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Preparation method for tissue material enriching autologous stem cells to fill in recesses

A tissue material and stem cell technology, applied in the field of stem cells, can solve problems such as complications, achieve high success rate, increase success rate, and high activity

Inactive Publication Date: 2019-09-06
朗姿赛尔生物科技(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the prosthesis is a foreign body, various complications occur after implantation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for preparing a tissue material enriched with autologous stem cells to fill depressions, comprising: S1. extracting autologous adipose-derived mesenchymal stem cells; S2. amplifying the mesenchymal stem cells and putting them in a differentiation medium to obtain adipocytes, which are formulated into adipocytes The adipocyte fluid includes adipocytes and a small amount of undifferentiated stem cells, and the culture medium includes glucose 20mM, nanometer iron ferric oxide particles 200μg / mL, insulin 20nM, penicillin 60U / L, streptomycin 100U / mL L, the balance is L-DMEM medium; S3. Mix adipocyte fluid with type I collagen to obtain tissue material. The nano ferric oxide particles are modified iron ferric oxide. The modification method of the nano-ferric oxide is: S11. In argon at 80°C, to FeCl 2 and FeCl 3 Add NaOH solution to the mixed solution, and stir vigorously for 3.5h to obtain Fe 3 o 4 , adding 0.15g / mL polyethyleneimine to obtain the Fe 3 o 4 Nano...

Embodiment 2

[0027] A method for preparing a tissue material enriched with autologous stem cells to fill depressions, comprising: S1. extracting autologous adipose-derived mesenchymal stem cells; S2. amplifying the mesenchymal stem cells and putting them in a differentiation medium to obtain adipocytes, which are prepared into adipocytes The adipocyte fluid includes adipocytes and a small amount of undifferentiated stem cells, and the differentiation medium includes glucose 15mM, nano iron ferric oxide particles 60μg / mL, insulin 15nM, penicillin 50U / L, streptomycin 90U / L L, the balance is L-DMEM medium; S3. Mix adipocyte fluid with type I collagen to obtain tissue material. The nano ferric oxide particles are modified iron ferric oxide. The modification method of the nanometer ferroferric oxide is: S11. at 75°C in argon, to FeCl 2 and FeCl 3 Add NaOH solution to the mixed solution, and stir vigorously for 3h to obtain Fe 3 o 4 , adding 0.1g / mL polyethyleneimine to obtain the Fe 3 o 4...

Embodiment 3

[0029] A method for preparing a tissue material enriched with autologous stem cells to fill depressions, comprising: S1. extracting autologous adipose-derived mesenchymal stem cells; S2. amplifying the mesenchymal stem cells and putting them in a differentiation medium to obtain adipocytes, which are prepared into adipocytes The adipocyte fluid includes adipocytes and a small amount of undifferentiated stem cells, and the differentiation medium includes glucose 25mM, nanometer iron ferric oxide particles 500μg / mL, insulin 25nM, penicillin 70U / L, streptomycin 110U / L L, the balance is L-DMEM medium; S3. Mix adipocyte fluid with type I collagen to obtain tissue material. The nano ferric oxide particles are modified iron ferric oxide. The modification method of the nano ferric oxide is: S11. In argon at 90°C, add FeCl 2 and FeCl 3 NaOH solution was added to the mixed solution, and after vigorous stirring for 4h, Fe 3 o 4 , adding 0.2g / mL polyethyleneimine to obtain the Fe 3 o...

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PUM

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Abstract

The invention relates to the field of stem cells, and provides a preparation method for a tissue material enriching autologous stem cells to fill in recesses. The preparation method is used for solving the problems of easy rejection reaction of body recess issue filling. The preparation method includes the following steps: S1, extracting autologous adipose tissue-derived mesenchymal stem cells; S2, putting the mesenchymal stem cells into a differentiation medium after amplification to obtain adipocyte, and preparing the adipocyte into an adipocyte fluid, wherein the adipocyte fluid includes the adipocyte and a small amount of undifferentiated stem cells, the differentiation medium includes 15-25 mM of glucose, 60-500 [mu]g / mL of nano ferriferrous oxide particles, 15-25 nM of insulin, 50-70U / L of penicillin, and 90-110 U / L of streptomycin, with the balance being an L-DMEM culture medium; and S3, mixing the adipocyte fluid and type I collagen so that the issue material can be obtained.Through the differentiation of the adipose tissue-derived mesenchymal stem cells, enough amount of adipose tissues used for filling can be obtained, and the obtained adipocyte is high in activity andrelatively complete, so that high success rates of filling operations can be achieved.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a method for preparing tissue materials for enriching autologous stem cells to fill depressions. Background technique [0002] Due to reasons such as race, genetics, accidents and nutrition, some people may have certain depressions on their bodies. Filling, the filling of hollows in the body with a certain component, can have an immediate effect on hollowing out the hollows. At present, the methods for filling medical depressions at home and abroad can be roughly divided into injection methods and surgical methods. The injection method uses autologous fat particles; while the surgical method uses a silicon capsule prosthesis to be inserted into the depression. [0003] Autologous fat injection has been carried out for many years, but because of its absorption rate of more than 50% and possible complications such as fat liquefaction, infection, and hematoma, the cysts caused by injecte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077
CPCC12N5/0653C12N2500/24C12N2500/34C12N2501/33C12N2533/54
Inventor 陈忠平
Owner 朗姿赛尔生物科技(广州)有限公司