A phase microscopy imaging device and method based on structured light illumination

A technology of structured light illumination and phase microscopy, applied in the direction of optical devices, microscopes, measuring devices, etc., can solve the problem that the phase distribution of the sample cannot be quantitatively reconstructed, meet the requirements of reducing the coherence of the light source, stabilize the optical path, and improve the signal quality. The effect of the noise ratio

Active Publication Date: 2020-08-28
西安欧益光电科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Both of the above two devices form differential interference in one direction through refraction and light splitting. Although they can improve the imaging contrast of transparent samples, they cannot quantitatively reconstruct the phase distribution of the sample.

Method used

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  • A phase microscopy imaging device and method based on structured light illumination
  • A phase microscopy imaging device and method based on structured light illumination
  • A phase microscopy imaging device and method based on structured light illumination

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Embodiment 1

[0064] See figure 1 , figure 1 It is a structural schematic diagram of a phase microscopy imaging device based on structured light illumination provided by an embodiment of the present invention. The phase microscopy imaging device of this embodiment includes an illumination module, a beam modulation module and a differential interference imaging module, wherein the illumination module includes a light source 1 and a first telescope unit 2 . The light source 1 can be a laser or an LED light source. In this embodiment, the light source 1 is a laser, further a helium-neon laser. The wavelength of the light generated by the light source 1 is in the range of visible light, and the output laser power is stable, which is used to illuminate the sample to be tested. The first telescope unit 2 is used to expand and collimate the light beam generated by the light source 1 into parallel light.

[0065] The beam modulation module includes a polarizer 3, a digital beam modulation unit ...

Embodiment 2

[0095] On the basis of the above embodiments, this embodiment proposes a phase microscopy imaging method based on structured light illumination. The method adopts the phase microscopic imaging device described in the first embodiment for imaging. See Figure 4 , Figure 4 It is a flow chart of a phase microscopy imaging method based on structured light illumination provided by an embodiment of the present invention. The method includes:

[0096] S1: Obtain differential interference fringes in two different directions;

[0097] S2: Obtaining phase gradients of the sample to be measured under the differential interference fringes in the two different directions respectively;

[0098] S3: Obtain the spectral distribution of the phase image of the sample to be measured according to the phase gradient, and perform Fourier transform on the spectral distribution to obtain the phase distribution of the sample to be measured.

[0099] Further, step S1 includes:

[0100] S11: use ...

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Abstract

The invention discloses a phase microimaging device and method based on structured light illumination. The device comprises an illumination module, a light beam modulation module and a differential interferometric imaging module, wherein the illumination module includes a light source and a first telescope unit, the light beam modulation module includes a polarizing film, a light beam digital modulation unit and a second telescope unit, and the light beam digital modulation unit is used for generating striated structured light with different angles and phases; and the differential interferometric module includes a third telescope unit and an imaging unit. Light emitted by the light source successively passes through the first telescope unit, the polarizing film, the light beam digital modulation unit, the second telescope unit and the third telescope unit, and is received by the imaging unit. According to the phase microimaging device and method based on structured light illumination,illuminating light with the striated structure is produced by the digital beam modulation unit, phase gradient distribution of a to-be-measured sample in the two orthogonal directions can be obtained,and phase distribution of the measured sample is quantitatively measured.

Description

technical field [0001] The invention belongs to the technical field of microscopic imaging, and in particular relates to a phase microscopic imaging device and method based on structured light illumination. Background technique [0002] Traditional optical microscopic imaging can only obtain the intensity information of the sample, but cannot directly image or measure the phase, and the phase distribution can often reflect the three-dimensional shape, internal structure and refractive index distribution of the object, so the phase distribution of the sample can be analyzed. Imaging techniques for visual or quantitative measurements are of great interest. [0003] In 1985, Xu Yuguang from the Shanghai Institute of Optics and Fine Mechanics of the Chinese Academy of Sciences proposed in the patent CN85105355 a phase-contrast microscopy device that uses a half-mirror to split the imaging object light into two beams and perform differential interference. In 2012, Zeng Shaoqun o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G02B21/06G02B21/18G01N21/25G01B11/24
CPCG01B11/2441G01N21/25G02B21/06G02B21/18
Inventor 郑娟娟郜鹏黄韶辉邵晓鹏
Owner 西安欧益光电科技有限公司
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