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Identification and detection methods for RNA specificity by boric acid-nanometer particle system

A nanoparticle and detection method technology, applied in the field of nucleic acid detection, can solve the problems of inability to distinguish between RNA and DNA, cumbersome process, etc.

Inactive Publication Date: 2019-08-13
DALIAN UNIV OF TECH
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  • Claims
  • Application Information

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Problems solved by technology

However, this method is cumbersome and cannot distinguish between RNA and DNA with the same base sequence

Method used

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  • Identification and detection methods for RNA specificity by boric acid-nanometer particle system
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  • Identification and detection methods for RNA specificity by boric acid-nanometer particle system

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Embodiment Construction

[0021] The specific implementation manner of the present invention will be further described below by taking SPR detection as an example in conjunction with the accompanying drawings.

[0022] First, the recognition DNA is immobilized on the sensing surface, and the recognition DNA can carry out base pairing with the RNA to be detected in the characteristic sequence; then, the RNA on the sensing surface is recognized and the signal is amplified by PBA-NPs. figure 1 , 2 It is a schematic diagram of the selective distinction between RNA and DNA in the PBA-NPs system in the sensor.

[0023] Specific implementation steps:

[0024] First, phenylboronic acid molecules containing thiol groups were modified on the surface of gold nanoparticles or silver nanoparticles by thiol self-assembly to form a PBA-NPs system.

[0025] Then, the recognition DNA is immobilized on the surface of the sensor chip. The recognition DNA can perform base pairing with complementary single-stranded RNA ...

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Abstract

The invention provides identification and detection methods for RNA specificity by a boric acid-nanometer particle system, and belongs to the technical field of nucleic acid detection. Phenylboronic acid molecules containing sulfydryl are modified on the surfaces of gold and silver nanometer particles through a mercaptan self-assembly mode to form the PBA-NPs system. The PBA-NPs system can be effectively combined with RNA for signal marking and amplification. The PBA-NPs system has the greatest advantage that RNA and DNA with the same base sequence can be selectively differentiated and can becombined with multiple nucleic acid detection technologies for use to achieve specificity detection on RNA of the specific sequence.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid detection, uses boric acid molecule-modified nanoparticles (PBA-NPs) to identify and amplify RNA, and can be used for multiple detection platforms, such as surface plasmon resonance (Surface plasmon resonance, SPR) detection, fluorescence Detection, chromatographic detection, surface-enhance Raman Scattering (SERS) detection, electrochemical detection, etc. Background technique [0002] Phenylboronic acid (PBA) can reversibly combine with cis-diols. By changing the pH, the reversible combination and separation of boric acid and diols can be achieved. Sugars and their derivatives (such as glycoproteins, ribonucleic acids, etc.) play an important role in the physiological activities of the human body. Due to the polyhydric properties of sugars, some sugars and their derivatives conforming to cis-ortho diols can specifically bind to boric acid, while sugars with a single hydroxyl structure hav...

Claims

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Application Information

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IPC IPC(8): C12Q1/6825C12Q1/682
CPCC12Q1/6825C12Q1/682
Inventor 彭伟钱思宇
Owner DALIAN UNIV OF TECH
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