Anti-CD3 nanobody CD3/Nb29 and preparation method and application thereof

A nanobody and inhibitor technology, which is applied to the anti-CD3 nanobody CD3/Nb29 and its preparation and application fields, can solve the problems of easy contamination, limited application scope, and difficulty in large-scale production.
CN110218256AActive Publication Date: 2019-09-10GUANGXI MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGXI MEDICAL UNIVERSITY
Publication Date
2019-09-10

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Abstract

The invention discloses an anti-CD3 nanobody CD3 / Nb29 and a preparation method and an application thereof. The nanobody provided by the invention comprises a determinant complementary area and a framework area; the determinant complementary area of the nanobody is composed of CDR1, CDR2 and CDR3; and through a nucleotide sequence and a host cell of the nanobody disclosed by the invention, the nanobody can be highly expressed in Escherichia coli, is applied to research and development of a CD3 molecular detection reagent, and is used for preparation of tumor inhibitors or tumor cell inhibitorsand preparation of drugs for inhibiting CD3 activity and promoting T cell proliferation.
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Description

technical field

[0001] The invention relates to anti-CD3 nanobody CD3 / Nb29 in the field of biomedicine and its preparation method and application. Background technique

[0002] CD3 molecule is an important differentiation antigen on the T cell membrane and a characteristic mark of mature T cells. It forms a complex with the T cell surface receptor TCR and plays an important role in intracellular signal transduction. CD3 antibody can specifically recognize CD3 molecules on the surface of T cells, causing cross-linking of T cell TCR-CD3 complexes, directly generating activation signals, leading to activation and proliferation of T cells. Current research shows that CD3 antibody plays an important role in tumor treatment.

[0003] However, there are many problems in the application of antibody drugs, such as long research and development cycle, high production cost, difficulty in large-scale production, poor stability and easy degradation, high storage cost, easy contaminatio...

Claims

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