Method for preparing neutral polysaccharide from syringa pubescens turcz

A neutral polysaccharide and crude polysaccharide technology, applied in the field of neutral polysaccharide preparation, can solve problems such as unreported activity research, and achieve the effects of high protein removal rate, large adsorption capacity and low solvent content

Inactive Publication Date: 2019-07-23
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Modern pharmacological studies have confirmed that polysaccharides are one of the active ingredients of traditional Chinese medicine. They have the effects of anti-oxidation, anti-tumor, anti-virus, enhancing immunity, lowering blood sugar and blood lipids, etc. Research results have shown that the content of polysaccharides in Qiao Linghua is relatively high, but it is harmful to Its related activity research has not been reported. In order to better develop and utilize Qiaolinghua resources, the applicant conducted in-depth research on the preparation process of its polysaccharides, and analyzed the antioxidant activity to provide scientific and experimental basis for its further product development.

Method used

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  • Method for preparing neutral polysaccharide from syringa pubescens turcz
  • Method for preparing neutral polysaccharide from syringa pubescens turcz
  • Method for preparing neutral polysaccharide from syringa pubescens turcz

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The first step, preparation of Qiao Ling flower extract:

[0035] First weigh 100g of Qiaolinghua medicinal material, soak it in 95% ethanol until the soaking solution is colorless, then dry the soaked Qiaolinghua, then crush the dried Qiaolinghua, and pass the crushed Qiaolinghua powder through 60 mesh Sieve with a vibrating sieve, add water to 20 times the weight of the medicinal material as the extraction solvent, extract with a YMNL-3000Y ultrasonic extractor for 60 minutes at 40°C, extract twice, and combine the extracts;

[0036] The second step, concentration:

[0037] Continuing from the previous step, the filtrate was recovered under reduced pressure in Anke N1100D-SB1100 rotary evaporator, the temperature of the heating bath was 65°C, the vacuum degree was 0.5MPa, the temperature of the cooling medium was 4°C, and the filtrate was concentrated under reduced pressure to 1 / 3 of the volume of the filtrate ;

[0038] The third step, alcohol precipitation:

[00...

Embodiment 2

[0047] The first step, preparation of Qiao Ling flower extract:

[0048] First weigh 100g of Qiaolinghua medicinal material, soak it in 95% ethanol until the soaking solution is colorless, then dry the soaked Qiaolinghua, then crush the dried Qiaolinghua, and pass the crushed Qiaolinghua powder through 60 mesh Sieve with a vibrating sieve, add water to 20 times the weight of the medicinal materials as the extraction solvent, extract with a YMNL-3000Y ultrasonic extractor for 30 minutes at 90°C, extract 3 times, and combine the extracts;

[0049] The second step, concentration:

[0050] Continuing from the previous step, the filtrate obtained in the previous step is decompressed to recover the solvent in a rotary evaporator, the temperature of the heating bath is 90°C, the vacuum degree is 0.8MPa, the temperature of the cooling medium is 25°C, and concentrated under reduced pressure to 1 / 5 of the volume of the filtrate ;

[0051] The third step, alcohol precipitation:

[005...

Embodiment 3

[0060] The first step, preparation of Qiao Ling flower extract:

[0061] First weigh 100g of Qiaolinghua medicinal material, soak it in 95% ethanol until the soaking solution is colorless, then dry the soaked Qiaolinghua, then crush the dried Qiaolinghua, and pass the crushed Qiaolinghua powder through 60 mesh Sieve with a vibrating sieve, add water to 20 times the weight of the medicinal materials as the extraction solvent, and extract with a YMNL-3000Y ultrasonic extractor for 45 minutes at 60°C, and extract once;

[0062] The second step, concentration:

[0063] Continuing from the previous step, recover the solvent from the filtrate obtained in the previous step under reduced pressure in a rotary evaporator. The temperature of the heating bath is 80°C, the degree of vacuum is 0.7MPa, the temperature of the cooling medium is 20°C, and concentrated under reduced pressure to 1 / 4 of the volume of the filtrate ;

[0064] The third step, alcohol precipitation:

[0065] Contin...

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Abstract

The invention provides a method for preparing neutral polysaccharide from syringa pubescens turcz. An ultrasonic extraction method provided by the invention has the characteristics of high efficiency,simplicity and convenience; a trichloroacetic acid method is short in deproteinization time, small in solvent amount, simple and feasible and high in protection removal rate; a resin method is high in decolorizing efficiency and low in cost and resin can be repeatedly used; DEAE (diethyl-aminoethanol)-cellulose has a large adsorption capacity and good stability so that the DEAE-cellulose is especially suitable for separating the neutral polysaccharide. The method for preparing the neutral polysaccharide from the syringa pubescens turcz, provided by the invention, is scientific, reasonable andfeasible; the neutral polysaccharide prepared by the invention can be further developed into an antioxidant or a food additive and the like and is suitable for being popularized and applied in a large range.

Description

technical field [0001] The invention relates to a method for preparing neutral polysaccharides, in particular to a method for preparing neutral polysaccharides from Qiao Linghua. Background technique [0002] It is known that Qiaoling flower is a plant of the genus Clove in the family Oleaceae, and its flower is a medicinal part. Qiaoling flower is very rich in resources in the Funiu Mountain area in western Henan. Folks often pick Qiaoling flower and make it into medicinal tea for drinking, which is used to treat chronic hepatitis and liver disease. Malignant diseases such as cirrhosis and liver cancer. [0003] Modern pharmacological studies have confirmed that polysaccharides are one of the active ingredients of traditional Chinese medicine. They have the effects of anti-oxidation, anti-tumor, anti-virus, enhancing immunity, lowering blood sugar and blood lipids, etc. Research results have shown that the content of polysaccharides in Qiao Linghua is relatively high, but i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 王新胜吴艳芳刘坤周冬菊陈雅维尹卫平张家萌张云蕴宋莹莹
Owner HENAN UNIV OF SCI & TECH
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