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Preparation method of nano composite with molecular targeting/gene/photo-thermal treatment function

A technology of nanocomposites and molecular targeted drugs, which is applied in gene therapy, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., and can solve problems such as complexity, failure to meet clinical needs, and tumor recurrence , to achieve the effect of inhibiting proliferation, realizing efficient treatment and improving treatment effect

Inactive Publication Date: 2019-08-13
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The occurrence of cancer is the result of the joint action of many factors. The complexity of its biology makes monotherapy often fail to meet clinical needs, and the one-dimensional mechanism of action of single drug often activates or strengthens alternative pathways, promotes chemotherapy resistance, and even leading to tumor recurrence

Method used

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  • Preparation method of nano composite with molecular targeting/gene/photo-thermal treatment function
  • Preparation method of nano composite with molecular targeting/gene/photo-thermal treatment function
  • Preparation method of nano composite with molecular targeting/gene/photo-thermal treatment function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 20 mg of N-4-azido-phthalimido-chitosan CS-N 3 Add to 2 mL dimethyl sulfoxide, stir until completely dissolved, then add 4 mg Er, 16 mg Q-amine and 4 mg Cy7 to the flask. The above system was placed in a nitrogen atmosphere, and 200 μL of an aqueous solution of copper sulfate pentahydrate (20 mg / mL) and 200 μL of an aqueous solution of sodium vitamin C (15 mg / mL) were sequentially added dropwise to the flask with a 1 mL syringe. Wrap the flask with foil and stir at room temperature for three days. The reaction solution was taken out and put into a dialysis bag (8000~14000 Da) for dialysis for three days. The dialysate was taken out and freeze-dried to obtain CE7Q. with ddH 2 O Make CE7Q and CQ into 460 μg / mL and 200 μg / mL solutions respectively, then pipette 50 μL each into a centrifuge tube, mix well to obtain CE7Q / CQ solution, and pipette in a vortex state 100 μL of SV (80 μg / mL) solution was slowly added dropwise to the CE7Q / CQ solution, and incubated at room tem...

Embodiment 2

[0049] with ddH 2 O Make CE7Q and CQ in Example 1 into 100 μg / mL and 200 μg / mL solutions respectively, then draw 50 μL each into a centrifuge tube, mix well to obtain CE7Q / CQ solution, and vortex Use a pipette gun to draw 100 μL of SV (10 μg / mL) solution and slowly drop it into the CE7Q / CQ solution, and incubate at room temperature for half an hour to obtain the nanocomposite CE7Q / CQ / S.

Embodiment 3

[0051] with ddH 2 O Make CE7Q and CQ in Example 1 into 1000 μg / mL and 200 μg / mL solutions respectively, then draw 50 μL each into a centrifuge tube, mix well to obtain CE7Q / CQ solution, and vortex Use a pipette gun to draw 100 μLSV (300 μg / mL) solution and slowly add it dropwise to the CE7Q / CQ solution, and incubate at room temperature for half an hour to obtain the nanocomposite CE7Q / CQ / S.

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Abstract

The invention belongs to the field of biological medicine and relates to a preparation method of a nano composite with a molecular targeting / gene / photo-thermal treatment function. The nano composite is CE7Q / CQ / S obtained in the mode that chitosan derivatives CE7Q and CQ are mixed and then carry expression plasmids SV of Survivin shRNA. According to the method, through erlotinib connected to the chitosan derivative CE7Q, the EGFR mutant lung cancer is specifically identified; through a fluorescent molecule Cy7 connected to the chitosan derivative CE7Q, near infrared fluorescence imaging and photo-thermal treatment are conducted; the chitosan derivatives CQ and CE7Q modified through quaternary ammonium salt are mixed, then carry a gene drug SV and lower Survivin expression, in cooperation with molecular targeting / gene / photo-thermal treatment, the EGFR mutant lung cancer is efficiently treated, and the drug resistance of EGFR-TKIs is reversed.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a preparation method of a nanocomposite with molecular targeting / gene / photothermal therapy. Background technique [0002] The occurrence of cancer is the result of the joint action of many factors. The complexity of its biology makes monotherapy often fail to meet clinical needs, and the one-dimensional mechanism of action of single drug often activates or strengthens alternative pathways, promotes chemotherapy resistance, and even leading to tumor recurrence. Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) have a superior therapeutic effect initially in the treatment of EGFR-mutant non-small cell lung cancer (NSCLC), and then after a period of treatment with EGFR-TKIs, many patients Drug resistance will appear (Huang L , Fu L . Mechanisms of resistance to EGFR tyrosine kinase inhibitors[J]. Acta Pharmaceutica Sinica B, 2015, 5(5):S2211383515001045.)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K31/517A61K48/00A61K47/61A61K49/00A61P11/00A61P35/00
CPCA61K31/517A61K41/0052A61K47/61A61K48/0008A61K48/005A61K49/0034A61K49/0054A61P11/00A61P35/00A61K2300/00
Inventor 高瑜李子颖朱立胜李旭东张露徐靓陈海军
Owner FUZHOU UNIV
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