Fluorescent nanospheres with pH-responsiveness and aggregation-induced fluorescence enhancement properties and their applications

A technology of aggregation-induced fluorescence and fluorescent nanometers, applied in fluorescent nanospheres and their applications in targeted tumor imaging and disease detection, can solve problems such as reducing photobleaching efficiency, and achieve good monodispersity and good fluorescence stability. Sexual, low biotoxicity effects

Active Publication Date: 2018-08-10
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

AIE-based fluorescent probes can enhance their fluorescent signal and reduce their photobleaching efficiency by increasing the content of fluorescent molecules

Method used

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  • Fluorescent nanospheres with pH-responsiveness and aggregation-induced fluorescence enhancement properties and their applications
  • Fluorescent nanospheres with pH-responsiveness and aggregation-induced fluorescence enhancement properties and their applications
  • Fluorescent nanospheres with pH-responsiveness and aggregation-induced fluorescence enhancement properties and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Measure 5 mL of styrene (analytical grade, the polymerization inhibitor was removed by distillation under reduced pressure) and disperse it in 100 mL of deionized water, add 0.167 g of N,N,N-trimethylvinylbenzyl ammonium chloride (VBTAC) and 0.113 mL of acrylic acid (AAc). Under the protection of nitrogen at room temperature, mechanically stir (400rpm) for 30min to remove the oxygen in the reaction system. Then the temperature was gradually raised to 70° C., and 10 mL of azobisisobutylamidine hydrochloride aqueous solution with a concentration of 0.037 mmol / mL was added to initiate polymerization. The reaction was completed under nitrogen protection for 8 hours to obtain polymer nanospheres. Precipitation was obtained by high-speed centrifugation (18900 rpm), and the precipitated nanospheres were redispersed in 50 mL of deionized water to obtain a pH-responsive polymer nanosphere emulsion.

[0034] (2) Measure 5 mL of the emulsion obtained in step (1), add 5 mL of ...

Embodiment 2

[0038] (1) Measure 5 mL of styrene (analytical grade, the polymerization inhibitor was removed by distillation under reduced pressure) and disperse it in 100 mL of deionized water, add 0.125 g of N,N,N-trimethylvinylbenzyl ammonium chloride (VBTAC) and 0.127 mL of acrylic acid (AAc). Under the protection of nitrogen at room temperature, mechanically stir (400rpm) for 30min to remove the oxygen in the reaction system. Then the temperature was gradually raised to 70° C., and 10 mL of azobisisobutylamidine hydrochloride aqueous solution with a concentration of 0.037 mmol / mL was added to initiate polymerization. The reaction was carried out under the protection of nitrogen for 8 hours to obtain polymer nanospheres. Precipitation was obtained by high-speed centrifugation (18000 rpm), and the precipitated nanospheres were redispersed in 50 mL of deionized water to obtain a pH-responsive polymer nanosphere emulsion.

[0039] We found that the properties of the pH-responsive nanosph...

Embodiment 3

[0041] (1) Measure 5 mL of styrene (analytical grade, the polymerization inhibitor was removed by distillation under reduced pressure) and disperse it in 100 mL of deionized water, add 0.25 g of N,N,N-trimethylvinylbenzyl ammonium chloride (VBTAC) and 0.085 mL of acrylic acid (AAc). Under the protection of nitrogen at room temperature, mechanically stir (400rpm) for 30min to remove the oxygen in the reaction system. Then the temperature was gradually raised to 70° C., and 10 mL of azobisisobutylamidine hydrochloride aqueous solution with a concentration of 0.037 mmol / mL was added to initiate polymerization. The reaction was completed under nitrogen protection for 8 hours, and polymer nanospheres were prepared. Precipitation was obtained by high-speed centrifugation (18900 rpm), and the precipitated nanospheres were redispersed in 50 mL of deionized water to obtain a pH-responsive polymer nanosphere emulsion.

[0042] We found that the properties of the pH-responsive nanosphe...

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Abstract

The invention provides a fluorescent nano-microsphere with pH responsiveness and AIE (aggregation induced enhanced emission) property and an application of the fluorescent nano-microsphere in targeted tumor cell imaging, and belongs to the technical field of high polymer materials. Firstly, a nano-microsphere with the pH responsiveness is synthesized with a soap-free emulsion polymerization method, and the surface of the microsphere is polymerized by two types of monomers with electropositivity and electronegativity respectively, wherein the polymeric monomer with the electropositivity is a strong electrolyte; after the polymeric monomer with the electropositivity is combined with an AIE type fluorescent molecule with the electronegativity, the AIE effect of the fluorescent molecule is realized; the polymeric monomer with the electronegativity is a weak electrolyte and is subjected to protonation or deprotonation reaction under different pH conditions, and the nano-microsphere is endowed with the pH responsiveness. The surface of the nano-material is modified with an FA (folic acid) molecule through a chemical reaction, and the tumor cell targeting function is realized. Therefore, a synthesized FA-pH responsive fluorescence probe has a very broad application prospect in the fields of in-vivo targeted tumor imaging, disease detection and the like.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and in particular relates to a fluorescent nanosphere with pH responsiveness and aggregation-induced fluorescence enhancement properties and its application in targeted tumor imaging and disease detection. Background technique [0002] Cancer has always been a threat to human life and health. At present, the most effective treatment is surgical resection. However, at this stage, doctors can only rely on the difference in shape and color between tumor tissue and normal tissue to judge the lesion area, so it is difficult to completely remove cancerous tissue. By increasing the color contrast between tumor tissue and normal tissue, fluorescence imaging technology can help doctors quickly and accurately remove cancerous tissue and reduce damage to normal tissue. Therefore, fluorescent probes targeting tumor tissue imaging have attracted widespread attention. [0003] At present, nanomateria...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F212/08C08F212/14C08F220/06C08F8/32C08F8/00C09K11/06A61K49/00G01N21/64
CPCA61K49/0054A61K49/0063C08F8/00C08F8/32C08F212/08C08F2810/50C09K11/06C09K2211/1007C09K2211/1011G01N21/6402C08F226/02C08F220/06
Inventor 林权张川陈洁杨旭东孙源卿杨雪赵月杨柏
Owner JILIN UNIV
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