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Application and product of combined metabolic marker

A metabolic marker, combined technology, applied in biological testing, material testing products, biological material analysis, etc., can solve the problems of false positive rate, low sensitivity of liver cancer, false negative rate, etc., and achieve easy operation and optimal diagnosis. Efficacy, the effect of simplifying the detection method

Active Publication Date: 2021-01-26
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Observing whether the AFP index is elevated is often used in the auxiliary diagnosis of liver cancer, but there are certain limitations, such as: active hepatitis, pregnancy, germ cell tumors, etc. need to be ruled out first, and studies have shown that the positive rate of AFP in patients with liver cancer is between 70- Between 90%, the sensitivity of AFP to diagnose liver cancer is not high, and there is a certain false positive rate and false negative rate

Method used

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  • Application and product of combined metabolic marker
  • Application and product of combined metabolic marker
  • Application and product of combined metabolic marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Volunteers were divided into three groups: liver cancer patients, liver cirrhosis patients and healthy volunteers. The peripheral blood samples of the three groups of volunteers were collected from December 2016 to December 2018 in the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province. Peripheral blood from 27 patients with liver cancer, average age 52.78±9.42, male to female ratio 20:7; peripheral blood from 23 patients with liver cirrhosis, average age 47.83±15.10, male to female ratio 20:3; peripheral blood from 30 healthy volunteers. All volunteers obtained informed consent. The results of liver cancer in the liver cancer group were confirmed by histopathological results, and the results of liver cirrhosis in the liver cirrhosis group were confirmed by magnetic resonance imaging or B-ultrasound. The study protocol was conducted in accordance with the ethical guidelines of the 1975 Declaration of Helsinki.

[0026] After collecting th...

Embodiment 2

[0037] Liver function indicators aspartate (AST) and alanine aminotransferase (ALT) were measured by automatic biochemical analyzer, AFP was measured by electrochemiluminescence immunoassay with MICROLAB FAME microplate reader, and Spearman correlation analysis was used to compare serum 2-hydroxy-butyric acid, The correlation between the expression level of 2E-eicosenoic acid and AFP, classic liver function indexes (AST, ALT). The result is as Figure 2-3 shown. Depend on figure 2 It can be seen that we found that there is a positive correlation between the expression of 2-hydroxy-butyric acid and 2E-eicosenoic acid and AFP, and the correlation coefficients R are 0.597 (p=0.001) and 0.43 (p=0.028), respectively. Depend on image 3It can be seen that there is a positive correlation between 2-hydroxy-butyric acid and liver function index alanine aminotransferase ALT (R=0.412, p=0.036), and there is a positive correlation between 2E-eicosenoic acid and liver function index as...

Embodiment 3

[0039] Receiver operating characteristic (ROC) curve analysis was constructed to obtain the area under the curve (AUC) value of the ROC curve, and the optimal cut-off value for the diagnosis of liver cancer was determined according to the point corresponding to the maximum Youden index, and then obtained The sensitivity and specificity of diagnosing liver cancer, comparing the diagnostic performance of 2-hydroxy-butyric acid combined with 2E-eicosenoic acid and AFP in serum for liver cancer. The result is as Figure 4-6 shown.

[0040] Depend on Figure 4 and Figure 5 It can be seen that the area under the ROC curve (0.987) of 2-hydroxy-butyric acid combined with 2E-eicosenoic acid to distinguish liver cancer and cirrhosis is higher than that of AFP (0.904), so 2-hydroxy-butyric acid combined with 2E -Eicosenoic acid is more effective than AFP in distinguishing liver cancer and liver cirrhosis, and is determined by Figure 5 It can be seen that the combined diagnostic per...

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Abstract

The invention provides application and a product of a combined metabolic marker, and belongs to the technical field of laboratory medicine. The combined metabolic marker provided by the invention comprises 2-hydroxybutyric acid and 2E-eicosapentaenoic acid, can be applied to preparation of a product for detecting, diagnosing or treating liver cancer, and can be used for judging the disease condition of liver cancer in a detected object according to the expression levels of 2-hydroxybutyric acid and 2E-eicosapentaenoic acid in a serum sample of the detected object. The marker is used for distinguishing liver cancer, healthy people and liver cirrhosis patients. When the 2-hydroxybutyric acid is combined with the 2E-eicosapentaenoic acid to diagnose the liver cancer, the area under a curve ishigher than that of AFP, and the diagnosis efficiency is better.

Description

technical field [0001] The invention belongs to the technical field of laboratory medicine, and in particular relates to the application of a combined metabolic marker and its product. Background technique [0002] Liver cancer is a common malignant tumor of the digestive tract. Its incidence rate ranks 6th globally and its mortality rate ranks 3rd. In my country, its incidence rate ranks 4th and its mortality rate ranks 2nd. The number of liver cancer cases in my country is half of the total incidence and death of liver cancer in the world, and the situation is serious. Liver cancer not only has the characteristics of high morbidity and mortality, but also has difficulties in early diagnosis, early metastasis, insensitivity to radiotherapy and chemotherapy, and easy recurrence after surgery. [0003] The traditional diagnostic method is to detect Alpha-fetoprotein (AFP) in the serum. The detection method of AFP is generally to collect venous blood and separate the plasma, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/50G01N2800/52G01N2800/7028
Inventor 黄俊琪唐秀鑫邓小红何晓顺梅美华刘平娟
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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