Single-wavelength two-photon STED and dual-wavelength single-photon STED coupled imaging device and method

Abstract

The invention relates to a single-wavelength two-photon STED and dual-wavelength single-photon STED coupled imaging device comprising a femtosecond laser device, a picosecond laser device, an I electric shutter, an I half wave plate, an I optical splitter, an II optical splitter or an I reflector switching device, an II half wave plate, an I quarter wave plate, an II electric shutter, an II reflector, an III reflector, an I relay reflector group, an IV reflector, an I dichroic mirror, a V reflector, an II relay reflector group, a VI reflector, a glass rod, an III half wave plate, a polarizer,a VII reflector, an I lens, a polarization maintaining optical fiber, an II lens, a VIII reflector, a phase plate, an IX reflector, an enhancing optical path reflector group and a confocal scanning microscope. The imaging device provided by the invention can be switched to the single-wavelength two-photon STED imaging system and the dual-wavelength single-photon imaging system in a time-sharing manner according to needs.

Description

technical field [0001] The invention relates to the technical field of microscopic imaging, in particular to a single-wavelength two-photon STED and dual-wavelength single-photon STED coupling imaging device and method. Background technique [0002] Due to the diffraction limit of the resolution of traditional fluorescence microscopes, some super-resolution imaging techniques that break through the diffraction limit of resolution have been developed in the past decade, such as stimulated radiation depletion microscopy (STED) and structured illumination microscopy (structured illumination microscopy). , SIM), photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), etc. Among them, STED is based on the laser scanning confocal fluorescence microscope, which introduces another ring-shaped depletion light with a wavelength longer than the excitation light. A focused laser beam excites the fluorophore to a high energy state (excited st...

AI Technical Summary

Problems solved by technology

In 2011, Teodora Scheul and others proposed the single-wavelength two-photon STED technology, but the experiment only used the solution sample and did not involve cell experiments

There is no single-photon and two-photon STED imaging platform for researchers to choose corresponding single-photon or two-photon STED imaging according to their research needs

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