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Application of antibacterial peptide Lchamp2-3 in anti-tumor cell proliferation medicine

A technology for anti-tumor cells and tumor cells, applied in the application field of antibacterial peptide Lchamp2-3 in anti-tumor proliferation drugs, which can solve the problems of limited curative effect data

Active Publication Date: 2019-08-27
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antimicrobial peptides are just beginning to enter the oncology field, so efficacy data are relatively limited, however, safety data in infectious disease trials, albeit indirect, suggest that antimicrobial peptides are well tolerated by patients

Method used

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  • Application of antibacterial peptide Lchamp2-3 in anti-tumor cell proliferation medicine
  • Application of antibacterial peptide Lchamp2-3 in anti-tumor cell proliferation medicine
  • Application of antibacterial peptide Lchamp2-3 in anti-tumor cell proliferation medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The antimicrobial peptide Lchamp2-3 was found in large yellow croaker in vivo, and the corresponding amino acid sequence of its mature peptide was: GSPARCRFCCRCCPGMRGCGICCRF. For the specific method of obtaining it, please refer to the literature: Mu Yinnan, Huo Jieying, GuanYanyun, Fan Dingding, Xiao Xiaoqiang, Wei Jingguang, Li Qiuhua, Mu Pengfei, AoJingqun, Chen Xinhua. An improved genome assembly for Larimichthys croceareveals hepcidin gene expansion with diversified regulation .[J].Communications biology,2018,1.

[0024] Dissolve 2 mg of Lchamp2-3 lyophilized powder in 1 mL of PBS, and use it as Lchamp2-3 mother solution for later use.

Embodiment 2

[0026] Cytotoxicity Test:

[0027] Dissolve 2.5 g of MTT powder in 50 mL of PBS solution to prepare a 10-fold concentration of MTT mother liquor with a concentration of 50 mg / mL, and filter to sterilize. Take out the mother solution when used, and dilute ten times with the corresponding serum-free and phenol red-free medium to become the MTT working solution.

[0028] MDA-MB-231 cells were digested and blown into a uniform cell suspension, and the cell concentration in the suspension was calculated with a cell counting plate, and diluted to 80,000 cells per milliliter, and then spread into a 96-well plate to control the number of cells per well. Into 100μL cell suspension, 8000 cells. After incubating overnight at 37°C, add Lchamp2-3 mother solution, dilute to final concentrations of 0, 10, 20, 30, 40, and 50 μg / mL, and set 5 wells for each concentration to repeat. Put them back into a constant temperature incubator at 37°C for cultivation. After 24, 48, and 72 hours of drug...

Embodiment 3

[0034] Lchamp2-3 induced cell apoptosis, MDA-MB-231 cells in good growth state were taken, digested with trypsin and counted. According to 1.5×10 5 cell / well Cells were seeded into 6-well plates and cultured overnight in an incubator.

[0035] After the cells were completely adhered to the wall, the old medium was discarded and washed 3 times with PBS. Add L-15 complete medium with Lchamp2-3 content of 25 μg / mL to each well, incubate for 24, 48, and 72 hours respectively, discard the drug-containing medium, wash with PBS three times, digest and collect cells, and centrifuge at 1800 rpm for 5 min , discard the supernatant, and collect the cell pellet at the bottom of the centrifuge tube. Set up two single positive tubes, add 5 μL Annexin V-FITC and 10 μL PI respectively, add binding buffer buffer to a total volume of 500 μL in each tube; set negative control tubes, add 500 μL binding buffer buffer; experimental group and blank The control group needs to add 5 μL Annexin V-FI...

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Abstract

The invention discloses application of antibacterial peptide Lchamp 2-3 in an anti-tumor cell proliferation medicine. In vitro cell experiments show that the antibacterial peptide Lchamp2-3 derived from pseudosciaena crocea has significant inhibiting effect on cancer cell proliferation and apoptosis and cell colony formation. The antibacterial peptide Lchamp 2-3 can be applied in the anti-tumor cell proliferation medicine to develop new medicine candidates for cancer prevention and treatment.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of antibacterial peptide Lchamp2-3 in anti-tumor proliferation drugs. Background technique [0002] Anticancer drugs are currently one of the hotspots of research. Genotoxic and cytotoxic drugs developed from natural products or synthetic chemistry are designed to curb the uncontrollable growth of cancer cells. Although the strategy of killing circulating cells by such chemotherapeutic drugs has significantly improved the survival rate of cancer patients, the cells The indiscriminate nature of toxicity also creates serious adverse side effects for patients and increases the risk of secondary cancers. [0003] Antimicrobial peptides (AMPs) are small molecular weight oligopeptides, generally composed of 5-40 amino acid residues, which are a class of proteins with broad-spectrum antibacterial activity and are considered to be effective drugs against var...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61P35/00
CPCA61K38/1706A61P35/00
Inventor 余素红陈新华吴文静母尹楠刘敏江舟
Owner FUZHOU UNIV
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