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Method for effectively and continuously extracting selenium active component in moringa seeds

An extraction method and a technology of active ingredients, which are applied in the extraction field of effective maintenance of selenium active ingredients in Moringa oleifera seeds, can solve the problems of not knowing how to maintain selenium, not involving the selenium content in Moringa oleifera seed polysaccharides, etc., and achieve the effect of less loss

Inactive Publication Date: 2019-07-12
BEIBU GULF UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Yet above-mentioned prior art all does not relate to the mensuration of the selenium content in the obtained Moringa seed polysaccharide, and it is not clear whether the obtained Moringa seed polysaccharide effectively keeps the selenium in the Moringa seed

Method used

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  • Method for effectively and continuously extracting selenium active component in moringa seeds
  • Method for effectively and continuously extracting selenium active component in moringa seeds
  • Method for effectively and continuously extracting selenium active component in moringa seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] 1) The Moringa oleifera seeds were dried at a constant temperature of 40°C, crushed, passed through a 100-mesh molecular sieve, and the obtained Moringa oleifera seed powder and petroleum ether were soaked at a solid-liquid ratio of 1g:3mL for 7 days to degrease, and the petroleum ether was recovered by distillation under reduced pressure, and the residue was placed in After volatilizing the solvent in a ventilated place, the defatted Moringa seed powder is obtained;

[0089] 2) Put 2g of degreased Moringa oleifera seed powder in a flask, add 80v / v% ethanol for desorption at a solid-liquid ratio of 5.0mL / g for 25min, then immediately add 50mL of 80°C hot water for extraction for 6min, suction filter, and repeat the above for the filter residue Extract once, combine filtrate, promptly obtain 104.5mL Moringa seed polysaccharide solution; Measure (below the same) selenium content in Moringa seed polysaccharide solution by the method described in aforementioned 1.2.3, be 3.0...

Embodiment 2

[0098] 1) The Moringa oleifera seeds were dried at a constant temperature of 40°C, crushed, passed through a 100-mesh molecular sieve, and the obtained Moringa oleifera seed powder and petroleum ether were soaked at a solid-liquid ratio of 1g:5mL for 5 days to degrease, and the petroleum ether was recovered by distillation under reduced pressure. After volatilizing the solvent in a ventilated place, the defatted Moringa seed powder is obtained;

[0099] 2) Put 2g of degreased Moringa oleifera seed powder in a flask, add 80v / v% ethanol for desorption at a solid-liquid ratio of 6.0mL / g for 30min, then immediately add 50mL of 80°C hot water for extraction for 7min, suction filter, and repeat the above for the filter residue Extract once, combine filtrate, promptly obtain 106mL Moringa seed polysaccharide solution;

[0100] 3) The obtained Moringa oleifera seed polysaccharide solution is concentrated to a paste, then Sevage reagent is added thereto, fully oscillated, stirred for 3...

Embodiment 3

[0102] 1) The Moringa oleifera seeds were dried at a constant temperature of 40°C, crushed, passed through a 100-mesh molecular sieve, and the obtained Moringa oleifera seed powder and petroleum ether were soaked at a solid-liquid ratio of 1g:4mL for 6 days to degrease, and the petroleum ether was recovered by distillation under reduced pressure. After volatilizing the solvent in a ventilated place, the defatted Moringa seed powder is obtained;

[0103] 2) Put 2g of degreased Moringa oleifera seed powder in a flask, add 80v / v% ethanol for desorption at a solid-liquid ratio of 5.0mL / g for 25min, then immediately add 40mL of 80°C hot water for extraction for 7min, suction filter, and repeat the above for the filter residue Extract once, combine filtrate, promptly obtain 85.5mL Moringa oleifera seed polysaccharide solution;

[0104] 3) The obtained Moringa oleifera seed polysaccharide solution is concentrated to a paste, then Sevage reagent is added thereto, fully oscillated, sti...

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Abstract

The invention discloses a method for effectively and continuously extracting a selenium active component in moringa seeds. The method specifically comprises the following steps: taking 80v / v percent alcohol as a desorption reagent to perform desorption on moringa seed powder, then adding 80 DEG C hot water to perform extraction, and collecting an extracting solution; performing deproteinization and centrifugation on the extracting solution, taking supernate, performing alcohol precipitation on the supernate, and collecting sediment, so as to obtain moringa seed selenium polysaccharide; ensuring that the ratio of the volume mL of the desorption reagent and the mass g of defatted moringa seed powder is greater than or equal to 5 and the desorption time is greater than or equal to 25min during desorption; ensuring that the extraction time for each time is greater than or equal to 6min. The method is simple in technology and short in extraction time, ensures that selenium and polysaccharide in moringa seeds can be sufficiently extracted, and ensures that the selenium active component in the moringa seeds is effectively kept in the extracting solution.

Description

technical field [0001] The invention relates to the extraction of active substances in moringa seeds, in particular to an extraction method for effectively maintaining selenium active components in moringa seeds. Background technique [0002] Moringa (Moringa oleifera Lam.), native to India, Kenya and Ethiopia, has been widely planted in tropical and subtropical regions such as Asia and Africa, and is planted in Yunnan, Hainan, Guangdong and Guangxi in my country. The roots, stems, leaves, flowers, and seeds of Moringa are rich in selenium, calcium, magnesium, potassium and other minerals needed by the human body. They are also rich in amino acids, vitamin A, vitamin C, and vitamin B group elements. They are high-quality raw materials for health products. In November 2012, the Ministry of Health of my country approved Moringa leaf as a new resource food. Moringa contains flavonoids, saponins, polysaccharides, alkaloids, polypeptides, amino acids and unsaturated fatty acids...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 郑韵英尹艳镇钟书明张瑞瑞梁兴唐刘子杰焦淑菲刘敏卿垂军宾洁龚丽华
Owner BEIBU GULF UNIV
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