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Application of sulfated curdlan

A sulfated and polysaccharide technology, applied in the field of medicine, can solve the problems of difficult to break the immune tolerance of tumor microenvironment, difficult to TAA-specific cellular immune response, and reduce the effect of TAA-sensitized DC vaccine.

Pending Publication Date: 2019-09-10
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since most TAAs are the body's own protein components, it is difficult to stimulate the body's innate immune response by the immune stimulatory signal generated by pure TAA, and it is difficult to initiate TAA-specific cellular immune response by inducing the maturation and activation of DC, so it is difficult to break the immune system of the tumor microenvironment. Tolerance, reduced efficacy of TAA-sensitized DC vaccine

Method used

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  • Application of sulfated curdlan
  • Application of sulfated curdlan
  • Application of sulfated curdlan

Examples

Experimental program
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Effect test

Embodiment

[0033] Study on the therapeutic effect of DC vaccine with CURS as adjuvant on liver cancer model mice

[0034] 1. Establishment of liver cancer mouse model and animal grouping

[0035] (1) BALB / c mice with stable growth of ascites tumors were taken, and ascites tumors were collected in an ultra-clean workbench. After removing the red blood cells, resuspend with PBS, count under the microscope, and adjust the cell density to 1×10 7 / mL, subcutaneously bearing tumors in the right anterior axillary position of BALB / c mice, injecting 200 μL each.

[0036] (2) After bearing the tumor, when the tumor tissue in the axilla of the mice grew to the size of a visible rice grain, the tumor volume was measured and the mice were randomly divided into 6 groups according to the tumor volume, which were model control group (A) and unsensitized DC Group (B), TCL-sensitized DC vaccine group (C), CURS+DC group (D), TCL+CURS-sensitized DC vaccine group (E) and TCL+LPS (lipopolysaccharide)-sensit...

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Abstract

The invention discloses application of sulfated curdlan as a dendritic cell vaccine adjuvant. The defect of applying a single dendritic cell vaccine is overcome. A dendritic cell vaccine with the sulfated curdlan as the adjuvant is obtained and then applied to immunotherapy of H22 liver cancer model mice, therefore, theoretical bases are provided for research of using polysaccharides as vaccine adjuvants, and a new therapeutic strategy is provided for clinical liver cancer immunotherapy. The dendritic cell vaccine is sensitized through tumor cell lysate in vitro and meanwhile is further subjected to immune activation through the sulfated curdlan, so that the antigen presentation capability of dendritic cells is improved; after the mice are inoculated, by highly expressing MHCI and MHCII inthe bodies of the mice, CD8+T cells are made to infiltrate into tumor tissue and play a role of killing tumor cells.

Description

technical field [0001] The present invention relates to the application of a kind of sulfated candlan polysaccharide. It belongs to the field of medical technology. Background technique [0002] Curdlan polysaccharide (curdlan) is an exopolysaccharide fermented by Alcaligenes faecalis, which has immunomodulatory, anti-tumor, anti-inflammatory, anti-coagulant and other activities. However, because it is difficult to dissolve in most organic solvents such as water and ethanol, the research and application of in vivo activity are limited. Sulfated curdlan polysaccharide (curdlan sulfate, CURS), a product obtained by sulfation modification of curdlan, greatly improves the solubility of curdlan. In vitro experiments found that CURS can activate mouse bone marrow derived dedritic cells (BMDCs) and mouse macrophage RAW264.7 cells; in vivo, it is used as an adjuvant for hepatitis B vaccine inoculation in BALB / c mice, It can enhance the immunogenicity of hepatitis B vaccine, incre...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/00A61P37/04A61P35/00
CPCA61K39/39A61K39/0011A61P37/04A61P35/00A61K2039/55583
Inventor 李平利王凤山金毅明张舒
Owner SHANDONG UNIV QILU HOSPITAL
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