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A kind of method that extracts cationic anthocyanin from blue indigo fruit

A kind of technology of anthocyanin and indigo fruit, which is applied in the field of plant anthocyanin, can solve the problems of complex extraction process and long time of cationic anthocyanin, achieve the effect of protecting the biological activity of anthocyanin and enriching the extraction method

Active Publication Date: 2021-04-02
GREATER KHINGAN RANGE CHAOYUE WILD BERRY DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the technical problem that the existing cationic anthocyanin extraction process is complicated and takes a long time, the present invention provides a method for extracting cationic anthocyanin from the indigo fruit. The extraction method provided by the present invention has the advantages of short extraction time and high extraction efficiency. Advantages, the extraction method of the present invention provides a new effective way for extracting cationic anthocyanins from indigo fruit and related natural plants containing anthocyanins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The present embodiment extracts the method for cationic anthocyanin from blue indigo fruit and comprises the following steps:

[0024] Step 1: Grinding the blue indigo fruit, adding low temperature water to obtain a solid-liquid mixture, the mass ratio of the low temperature water to the blue indigo fruit is 1, and the temperature of the low temperature water is controlled at 1°C;

[0025] Step 2, the mixture is pressurized, ultrasonically treated to break the cell wall of the plant of Cyanocarpus edulis, and the filtrate solution is obtained by suction filtration, the ultrasonic treatment time is 10min, and the pressure of the autoclave container is 5Mpa;

[0026] Step 3: pressurize the filtrate solution, perform enzymatic hydrolysis reaction under ultrasonic to obtain cationic anthocyanin, separate from water with enzyme, and vacuum freeze-dry to obtain cationic anthocyanin finished product. The pressure is 3Mpa, the enzyme is glycoside hydrolase, the ultrasonic enzym...

Embodiment 2

[0028] The present embodiment extracts the method for cationic anthocyanin from blue indigo fruit and comprises the following steps:

[0029] Step 1: Grinding the edulis, adding low-temperature water to obtain a solid-liquid mixture, the mass ratio of the low-temperature water to the edulis is 1.5, and the temperature of the low-temperature water is controlled at 1°C;

[0030] Step 2, the mixture is pressurized, ultrasonically treated to break the cell wall of the plant of Cyanocarpus edulis, and the filtrate solution is obtained by suction filtration, the ultrasonic treatment time is 30min, and the pressure of the autoclave container is 5Mpa;

[0031] Step 3: pressurize the filtrate solution, perform enzymatic hydrolysis reaction under ultrasonic to obtain cationic anthocyanin, separate from water with enzyme, and vacuum freeze-dry to obtain cationic anthocyanin finished product. The pressure is 3Mpa, the enzyme is glycoside hydrolase, the ultrasonic enzymolysis time is 1.5h,...

Embodiment 3

[0034] The present embodiment extracts the method for cationic anthocyanin from blue indigo fruit and comprises the following steps:

[0035] Step 1: Grinding the blue indigo fruit, adding low temperature water to obtain a solid-liquid mixture, the mass ratio of the low temperature water to the blue indigo fruit is 2, and the temperature of the low temperature water is controlled at 1°C;

[0036] Step 2, the mixture is pressurized and ultrasonically treated to break the cell wall of the plant of Cystaceae, and the filtrate solution is obtained by suction filtration. The ultrasonic treatment time is 35min, and the pressure of the autoclave container is 5Mpa;

[0037] Step 3: pressurize the filtrate solution, perform enzymatic hydrolysis reaction under ultrasonic to obtain cationic anthocyanin, separate from water with enzyme, and vacuum freeze-dry to obtain cationic anthocyanin finished product. The pressure is 3Mpa, the enzyme is glycoside hydrolase, the ultrasonic enzymolysis...

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Abstract

The invention relates to a method for extracting a cationic anthocyanin from lonicera caerulea fruits. The method comprises the following steps: crushing the lonicera caerulea fruits, performing mixing by using low-temperature water, destroying plant cell walls under the action of a water press kettle and an ultrasonic device, performing suction filtration to obtain a filtrate aqueous solution containing the anthocyanin, performing an enzymatic hydrolysis reaction on the filtrate aqueous solution under the action of pressurization and ultrasonic waves, performing enzyme-water separation to obtain a cationic anthocyanin aqueous solution, and performing vacuum freeze drying to obtain a finished product. According to the method provided by the invention, pressure extrusion, a pushing effect and an ultrasonic cavitation effect are utilized in the process of cell wall destruction and anthocyanin enzymatic hydrolysis, so that the extraction speed and extraction efficiency of the anthocyaninare greatly improved, the finished product containing 90% or more of the cationic anthocyanin can be obtained, the extraction time can be shortened to within 3 h, and the method provides a novel and effective way for extraction of the cationic anthocyanin from related plants containing the anthocyanin.

Description

technical field [0001] The invention belongs to the technical field of plant anthocyanins, and in particular relates to a method for extracting cationic anthocyanins from L. indigo fruit. Background technique [0002] Anthocyanin is a pure natural anti-aging nutritional supplement. Studies have shown that it is the most effective antioxidant found by humans today. Its antioxidant performance is 50 times higher than vitamin E and 200 times higher than vitamin C. Anthocyanins are also known as "oral skin cosmetics", which can enhance skin immunity and deal with various allergic symptoms. They are currently the most effective antioxidant substances in nature. It can not only prevent the early generation of skin wrinkles, but also maintain normal cell connections, stabilize blood vessels, enhance microvascular circulation, improve the flow of capillaries and veins, and then achieve rapid healing of abnormal skin. At the same time, anthocyanin is also a natural sun cover, which ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 章结兵陈勃任秀彬汪广恒时慧
Owner GREATER KHINGAN RANGE CHAOYUE WILD BERRY DEV