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Environmental pollutant detection method based on three-dimensional micro-nano structure-enhanced Raman spectroscopy

A technology of environmental pollutants and micro-nano structures, applied in the field of environmental pollutant detection based on three-dimensional micro-nano structure-enhanced Raman spectroscopy, can solve the problems of complex preparation process, high detection cost, expensive equipment, etc., and achieve simple operation and high sensitivity High and low cost effect

Active Publication Date: 2021-11-23
CHONGQING ENVIRONMENT & SANITATION GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are expensive in equipment, complicated in preparation process, time-consuming, and high in detection cost.
[0004] Therefore, it is one of the key problems to solve the time-consuming microfluidic SERS test and reduce the cost of equipment to develop a convenient method for SERS substrate to improve the SERS signal, so as to accurately analyze the Raman spectra of different samples to be tested.

Method used

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  • Environmental pollutant detection method based on three-dimensional micro-nano structure-enhanced Raman spectroscopy
  • Environmental pollutant detection method based on three-dimensional micro-nano structure-enhanced Raman spectroscopy
  • Environmental pollutant detection method based on three-dimensional micro-nano structure-enhanced Raman spectroscopy

Examples

Experimental program
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Effect test

Embodiment 1

[0029] The Raman spectrum of methylene blue (MB) is detected, and the specific detection is as follows:

[0030] (1) First, soak the copper particles in a dilute sulfuric acid solution with a mass fraction of 2.0% for 10 minutes, and pass the soaked copper particles into the PDMS microfluidic pipeline. The amount of copper particles introduced is 1 / 3- 1 / 2, and inject 1mM silver nitrate solution at a rate of 10μL / min, the reaction time is 10min, and finally wash the copper particles with ethanol until the liquid on the surface of the copper particles is washed away, and the three-dimensional micro-nano structure SERS substrate is obtained;

[0031] (2) Prepare MB to a concentration of 10 -4 M, 10 -5 M, 10 -6 M, 10 -7 M and 10 -8 M aqueous solution, and pass the above solution into the PDMS micro-flow pipeline at a rate of 10 μL / min, and the passing time is 10 minutes; Concentration sequential access;

[0032] (3) Place the microfluidic system on the stage of the confocal ...

Embodiment 2

[0034] The Raman spectrum of urea is detected, and the specific detection is as follows:

[0035] (1) First, the copper particles are soaked in a dilute sulfuric acid solution with a mass fraction of 1.0% for 10 minutes, and the soaked copper particles are passed into the PDMS microfluidic pipeline, and the amount of copper particles introduced is 1 / 3- 1 / 2, and inject 2mM silver nitrate solution at a rate of 10μL / min, the reaction time is 5min, and finally wash the copper particles with ethanol until the liquid on the surface of the copper particles is washed away, and the three-dimensional micro-nano structure SERS substrate is obtained;

[0036] (2) Take a certain amount of deionized water and heat it to 90°C, and add urea to the deionized water to prepare a concentration of 10 - 4 M, 10 -5 M, 10 -6 M, 10 -7 M and 10 -8 M aqueous solution, and pass the above solution into the PDMS micro-flow pipeline at a rate of 10 μL / min, and the passing time is 10 minutes; Concentra...

Embodiment 3

[0039] The Raman spectrum of melamine is detected, and the specific detection is as follows:

[0040] (1) First, the copper particles are soaked in dilute sulfuric acid solution with a mass fraction of 3.0% for 10 minutes, and the soaked copper particles are passed into the PDMS microfluidic pipeline, and the amount of copper particles introduced is 1 / 3- 1 / 2, and inject a 4mM silver nitrate solution at a rate of 10μL / min, and the reaction time is 150min. Finally, wash the copper particles with ethanol until the liquid on the surface of the copper particles is washed away, and the three-dimensional micro-nano structure SERS substrate is obtained;

[0041] (2) Take a certain amount of deionized water and heat it to 90°C, and add melamine to the deionized water to prepare a concentration of 10 -5 M, 10 -6 M, 10 -7 M and 10 -8 M aqueous solution, and pass the above solution into the PDMS micro-flow pipeline at a rate of 10 μL / min, and the passing time is 10 minutes; Concentrat...

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Abstract

The invention provides a method for detecting environmental pollutants based on three-dimensional micro-nano structure-enhanced Raman spectroscopy, comprising the following steps: (1) first soaking copper particles in dilute sulfuric acid solution for 5-15min, and passing the soaked copper particles into Into the PDMS microfluidic pipeline, and inject the silver nitrate solution at a rate of 10 μL / min, the reaction time is 5-15min, and finally wash the copper particles with ethanol until the liquid on the surface of the copper particles is washed away, and the three-dimensional micro-nano structure SERS substrate is obtained; (2) Prepare the sample to be tested into a solution, and pass it into the PDMS microfluidic pipeline at a rate of 10 μL / min; (3) Place the microfluidic system on the stage of the confocal Raman detection platform, adjust the microscope The distance between the objective lens and the sample, focus the laser focus on the sample, and collect the Raman spectrum signal of the sample to be tested. The detection method has high sensitivity, is portable and miniaturized, and the prepared SERS substrate has filterability and recognition for cancer cells.

Description

technical field [0001] The invention belongs to the technical field of surface-enhanced Raman spectroscopy, and in particular relates to an environmental pollutant detection method based on three-dimensional micro-nano structure-enhanced Raman spectroscopy. Background technique [0002] With the deepening of research, the application of surface-enhanced Raman spectroscopy (SERS) has been extended to various fields. Among these applications, SERS testing combined with microfluidic devices has shown some clear advantages over conventional macroscopic settings. Currently, in microfluidic surface-enhanced Raman spectroscopy studies, most of the enhanced substrates composed of metallic nanostructures are directly prepared in channels. For example, Kang et al. reported a light-induced synthesis method for the preparation of silver nanoparticles embedded on the surface of ZnO nanowires integrated into microfluidic systems; Bai et al. prepared a method based on femtosecond laser-as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 杨菊平黄安寿曾祖刚黄映洲张海燕刘安平孙晓楠张欣王丹
Owner CHONGQING ENVIRONMENT & SANITATION GRP CO LTD
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