Method for isolating and identifying effective degrading fungi for corn stalks

A technology of corn stalks and identification methods, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., to achieve the effects of accurate identification results, improved accuracy, accurate separation and identification

Pending Publication Date: 2019-08-16
HONGHE COLLEGE
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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for the separation and identification

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Embodiment Construction

[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0021] The invention provides a separation and identification method for highly efficient corn stalk degrading fungi. Samples are selected, (1) corn stalks in different regions and with different degrees of decomposition are selected, and five different sampling points are set, (2) corn stalks without signs of decomposition are selected. Straw, dry naturally, pass through a 40-mesh sieve after crushing, store the sifted powder in a ziplock bag, sterilize and dry before use; an...

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Abstract

The invention discloses a method for isolating and identifying effective degrading fungi for corn stalks. The method includes: selecting samples, performing inoculated culture, screening and culturingthe fungi, through four screening and culturing mediums including a fungus screening and culturing medium, a Congo red cellulose screening and agar culturing medium, a seed culturing medium and an effective corn stalk degrading liquid state fermentation culturing medium, culturing the fungi obtained from multiple times of activation, preliminarily screening cellulose degrading fungi, observing growth form of the cellulose degrading fungi, performing molecular biological identification, subjecting fungus strains obtained from morphological identification to PCR (polymerase chain reaction) detection, and establishing a phylogenetic tree for obtained results so that to-be-tested fungus strains can be identified more correctly. The fungus screening and culturing medium is utilized to screen the effective corn stalk degrading fungi, and the Congo red cellulose screening and agar culturing medium, the seed culturing medium and the effective corn stalk degrading liquid state fermentation culturing medium are utilized to screen the effective degrading fungi. By utilizing different screening and culturing mediums to culture, the required fungi can be obtained more accurately, and accuracyof an experiment can be improved. Through strict sterilization during purification, identification results are enabled to be more accurate.

Description

technical field [0001] The invention relates to the field of biodegradation, in particular to a method for isolating and identifying a fungus for efficiently degrading corn stalks. Background technique [0002] As a large agricultural country, China is rich in crop straw resources. The amount of crop straw resources that can be collected every year exceeds 900 million tons, of which corn straw accounts for one-third of the total. Now the rural cultivated land generally suffers from the decline of soil fertility. The phenomenon seriously restricts the development of modern agriculture. Straw returning to the field is an effective method to improve the utilization efficiency of crop straw, improve soil fertility, and improve the soil microenvironment. At present, the comprehensive utilization rate of corn straw in Mengzi is still very low and cannot be realized. Efficient utilization of corn stalks after returning to the field. Most farmers still use direct burning of corn sta...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
CPCC12Q1/045
Inventor 李杰杨萍王田涛杨若鹏苏一兰周银丽
Owner HONGHE COLLEGE
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