Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tumor cell apoptotic body and its preparation method and application

A tumor cell apoptosis and tumor cell technology, applied in the field of tumor cell apoptotic bodies and their preparation, can solve the problems of damage to normal cells, adverse reactions, poor targeting, etc., and achieve reduced treatment costs, good treatment effects, and production low cost effect

Active Publication Date: 2021-03-19
SUN YAT SEN UNIV
View PDF12 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cancer treatment methods mainly include surgical treatment, radiotherapy and drug therapy. However, these traditional cancer treatment methods are poorly targeted and highly toxic. While killing tumor cells, they will also damage normal cells and cause adverse reactions. Therefore, there are certain limitations

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tumor cell apoptotic body and its preparation method and application
  • Tumor cell apoptotic body and its preparation method and application
  • Tumor cell apoptotic body and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0060]One embodiment of the present invention provides a method for preparing tumor cell apoptotic bodies, comprising the following steps: culturing tumor cells in a hypoxic environment to induce tumor cell apoptosis and generate apoptotic bodies.

[0061] The hypoxic environment refers to a state where cells cannot obtain oxygen normally, and the oxygen concentration is preferably not higher than 6%, more preferably 1%-6%. The hypoxic environment can also be formed by the following method: adding cobalt chloride, defaminated ferric and / or sodium sulfite and other compounds that induce cell hypoxia in the medium for culturing tumor cells, and the addition amount is added to can up-regulate the tumor cell hypoxia-inducible factor 1α (HIF-1α).

[0062] The apoptotic bodies of tumor cells with higher activity can be obtained by the above preparation method, and the apoptotic bodies can effectively stimulate the immune activity in tumor carriers, thereby having excellent anti-tumo...

Embodiment 1

[0076] Example 1 Preparation of Tumor Cell Apoptotic Body in Hypoxic Environment

[0077] 1. Induce tumor cell apoptosis

[0078] In an oxygen-deprived environment (O 2 The concentration is 1%-6%), continue to culture breast cancer cells (MDA-MB-231) in the cell incubator for 2 days, induce cell apoptosis, produce apoptotic body, collect the cell supernatant, record as cell Supernatant A: The incubator is sterile and non-toxic, the temperature is 35°C-37°C, and the medium is a complete medium containing 10% serum. A hypoxic environment can also be created by adding to the medium a compound that induces cellular hypoxia, such as cobalt chloride, defaminated ferric, and / or sodium sulfite, in an amount that is added to upregulate hypoxia-inducible factor 1α in tumor cells (HIF-1α).

[0079] 2. Extraction and purification of apoptotic bodies

[0080] (1) Removal of cells and cell debris: Centrifuge the cell supernatant A for 1 h under the condition of a centrifugal force of 50...

Embodiment 2

[0084] 1. Induce tumor cell apoptosis

[0085] In an oxygen-deprived environment (O 2 The concentration is 1%-6%), continue to culture breast cancer cells (MDA-MB-231) in the cell incubator for 2 days, induce cell apoptosis, produce apoptotic body, collect the cell supernatant, record as cell Supernatant A: The incubator is sterile and non-toxic, the temperature is 35°C-37°C, and the medium is a complete medium containing 10% serum.

[0086] 2. Extraction and purification of apoptotic bodies

[0087] (1) Removing cells and cell debris: Centrifuge the cell supernatant A for 30 min under the condition of a centrifugal force of 500 g, discard the precipitate, take the supernatant, and record it as supernatant B;

[0088] (2) Precipitate apoptotic bodies: Centrifuge the supernatant B for 1 h under the condition of a centrifugal force of 2500 g, discard the supernatant, and obtain the precipitate;

[0089] (3) Purification of apoptotic bodies: resuspend the precipitate obtained ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a tumor cell apoptosis body and a preparation method and an application thereof. The preparation method of the tumor cell apoptosis body comprises the following steps: culturing tumor cells in an anoxic environment so as to induce the tumor cells to generate apoptosis and generate the apoptosis body. The tumor cell apoptosis body obtained by the preparation method has highactivity and excellent anti-tumor effect, can be used as a tumor vaccine for treating cancer, and has the advantages of accurate treatment targeting, low toxic and side effects and good treatment effect.

Description

technical field [0001] The invention relates to the field of anti-tumor vaccines, in particular to a tumor cell apoptosis body and its preparation method and application. Background technique [0002] In recent years, the incidence of malignant tumors has increased year by year. Although scientists have been exploring various ways to overcome tumors, the clinical cure rate of tumors has not yet shown a significant increase. Cancer treatment methods mainly include surgery, radiotherapy and drug therapy. However, these traditional cancer treatment methods are poorly targeted and highly toxic. While killing tumor cells, they will also damage normal cells and cause adverse reactions. Therefore, it has certain limitations. With the rapid development and cross penetration of related disciplines such as oncology, immunology, and molecular biology, the research on tumor immunotherapy has advanced by leaps and bounds. The US FDA has recently approved several immunotherapy projects...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09A61K39/00A61K35/13A61P35/00
CPCA61K35/13A61K39/0011A61P35/00C12N5/0693C12N2500/02
Inventor 冯敏郭羚
Owner SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com