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Novel hydrogel and preparing method thereof

A hydrogel and a new technology, applied in the field of new hydrogel and its preparation, can solve the problems of cytotoxicity, complex synthesis and high difficulty, and achieve the effect of reducing specific immunity

Active Publication Date: 2019-07-30
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the current research, the peptide sequences used to synthesize peptide hydrogels are longer, more than 10 amino acids, and the synthesis is complex and difficult; many synthetic short peptide molecules are likely to cause specific immunity in the body, and even have cytotoxicity

Method used

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  • Novel hydrogel and preparing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A preparation method of a novel hydrogel, which includes a hydrogel preparation step and a hydrogel forming step, specifically as follows:

[0038] Hydrogel preparation steps:

[0039] Synthetic peptides are used to prepare an aqueous gel solution. Specifically, the sequence of the synthetic peptide is: glutamine-valine-glutamic acid-valine-lysine-valine-glutamic acid-valine-lysine, the sequence abbreviation of the synthetic peptide For: QVEVKVEVK. Synthetic peptides are prepared using general preparation techniques and peptide synthesizers.

[0040] Stir the synthetic peptide with pure water to fully dissolve the synthetic peptide. Specifically, the synthetic peptide was prepared as an aqueous solution with a concentration of 10 mg / mL. Further, the pH value of the above aqueous solution was adjusted to 4.

[0041] Hydrogel Formation Steps:

[0042] The aqueous solution obtained in the hydrogel preparation step is allowed to stand at room temperature to form a gel...

Embodiment 2

[0044] A preparation method of a novel hydrogel, which includes a hydrogel preparation step and a hydrogel formation step, which is roughly the same as the preparation method provided in Example 1, except that the parameters are different, and the difference is as follows:

[0045] Hydrogel preparation steps:

[0046] The sequence of the synthetic peptide is: glutamine-valine-glutamic acid-valine-lysine-valine-glutamic acid-valine-lysine. Stir the synthetic peptide with pure water to fully dissolve the synthetic peptide. Specifically, the synthetic peptide was prepared as an aqueous solution with a concentration of 5 mg / mL. Further, the pH value of the above aqueous solution was adjusted to 4.

[0047] Hydrogel Formation Steps:

[0048] The aqueous solution obtained in the hydrogel preparation step is allowed to stand at room temperature to form a gel.

Embodiment 3

[0050]A preparation method of a novel hydrogel, which includes a hydrogel preparation step and a hydrogel formation step, which is roughly the same as the preparation method provided in Examples 1 and 2, except that the parameters are different, and the difference is as follows:

[0051] Hydrogel preparation steps:

[0052] The sequence of the synthetic peptide is: glutamine-valine-glutamic acid-valine-lysine-valine-glutamic acid-valine-lysine. Stir the synthetic peptide with pure water to fully dissolve the synthetic peptide. Specifically, the synthetic peptide was prepared as an aqueous solution with a concentration of 20 mg / mL. Further, the pH value of the above aqueous solution was adjusted to 4.

[0053] Hydrogel Formation Steps:

[0054] The aqueous solution obtained in the hydrogel preparation step is allowed to stand at room temperature to form a gel.

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Abstract

The invention discloses a novel hydrogel and a preparing method thereof, and relates to the technical field of gels. The novel hydrogel is prepared from synthetic peptide; the sequence of the synthetic peptide is glutamine-valine-glutamic acid-valine-lysine-valine-glutamic acid-valine-lysine, is easy to synthesize, and belongs to a complete amino acid sequence, and the end group of the sequence does not contain groups such as Nap-(naphthalene), Fmoc-(Fmoc-N-Me-Val-OH), Cbz-(n-carbobenzyloxy-L-serine methyl ester) and aliphatic chains; the easily-caused specific immunity can be effectively avoided or reduced, and the cell toxicity does not exist; the synthetic peptide has high gel property, and hydrogel can be rapidly formed through pH induction.

Description

technical field [0001] The invention relates to the technical field of gels, in particular to a novel hydrogel and a preparation method thereof. Background technique [0002] Peptide hydrogels have significant potential in life science fields such as tissue engineering, drug delivery, and biosensing. As a new type of material, synthetic peptides are easy to program. Some current research works control the self-assembly process of peptides by regulating the amino acid sequence of synthetic peptides to form fibers with a defined thickness to form hydrogels with tunable mechanical properties. Synthetic peptides can be designed to respond to external stimuli, such as salt ions, light, temperature, or enzymes, and the formed hydrogels can be used for blood coagulation, release of drug molecules, etc. In addition, by controlling the degradation characteristics of specific sequences of peptides in the human body, synthetic peptide-based hydrogels are ideal candidates for use as t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/02
CPCC07K7/06
Inventor 胡昊王忠琨胡坦刘凤霞吕文慧彭帮柱徐晓云潘思轶
Owner HUAZHONG AGRI UNIV
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