Unlock instant, AI-driven research and patent intelligence for your innovation.

Kelp gamma type carbonic anhydrase gene Sjgamma-CA and encoding protein and application thereof

A technology of carbonic anhydrase and protein coding, which is applied in the field of genetic engineering to achieve the effect of increasing gene expression

Active Publication Date: 2019-07-30
SHANGHAI OCEAN UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Accounting for about 90% of the total inorganic carbon content of seawater, it is the main form of seawater inorganic carbon; while CO 2 The proportion is less than 1% of the total inorganic carbon, but the photosynthetic efficiency of large seaweeds such as kelp is much higher than that of land plants (Gao & McKinley, 1994)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Kelp gamma type carbonic anhydrase gene Sjgamma-CA and encoding protein and application thereof
  • Kelp gamma type carbonic anhydrase gene Sjgamma-CA and encoding protein and application thereof
  • Kelp gamma type carbonic anhydrase gene Sjgamma-CA and encoding protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0030] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0031] 1. Materials

[0032]The kelp gametophyte material is self-made, and the temperature is 17±1℃ and the light intensity is 40μmol photons / (m 2 s) and the light / dark ratio of 16h / 8h. The medium used was PES (Starr & Zeikus, 1993), and the medium was changed every two weeks.

[0033] E. coli cloned strain DH5α, expression strain BL21(DE3) and enhanced HRP-DAB substrate chromogenic kit were purchased from Tiangen Biochemical Technology Co., Ltd.

[0034] The expression vector pET28a was purchased from Dingguo Biological Company.

[0035] Taq DNA polymerase, restriction endonucleases BamHI and NotI, T4 DNA ligase, TA cloning vector pMD19-T were purchased from TaKaRa Company.

[0036] Bio-Scale TM Mini Profinity TM IMAC Cartridges protein affinity chromatography prepacked columns were purchased from Bio-Rad Company.

[0037] The TA cloning vect...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a kelp gamma type carbonic anhydrase gene Sjgamma-CA and an encoding protein and application thereof. The cDNA sequence of the gene Sjgamma-CA is shown in SEQ ID NO:1, the DNAsequence of the gene gene Sjgamma-CA is shown in SEQ ID NO:2, the amino acid sequence of the encoding protein of the gene gene Sjgamma-CA is shown in SEQ ID NO:3, by constructing the gene Sjgamma-CA and an expression vector of the gene gene Sjgamma-CA, the amino acid sequence of the obtained recombinant protein gene rSjgamma-CA is shown in SEQ ID NO:4, and the recombinant protein rSjgamma-CA has the enzyme activity of a CO2 hydration reaction; the gene Sjgamma-CA, the encoding gene thereof and the recombinant protein rSjgamma-CA can be used for high-yield transgenic kelp crop cultivation.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the kelp gamma-type carbonic anhydrase gene Sjγ-CA, its encoded protein and its application. Background technique [0002] Kelp (Saccharina japonica) on CO 2 The absorption of water helps to alleviate greenhouse gas and water acidification. Accounting for about 90% of the total inorganic carbon content of seawater, it is the main form of seawater inorganic carbon; while CO 2 The proportion is less than 1% of the total inorganic carbon, but the photosynthetic efficiency of large seaweeds such as kelp is much higher than that of land plants (Gao & McKinley, 1994). This is because most seaweeds have an inorganic carbon concentrating mechanism (CCM) that increases CO around ribulose 1,5-bisphosphate carboxylase / oxygenase (RuBisCO) 2 concentration, so that algae can fix more CO 2 to increase its photosynthetic efficiency. In CCM, carbonic anhydrase (CA) i...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/60C12N9/88
CPCC12N9/88C12Y402/01001Y02P60/20
Inventor 周志刚许玲毕燕会
Owner SHANGHAI OCEAN UNIV