High speed nucleic acid amplification method, and equipment and application thereof
A nucleic acid and extremely fast technology, applied in biochemical equipment and methods, specific-purpose bioreactors/fermenters, microbial measurement/inspection, etc., can solve the problem of low efficiency of PCR instruments, affecting work efficiency, and long reaction time and other issues, to achieve maximum flexibility and applicability, save time, and achieve the effect of rapid nucleic acid detection
Active Publication Date: 2019-08-09
GUANGZHOU ANGEL BIOSAFETY TECH CO LTD
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Abstract
The invention discloses a high speed nucleic acid amplification detection method. A nucleic acid amplification reaction tube is repeatedly placed in an ultrahigh temperature zone and an ultralow temperature zone for a short period; the temperature of the ultrahigh temperature zone is in a range of 100 to 150 DEG C; the temperature of the ultralow temperature zone is in a range of 10 to 40 DEG C; after 30 to 45 cycles, and the nucleic acid amplification detection is completed within 8 to 15 minutes. The invention also discloses a reactor for the high speed nucleic acid amplification, which comprises an ultrahigh temperature reaction zone with a fixed temperature in a range of 100 to 150 DEG C and an ultralow temperature reaction zone with a fixed temperature in a range of 10 to 40 DEG C. The invention also discloses an operation software system and equipment for the high speed nucleic acid amplification.
Application Domain
Bioreactor/fermenter combinationsBiological substance pretreatments +5
Technology Topic
BiologyReaction tube +4
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