A quantum dot space tracer temperature measurement system and method
A quantum dot and tracer technology, which is applied in thermometers, heat measurement, measuring devices, etc., can solve the problems of real-time three-dimensional space positioning and tracking of quantum dots in cells.
Active Publication Date: 2020-10-27
XI AN JIAOTONG UNIV
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In terms of particle tracking, at present, most of them are tracers in a two-dimensional plane. The common fluorescent confocal scanning technology and stochastic optical reconstruction technology require layered scanning of samples, which cannot realize real-time three-dimensional spatial positioning of quantum dots in cells. track
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[0035] In describing the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", " The orientation or positional relationship indicated by "vertical", "horizontal", "top", "bottom", "inner", "outer", "side", "end", "side" etc. is based on the Orientation or positional relationship is only for the convenience of describing the present invention and simplifying the description, and does not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation of the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be interpreted as indicating or implying relative importance or implicitly specifying the quantity of indicated technical features. Thus, a feature defined as "first" and "second" may explicit...
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The invention discloses a quantum dot space tracing temperature measurement system and method. In a dark field environment, a quantum dot probe marked in a cell sample is excited by using a laser, after microscopic amplification by an objective lens, a light path is divided into two paths by a semi-reflecting and semi-transmitting mirror, one path obtains three-dimensional space position information of quantum dots dispersed in a sample cell by adopting an optical phase modulation method, and an EMCCD collects image data at different moments at a certain frequency in an image mode, so that three-dimensional space positioning and tracing of the quantum dots are realized; the second path obtains fluorescence intensity and wavelength information of the quantum dots by adopting a spectrograph,the EMCCD synchronously collects spectrum data at different moments in a spectrum mode, real-time temperatures of the quantum dots are obtained according to the photo-thermal characteristics of the quantum dots, the two EMCCDs respectively send acquired data to a computer, and the computer matches the positions of the quantum dots with the temperatures at different moments, to complete space tracing and temperature measurement of the quantum dots. High-precision three-dimensional space tracing and synchronous temperature measurement of intracellular quantum dots are realized.
Description
technical field [0001] The invention belongs to the technical field of micro-nano temperature measurement and three-dimensional tracer, and in particular relates to a quantum dot space tracer temperature measurement system and method. Background technique [0002] Cells are the basic unit of life activities, and the basis for cells to participate in life activities is the interaction between a large number of biological macromolecules inside and outside the cell. At the same time, there are various biochemical reactions in cells, and there must be temperature changes in their three-dimensional spatial structure. This temperature gradient can reflect the thermodynamic characteristics and functions of various organs of cells and even the state changes of the entire life phenomenon. If there is a way to track particles and sense real-time temperature, it will provide an effective means for studying various life processes in cells. However, most of the existing single-particle ...
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IPC IPC(8): G01K11/00G01N21/64
CPCG01K11/00G01N21/6428G01N2021/6439
Inventor 李本强朱绍冲江新兵杨欢于伟
Owner XI AN JIAOTONG UNIV