Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of Caveolin-1 protein positive exosome as marker in diagnosis of non-small cell lung cancer

An exosome and protein technology, applied in the field of biomedicine, can solve the problems of poor specificity and sensitivity of lung cancer diagnosis, cumbersome operation, etc., and achieve the effect of good diagnosis efficiency.

Active Publication Date: 2019-08-06
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
View PDF9 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide the application of Caveolin-1 protein-positive exosomes as markers in the preparation of lung cancer diagnostic reagents to solve the specificity and sensitivity of lung cancer diagnosis in the prior art Poor, cumbersome operation and other problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of Caveolin-1 protein positive exosome as marker in diagnosis of non-small cell lung cancer
  • Application of Caveolin-1 protein positive exosome as marker in diagnosis of non-small cell lung cancer
  • Application of Caveolin-1 protein positive exosome as marker in diagnosis of non-small cell lung cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Serum Caveolin-1 protein positive exosome percentage analysis experiment

[0029] 1. Instruments and reagent consumables

[0030] Instruments: high-speed centrifuge, Beckman MAX-XP desktop ultracentrifuge, TLA120.2 centrifuge rotor, centrifuge supporting centrifuge tubes, NanoFCM nano flow analyzer.

[0031] Reagent consumables: 1.5ml centrifuge tube, PBS, Anti-Caveolin-1 antibody [EPR15554] (Phycoerythrin) (purchased from Abcam Company), nano flow analyzer quality control product (200nm standard blue FL PS), ultrapure water.

[0032] Exosome identification instruments and consumables: Nanosight NS300 nanometer particle size analyzer, electron microscope H-7650HITACHI microscope, vertical electrophoresis apparatus and membrane transfer device, ChemiDocCRS imager, PBS, 2% phosphotungstic acid, Anti-Caveolin-1 (610406, BD), Goat-Anti-Mouse IgG / Gold (bs-0296G-Gold 10nm, Bioss), 30% polyacrylamide, PVDF membrane, methanol, SDS, TEMED, skimmed milk powder.

[0033] 2. Coll...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides the application of Caveolin-1 protein positive exosome as a marker in the diagnosis of non-small cell lung cancer. According to the invention, the Caveolin-1 protein positive exosome can serve as a biomarker for early diagnosis and differential diagnosis of the non-small cell lung cancer and has the good diagnostic efficacy.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of exosome Caveolin-1 protein as a diagnostic marker for non-small cell lung cancer. Background technique [0002] Lung cancer has become the malignant tumor with the highest morbidity and mortality in the world, and it is on the rise. From the perspective of clinical treatment, combined with biological characteristics, lung cancer can be divided into two categories: small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). Most non-small cell lung cancers lack neuroendocrine features, histologically include adenocarcinoma (AD, 40%), squamous cell carcinoma (SQC, 30%), large cell carcinoma (10%) and other types, sensitive to radiotherapy and chemotherapy significantly lower than that of small cell lung cancer. The detection rate of NSCLC is low. Generally, stage I~IIIa can be cured clinically through surgery or adjuvant radiotherapy and chemotherapy. The "...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/574
CPCG01N33/57423G01N2333/705
Inventor 郑磊张涵安泰学司徒博李博
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
Features
  • Generate Ideas
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com