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Biosensor and method for detecting microcystin

A technology of biosensors and microcystins, which is applied to instruments, measuring devices, scientific instruments, etc., can solve the problems of difficulty in realizing in-situ real-time detection of microcystins, high testing costs, complicated pretreatment, etc., and achieves a simple structure. , reduce the difference between batches, the effect of good application prospects

Pending Publication Date: 2019-08-30
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for detecting microcystins in order to overcome the disadvantages of high testing cost, complicated pretreatment, time-consuming operation, and difficulty in real-time detection of microcystins in situ when detecting microcystins in the prior art. biosensors and methods

Method used

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  • Biosensor and method for detecting microcystin
  • Biosensor and method for detecting microcystin
  • Biosensor and method for detecting microcystin

Examples

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Effect test

Embodiment 1

[0037] A biosensor for the detection of microcystins, such as figure 1 , figure 2 As shown, it includes a biosensor housing 1, a power supply module 2 located inside the biosensor housing 1, an incubation module 3, an immune reaction module 4, a fluorescence excitation module 5 and a signal acquisition module 6 located at the top of the biosensor housing 1 ; The power supply module 2 is arranged at the bottom of the biosensor housing 1, and is connected to the incubation module 3 and the fluorescence excitation module 5 respectively; Connection composition; one side of the biosensor housing 1 is provided with a temperature display screen 7, and the temperature display screen 7 is connected to the intelligent temperature controller 31; Composed of chips 42; the fluorescent excitation module 5 is located on the inside of the top of the biosensor housing 1, and is composed of an LED array, an excitation filter, and an emission filter. The LED array and the top of the biosensor ...

Embodiment 2

[0041] A method for detecting microcystins, comprising the steps of:

[0042] S1. Preparation of microcystin immunosensor based on nano-magnetic beads;

[0043] S2. Using the above-mentioned biosensor to measure the fluorescence intensity produced by the biosensor under the standard series of microcystin concentrations, and draw a linear relationship curve between the microcystin concentration and the fluorescence intensity;

[0044] S3. Use the above-mentioned biosensor to detect the microcystin in the water, and calculate the concentration of the microcystin in the sample by using the linear relationship curve drawn in step S2.

Embodiment 3

[0046] Example 3 Preparation of Microcystin Immunosensor Based on Nano Magnetic Beads

[0047] 1. Activation of magnetic beads

[0048] Take 500 μL of magnetic beads and place them in a 1.5mL EP tube, place them in a magnetic separation rack, enrich the magnetic beads, and remove the supernatant; add 1 mL of 1mM hydrochloric acid solution pre-cooled at 4°C, vortex for 15 seconds, and place in a magnetic separation rack for enrichment Magnetic beads, remove supernatant.

[0049] 2. Protein coupling

[0050] Add 500 μL of protein solution (MC-LR antigen at a concentration of 250 μg / mL), vortex for 30 s, and mix well. Put the EP tube on the vortex oscillator, adjust to the lowest gear, automatic mode, and oscillate for 1-1.5h.

[0051] Wherein, the ratio of the nano magnetic beads to the microcystin antigen coated on the surface is 1:1.

[0052] 3. Separation

[0053] Use a magnetic separation rack to enrich the magnetic beads and save the flow-through.

[0054] 4. Cleaning...

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Abstract

The invention discloses a biosensor and method for detecting microcystin. The biosensor comprises a biosensor housing, a power supply module, an incubation module, an immunological reaction module anda fluorescence excitation module arranged in the biosensor housing, and a signal acquisition module arranged at the top of the biosensor housing, the power supply module is arranged at the bottom ofthe biosensor housing and separately connected with the incubation module and the fluorescence excitation module, the incubation module is arranged below the immunological reaction module, and the fluorescence excitation module is arranged at the inner side of the top of the biosensor housing. The biosensor is advantaged by simple structure, low cost, convenient carrying and simple operation, real-time onsite rapid detection of the microcystin with high precision can be performed, the biosensor is applicable to onsite detection of the MC-LR, and a good application prospect is achieved.

Description

technical field [0001] The invention relates to the technical field of microcystin detection, in particular to a biosensor and method for detecting microcystin. Background technique [0002] Microcystins (MCs) are a cyclic heptapeptide compound produced by cyanobacteria blooms, such as Anabaena and Astringentia, which have strong hepatotoxicity and can strongly inhibit cellular protein phosphatase 1 (PP1) and 2A (PP2A) activities, impairing their regulatory function in cells, thereby triggering primary liver cancer. Studies have shown that 25% to 70% of cyanobacteria blooms will release toxins, among which microcystins are highly toxic, and can enter the food chain through drinking water or accumulated in aquatic products, thereby entering the human body directly or indirectly, posing a threat to human health. Great threat. Microcystins are widely distributed in natural waters, and outbreaks are seasonal, which requires that the detection method can be continuously analyze...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/535G01N33/543
CPCG01N21/6402G01N33/535G01N33/54326
Inventor 雷红涛关甜李向梅沈兴杨金易肖治理徐振林孙远明
Owner SOUTH CHINA AGRI UNIV
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