereof

A protein and plant technology, applied in the direction of plant gene improvement, application, plant peptides, etc., can solve the problems of few reports on RBP gene function.

Active Publication Date: 2019-07-05
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

RNA binding proteins can play an important role in the growth and development of organisms, in response to low temperature, flooding, drought, high salinity, high temperature and other stresses. Currently, RBP genes in model plants such as Arabidopsis and rice are associated with plant stress resistance Related studies have made important progress, but there are few reports on the function of RBP genes in woody plants

Method used

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  • Protein PwRBP1 related to stress tolerance of plants, and encoding gene and application thereof
  • Protein PwRBP1 related to stress tolerance of plants, and encoding gene and application thereof
  • Protein PwRBP1 related to stress tolerance of plants, and encoding gene and application thereof

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Experimental program
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Effect test

Embodiment 1

[0078] Embodiment 1, the acquisition of PwRBP1 and its coding gene

[0079] 1. Acquisition of PwRBP1 and its coding gene

[0080] In this paper, using the cDNA library of Cinnamon cDNA constructed by the Gateway method completed by Yingwei Jieji (Shanghai) Co., Ltd. as a template, 5'-CTGATGAATATCGACTTTGGAAAGTC-3' and 5'-ATGATGCAACCCACAGCAGG-3' were used for PCR amplification to obtain positive cDNA sequence, and sequenced.

[0081] The sequencing results show that the cDNA nucleotide sequence is as shown in SEQ ID NO.1 in the sequence listing, consisting of 1293 nucleotides, the gene is named PwRBP1, the protein encoded by PwRBP1 is named PwRBP1, and its amino acid sequence is the sequence listing Among them, SEQ ID NO.2 consists of 431 amino acids.

[0082] The above cDNA can be obtained by artificial synthesis.

[0083] 2. Fluorescent quantitative PCR analysis of tissue expression of PwRBP1 gene

[0084] RNA from pollen, roots, stems, needles, and seeds of T. chinensis w...

Embodiment 2

[0086] Embodiment 2, the application of PwRBP1 gene in improving plant stress tolerance

[0087] 1. Construction of transgenic PwRBP1 Arabidopsis

[0088] 1. Acquisition of PwRBP1 gene

[0089] Prepare the following amplification primer pairs:

[0090]Primer 1: 5'-ATGATGCAACCCACAGCAGG-3';

[0091] Primer 2: 5'-CTGATGAATATCGACTTTGGAAAGTC-3'.

[0092] Using the cDNA of P. chinensis as a template, the above primers 1 and 2 were used for PCR amplification to obtain a PCR product with a size of 1293 bp, which was sequenced after being connected with the pEASY-T1 vector.

[0093] Sequencing results show that the nucleotide sequence of the PCR product is SEQ ID NO.1 in the sequence listing, which can encode the protein PwRBP1 shown in SEQ ID NO.2 in the sequence listing.

[0094] 2. Acquisition of recombinant expression vector

[0095] Prepare the following primer pairs:

[0096] Primer 1: 5'-CGCGGATCCATGATGCAACCCACAGCAGG-3';

[0097] Primer 2: 5'-TCCCCCGGGCTGATGAATATCGACTTTGG...

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Abstract

The invention discloses an RNA binding protein PwRBP1 related to stress tolerance of plants, and an encoding gene and application thereof. The PwRBP1 encoding gene discovered in woody plants is introduced into arabidopsis thaliana to obtain PwRBP1 transgenic arabidopsis thaliana plants. Experiments prove that the PwRBP1 transgenic arabidopsis thaliana plants have higher drought tolerance and salttolerance than receptor plants, indicating the stress tolerance of the PwRBP1 and the encoding gene thereof, so that the PwRBP1 and the encoding gene thereof are suitable for popularization and application.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to an RNA binding protein PwRBP1 related to plant stress tolerance, its coding gene and application. Background technique [0002] Drought stress and salt stress are two main abiotic environmental stresses, which will cause plants to produce such as osmotic damage, ion damage, accumulation of active oxygen and toxic substances, etc., which will seriously affect plant seed germination, photosynthesis, plant growth and development. and other physiological and growth processes. In the long-term evolution process, plants will form a series of effective mechanisms, including regulating the expression of stress-related genes to cope with the adverse external environment. [0003] RBP protein is an RNA-binding protein, which has the ability to bind RNA in eukaryotes. RNA-binding proteins play a key role in the process of post-transcriptional regulation. They can coordinate the tran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82C12N5/10A01H5/00
CPCC07K14/415C12N15/8273
Inventor 张凌云崔潇月张鹤华
Owner BEIJING FORESTRY UNIVERSITY
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