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Method for breeding high-quality female line cucumbers with hard pulp

A female line, high-quality technology, applied in the field of breeding, can solve problems such as poor palatability for fresh food, reduction of the separation of the shell and skin in the cyst cavity, and long shelf life.

Pending Publication Date: 2019-07-26
唐山市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hard pulp can make cucumbers taste crisp and tender, and hard pulp can also reduce the separation of core and skin in the cyst cavity. The product is more resistant to transportation and picking, and the shelf life of commercial melons is long. Therefore, pulp hardness is an important feature to measure the sensory quality of cucumbers, but Rough peel will cause poor palatability of fresh food

Method used

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  • Method for breeding high-quality female line cucumbers with hard pulp
  • Method for breeding high-quality female line cucumbers with hard pulp

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1: Breeding of a new germplasm TAN1819 of a high-quality female line with hard meat

[0034]The parent LF99-1 described in the present invention is a dioecious inbred line selected and bred by the Tangshan Academy of Agricultural Sciences, which is obtained from Zunhua old mange cucumber sourced from farmhouses, and obtained by multiple single plant selection methods , preferably obtained by segregation and breeding of 5 generations of selfing. The specific process is: in the spring of 1999, Zunhua old mange cucumber seeds were sown in the nursery, 26 plants emerged, and self-pollination was carried out. The individual plants were numbered and the seeds were collected separately. Among them, the 8th plant was the best, showing disease resistance, melon color green, Hard flesh, large thorns, thick flesh; In the middle of July of the same year, 20 melon seeds were sown, and a total of 18 plants emerged. Observe the heat resistance and disease resistance at the see...

Embodiment 2

[0050] Example 2: Breeding of new germplasm TAN0304 of high-quality female line with hard meat

[0051] On this basis, other hard-fleshed autumn cucumber inbred lines, such as LF99-3, and the female line TA1208 selected by Tangshan Academy of Agricultural Sciences were used for breeding in the same way as in Example 1, and the TAN0304 obtained by breeding , also has the characteristics of hard meat and high-quality all-female.

[0052] Tested by the Vegetable Research Center of Beijing Academy of Agriculture and Forestry Sciences, the new TAN0304 germplasm of hard meat and high-quality female cucumber cultivated by the present invention has a water content of 94.9%, a Vc content of 21.2mg / 100g, a soluble sugar of 3.65%, and a soluble solid content of 4.9%. , protein 1.06%.

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Abstract

The invention relates to a method for breeding high-quality female line cucumbers with hard pulp. The method comprises the steps of carrying out hybridization by using diclinous homologous strain high-quality hard-pulp inbred line LF99-1 as a female parent and adopting homozygous stable female line TA1208 as a male parent (male flower induced) to obtain a generation F1, and then using the inbred line LF99-1 as a recurrent parent for carrying out backcrossing so as to obtain second generation seeds of BC1; planting the obtained BC1 in a nursery, detecting the botany agronomic traits from the seedling stage to the ten-leaf-one-core period, reserving 2-10 sections of plants which are all female flowers, have large nodules and are good in skin color and free from diseases, and carrying out artificial pollination; by analogy, carrying out backcross transformation for 5-6 generations to obtain generation BC5 seeds; enabling the generation BC5 to undergo a systematic breeding process of self-crossing and sister-crossing by inducing male flowers so as to create a high-quality female line new germplasm TAN1819 with hard and crispy pulp. The method utilizes the inbred line LF99-1, which hasthe characteristics of being strong in plant growth vigor, hard in pulp, small in cavity, resistant to downy mildew and blight, and the like, as the recurrent parent for carrying out the backcross transformation for a plurality of generations with the female line, and combines the excellent quality and female characters of the inbred line with the hard pulp, thus creating the high-quality female line cucumber new germplasm with the hard pulp.

Description

technical field [0001] The invention relates to the technical field of breeding methods, in particular to a method for breeding hard-fleshed, high-quality female line cucumbers. Background technique [0002] Cucumber is one of the main vegetable varieties in China. With the improvement of living standards, people have higher requirements for the quality of cucumber. Fresh cucumbers require crisp, tender and fragrant taste, green and uniform fruit color, thick, hard and crisp flesh, and resistance to transportation and sales. Pulp firmness is an important trait to measure the sensory quality of cucumber. Tangshan Autumn Cucumber is deeply loved by consumers for its unique flavor and appearance. There are two local varieties of Tangshan Autumn Cucumber, one is Tangshan Rabbit Leg Cucumber and the other is Zunhua Old Scabies Cucumber. Zunhua old scab cucumber has small hard flesh, strong flavor, heat and drought resistance, downy mildew, fusarium wilt and angular spot disease...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 李玉华李聪晓苏胜宇韩靖玲付丽军宋瑞生廖贵
Owner 唐山市农业科学研究院
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