Target exosome and preparation method and application thereof, drug delivery system and drug

Abstract

The invention provides a preparation method and application of a target exosome which can effectively improve the modifying efficiency of an exosome so as to realize large-scale preparation, a drug delivery system and a drug. The preparation method of the target exosome comprises the following steps S1, performing sulfhydrylation treatment on a target antibody so as to obtain a sulfhydrylation target antibody; and S2, enabling the sulfhydrylation target antibody to be connected with the exosome through a protein cross-linking agent. The target antibody is subjected to sulfhydrylation modification, so that an amino on the antibody is converted into a sulfydryl; and then the exosome and the antibody are in cross-linking combination so that the target exosome is obtained. Compared with a method of obtaining the target exosome through modification in a plasmid transfection manner in the prior art, the preparation method provided by the invention has the advantages that the problems that the transfection efficiency is low in the plasmid process and large-scale preparation and secretion of mother cells through genetic engineering plasmid transfection are difficult to perform can be solved, industrialization preparation of the target exosome can be realized, and the preparation method has important significance in research and application of the exosome as a delivery carrier and a drug carrier.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a targeted exosome, its preparation method, application, drug delivery system and drug. Background technique [0002] Tumor metastasis is an important cause of tumor death. Tumor metastasis refers to the infiltration of tumor cells from the primary site to surrounding tissues, and the spread to other tissues and organs of the body through the lymphatic system and blood system. This spread increases the mortality of patients. One of the reasons why tumor metastasis is clinically difficult to cure is that it is difficult for drugs to reach the metastatic site. According to the tumor-specific microenvironment and the difference between tumor tissue and normal tissue, researchers have designed a variety of nano-drug delivery systems to inhibit tumor metastasis. For example, loading P-3FaxNeu5Ac into PLGA nanoparticles, targeting sialooligosaccharide nano-drug delivery system, ...

AI Technical Summary

Problems solved by technology

These nano-drug delivery systems have alleviated the lack of anti-tumor metastasis preparations to a certain extent, but there are still some shortcomings: existing nano-preparations often use a variety of polymer materials, although these polymer materials are good drugs to some extent carrier, but its non-biological origin and defects in biocompatibility and safety have also become important factors that limit its entry into the clinic

Using this method to prepare targeting peptides has the problem of low transfection efficiency, making it difficult to achieve large-scale production

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