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Preparation method for artificial cells containing cytoplasm-like substance

A cytoplasmic and artificial technology, applied in the field of artificial cell preparation, can solve the problem that artificial cells are aqueous solutions

Active Publication Date: 2019-08-30
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention solves the problem that the interior of the existing artificial cells is almost an aqueous solution, and provides a method for preparing artificial cells containing cytoplasm

Method used

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  • Preparation method for artificial cells containing cytoplasm-like substance
  • Preparation method for artificial cells containing cytoplasm-like substance
  • Preparation method for artificial cells containing cytoplasm-like substance

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specific Embodiment approach 1

[0018] Specific embodiment 1: In this embodiment, a method for preparing artificial cells containing cytoplasm is carried out according to the following steps:

[0019] 1. Preparation of cytoplasmic sol:

[0020] Mix agarose and sucrose to obtain agarose-sucrose mixed sol, add distilled water to the agarose-sucrose mixed sol, and soak for 20-30 minutes to obtain a mixed solution, place the mixed solution on a heating plate and heat until the mixed solution boils, Then stop heating, and let it stand at room temperature to obtain a cytosol-like solution;

[0021] The mass ratio of the agarose to sucrose is 1:(4~7); the volume ratio of the mass of the agarose-sucrose mixed sol to distilled water is 60mg:(1~1.2)mL;

[0022] 2. Clean the ITO glass electrode:

[0023] Immerse the ITO glass electrode in ethanol solution, ultrasonically clean it for 10min-15min, then immerse it in distilled water, ultrasonically clean it for 10min-15min, and finally dry it with nitrogen to obtain th...

specific Embodiment approach 2

[0027] Embodiment 2: This embodiment differs from Embodiment 1 in that the mass ratio of agarose to sucrose in step 1 is 1:(4-5). Others are the same as in the first embodiment.

specific Embodiment approach 3

[0028] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that the mass ratio of agarose to sucrose in step 1 is 1:(5-7). Others are the same as in the first or second embodiment.

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Abstract

The invention relates to a preparation method for artificial cells containing a cytoplasm-like substance and solves the problem that the interior of existing artificial cells is almost aqueous solution. The preparation method comprises the following steps: firstly, preparing cytoplasm-like sol; secondly, cleaning an ITO glass electrode; and thirdly, preparing phospholipid vesicles containing the cytoplasm-like substance, and obtaining the artificial cells containing the cytoplasm-like substance. The preparation method provided by the invention is used for preparation of the artificial cells containing the cytoplasm-like substance.

Description

technical field [0001] The present invention relates to a method for preparing artificial cells. Background technique [0002] At present, the research on the preparation of artificial cell models with phospholipids as the main raw material has been reported, but the interior of artificial cells is almost an aqueous solution, while the interior of real cells is a colloid with viscosity, and the micro-reactions in cells currently studied do not have real environmental characteristics. Therefore, it is necessary to construct a liquid environment close to the inside of the real cell, that is, a colloidal environment with viscosity, so as to lay the foundation for a more realistic study of the microreactions inside the cell. Contents of the invention [0003] The invention aims to solve the problem that the interior of the existing artificial cells is mostly aqueous solution, and provides a preparation method of the artificial cells containing cytoplasm. [0004] A method for...

Claims

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Application Information

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IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 宗薇张旭男
Owner QIQIHAR UNIVERSITY
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