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A method for extracting quinoa polypeptide by alkali extraction-membrane method

A kind of quinoa, membrane method

Active Publication Date: 2021-07-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the existing quinoa protein extraction methods are mainly alkaline extraction, acid precipitation and enzymatic separation. However, these methods take a long time, the extraction rate is very low, and the purity of the peptide is not high in oxidation resistance.
Patent CN109182434A discloses a preparation method of quinoa protein polypeptide hydrolyzate with weight loss activity, and specifically discloses the use of alkali extraction and acid precipitation method to extract protein, and then enzyme treatment to extract quinoa peptide, but acid precipitation will not only lose a lot Protein components, resulting in low yield of peptides, and also produce a large amount of acid and alkali to pollute the environment

Method used

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  • A method for extracting quinoa polypeptide by alkali extraction-membrane method
  • A method for extracting quinoa polypeptide by alkali extraction-membrane method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Amylase treatment and alkaline extraction of quinoa protein

[0035] Wash 4kg of quinoa, beat through a 100-mesh sieve, add water until the ratio of solid to liquid is 1:12, use 50°C, pH 6.3, add 16g of α-amylase for 2 hours of enzymatic hydrolysis, and then adjust the pH to 11, Carry out leaching for 3 hours, after centrifugation, pass the supernatant through the ceramic membrane, the membrane flux is 60-110L / m 2 h, the protein extraction rate is 90%.

[0036] (2) The first step of ultrafiltration membrane treatment

[0037] The ceramic membrane clarified liquid is processed through a 5000 ultrafiltration membrane, and the protein retention rate is 76.4%.

[0038] (3) Protease hydrolysis

[0039] Adjust the pH of the 5000 ultrafiltration membrane retentate to 10, heat to 50°C, add 7.33g of alkaline protease, and use 90°C for 10min to inactivate the enzyme after 3 hours of enzymatic hydrolysis.

[0040] (4) The second step of ultrafiltration membrane treatment ...

Embodiment 2

[0049] (1) Amylase treatment and alkaline extraction of quinoa protein

[0050] Wash 4kg of quinoa, beat through a 100-mesh sieve, add water until the ratio of solid to liquid is 1:10, use 50°C, pH7, add 16g of α-amylase for 2 hours of enzymolysis, then adjust the pH to 10, and carry out 2 hours Hours of leaching, after centrifugation, the supernatant is passed through the ceramic membrane, and the membrane flux is 60-110L / m 2 h, the protein extraction rate is 80%.

[0051] (2) The first step of ultrafiltration membrane treatment

[0052] The ceramic membrane clarified liquid is processed through a 5000 ultrafiltration membrane, and the protein retention rate is 76.4%.

[0053] (3) Protease hydrolysis

[0054] Adjust the pH of the 5000 ultrafiltration membrane retentate to 10, heat to 50°C, add 7.33g of alkaline protease, and use 90°C for 10min to inactivate the enzyme after 3 hours of enzymatic hydrolysis.

[0055] (4) The second step of ultrafiltration membrane treatment...

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Abstract

The invention discloses a method for extracting quinoa polypeptide by alkali extraction-membrane method, which belongs to the field of food processing. The invention adopts amylase to assist alkaline extraction, and after the first ultrafiltration, protease is used for enzymolysis, and the quinoa polypeptide is obtained through the second ultrafiltration. By adopting the method of the present invention, the polypeptide yield is as high as 39.2%, the degree of hydrolysis reaches 18.5%, and the molecular weight is small. The molecular weight of 99.57% of the obtained polypeptide molecules is less than 2500, which is easy to be absorbed by the human body, has a high protein extraction rate, and has in vitro antioxidant activity; Amylase-assisted alkaline extraction effectively improves the protein extraction rate, not only avoids the large amount of acid used in the traditional alkaline extraction and acid precipitation method, but also retains the protein lost in the acid precipitation step.

Description

technical field [0001] The invention relates to an alkali extraction-membrane method for extracting quinoa polypeptide, which belongs to the field of food processing. Background technique [0002] With the development of social economy, the improvement of productivity and the improvement of people's living standards, people's demand for healthy food is increasingly urgent. Due to the long-term irregular life and unhealthy diet of contemporary people, the digestion and absorption ability is reduced. The staple food that is often eaten is rich in carbohydrates, but lacks high-quality protein. Therefore, there is an urgent need for a food that is easy to absorb and rich in essential amino acids. . [0003] Quinoa is the most suitable "whole nutritional food" recognized by the Food and Agriculture Organization of the United Nations. It is called the lost ancient "nutrition gold", "super grain" and "future food" by international nutritionists. Respected as the "King of Vegetari...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC07K1/34C12P21/06
Inventor 杨严俊常翠华唐子箫苏宇杰顾璐萍李俊华
Owner JIANGNAN UNIV