Artificial propagation method of oxygymnocypris stewartii
A technology of artificial breeding and naked carp, applied in fish farming, application, climate change adaptation, etc., can solve the problems of habitat and living environment deterioration of the carp, and achieve the promotion of aquaculture development, strong operability, and protection and the effects of using wild resources
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Embodiment 1
[0028] A kind of artificial propagation method of pointed naked carp, comprises the following steps:
[0029] A. Broodstock selection: In April, catch the wild carp that has entered the IV stage in the developmental period as artificial breeding parents. After the parents are sterilized, the female and male fish are separated for temporary breeding;
[0030] B. Artificial induced labor: using luteinizing hormone-releasing hormone analog LRH-A 2 and carp pituitary PG mixture as an oxytocic agent, LRH-A 2 The dosage of PG mixture is 10ug / kg, and the dosage of PG mixture is 8mg / kg; adopt the double injection method to induce labor for the female carp;
[0031] C. Artificial insemination: use anhydrous dry method for insemination. After the female fish has an effect time of 48 to 72 hours, artificially squeeze the eggs of the female fish, squeeze the eggs of the pointed naked carp into a dry white porcelain bowl, and pour 3 ~ After 4 drops of male semen, stir thoroughly for 30 s...
Embodiment 2
[0035] This embodiment is on the basis of embodiment 1:
[0036] It also includes postpartum care of broodstock: after sperm collection and egg squeezing, broodstock are soaked in 1% glucose solution for 1 hour, and then each fish is injected with gentamicin, and the broodstock after injection is transferred to the flowing water cultivation tank , using small cold-water fish as bait fish to lure them to eat.
Embodiment 3
[0038] This embodiment is on the basis of embodiment 1:
[0039] It also includes postpartum care of broodstock: after sperm collection and egg squeezing, broodstock are soaked in 1% glucose solution for 1 hour, and then each fish is injected with gentamicin, and the broodstock after injection is transferred to the flowing water cultivation tank , using small cold-water fish as bait fish to lure them to eat.
[0040] The dose of the injected gentamicin is 0.2mg / kg body weight.
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