Microbial corrosion inhibitor and application method thereof
A technology for microbial corrosion and hydrate inhibitor, which is applied in the fields of botanical equipment and methods, chemicals for biological control, biocides, etc., can solve problems such as environmental protection and biological health damage, and achieve the effect of low toxicity
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Embodiment 1
[0039] The microorganism described in this example is Pseudomonas aeruginosa: MCCC1A00099 (Xiamen, China);
[0040] The medium used in this example is 2216E medium, and its composition is: NaH 2 PO 4 5.0g / L,Na 2 SO 4 1.8g / L, CaCl 2 0.55g / L, KCl 0.16g / L, Na 2 CO 3 0.08g / L, KBr 0.034g / L, SrCl 2 0.08g / L, SrBr 2 0.022g / L,H 3 BO 3 0.004g / L,NaSiO 3 0.0024g / L,NaF 0.0016g / L,NH 4 NO 3 0.008g / L, peptone 1.0g / L, ferric citrate 0.1g / L, yeast extract 0.1g / L.
[0041] 1. Antibacterial experiment:
[0042] Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) are important indicators to measure antibacterial performance. The invention adopts the micro dilution method to measure the MIC and MBC values of the catechin hydrate. The results showed that: catechin hydrate was non-toxic to MC3T3-E1 cells; catechin hydrate was toxic to 10 4 ~10 7 CFU mL -1 The minimum inhibitory concentration of Pseudomonas aeruginosa bacteria liquid is 50-20...
Embodiment 2
[0054] Desulfurizing Desulfovibrio in this example (China General Microorganism Culture Collection Center, CGMCC).
[0055] The medium composition that adopts in the present embodiment is: MgSO 4 5g / L,Na 3 C 6 h 5 o 7 ,12.5g / L,CaSO 4 2.5g / L,NH 4 Cl 2.5g / L, K 2 HPO 4 2.5g / L,NaC 3 h 5 o 3 8.75g / L, yeast extract 2.5g / L, 20mL 5% (wt%) (NH 4 ) 2 Fe(SO 4 ) 2 .
[0056] 1. Antibacterial experiment:
[0057] Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) are important indicators to measure antibacterial performance. The microdilution method was used to determine the MIC and MBC values of catechin hydrate on Desulfovibrio desulfurum. The results showed that: catechin hydrate for 10 4 ~10 7 CFU mL -1 The minimum inhibitory concentration of desulfurization Desulfovibrio bacterial liquid is 100-300ppm, and the minimum bactericidal concentration is 500-1000ppm, wherein:
Embodiment 3
[0067] Bacillus licheniformis in this example (China General Microorganism Culture Collection Center, CGMCC).
[0068] The base medium composition that adopts in the present embodiment: sucrose 10g / L, K 2 HPO 4 13.9g / L, K 2 HPO 4 2.7g / L, NaCl 1g / L, yeast extract 1g / L, NaNO 3 1g / L, MgSO 4 0.25g / L. Trace element solution 10mL; Trace element solution composition: MnCl 2 4H 2 O 18mg / L, CoCl 2 ·6H 2 O 27mg / L, H 3 BO 3 5.0mg / L, CuCl 2 2H 2 O 2.4mg / L, NaMoO 4 2H 2 O 2.3mg / L, ZnCl 2 1.9mg / L.
[0069] 1. Antibacterial experiment:
[0070] Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) are important indicators to measure antibacterial performance. The MIC and MBC values of catechin hydrate on Bacillus licheniformis were determined by microdilution method. The results showed that: catechin hydrate for 10 4 ~10 7 CFU mL -1 The minimum inhibitory concentration of bacillus licheniformis bacteria liquid is 50-200ppm, and the m...
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