Method for extracting micro plastics from human and animal excreta

A technology for human and animal excrement and microplastics, applied in chemical instruments and methods, sampling, analysis materials, etc., can solve the problems of severe digestion conditions, inability to separate microplastics, and large damage to microplastics, improving efficiency and facilitating accurate analysis. , the effect of high-fidelity extraction

Active Publication Date: 2019-09-17
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing methods for extracting microplastics from biological tissues and sludge are not suitable for fecal samples, and mainly have the following deficiencies: (1) Density separation is not suitable for separating fecal digestion liquid and microplastics; (2) The digested fecal residues will Covering the surface of microplastics will affect the identification of microplastics by microscope-Raman spectroscopy of subsequent microplastics; (3) The existing digestion conditions are too intense, which will cause great damage to microplastics, which is not conducive to true and accurate evaluation Health risks of microplastics
[0004] Therefore, when studying the accumulation and excretion of microplastics in vivo and the health risk assessment of microplastics, the existing methods have a great influence on the properties of microplastics themselves, and cannot efficiently separate microplastics from feces. A fast, convenient, high-fidelity method to extract microplastics from feces

Method used

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  • Method for extracting micro plastics from human and animal excreta
  • Method for extracting micro plastics from human and animal excreta
  • Method for extracting micro plastics from human and animal excreta

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Feces freeze-drying: freeze-dry the collected human feces in a freeze-drying apparatus, and take 5 g dry weight of the freeze-dried sample, and 3 parallel samples.

[0046] (2) Sample digestion: transfer the freeze-dried stool in step (1) to a 2L beaker, and add 20g / L FeSO to it in turn 4 ·7H 2 O catalyst and 30% H 2 O 2 1:2.5, fully contact the reaction under stirring, until no bubbles are generated, according to 20g / L FeSO 4 ·7H 2 O catalyst and 30% H 2 O 2 The volume ratio of 1:2.5 continues to add Fenton reagent, adding 30% H each time 2 O 2 Do not exceed 50 mL until 500 mL 30% H is added 2 O 2 And the reaction is completed, and the temperature is controlled not to exceed 40°C by using an ice bath during the reaction. Use a circulating water vacuum pump to suction and filter the remaining organic matter onto the water-based CN-CA filter membrane. The number of water-based CN-CA filter membranes is 11, and the water-based CN-CA filter membrane after suction and filtra...

Embodiment 2

[0050] (1) Feces freeze-drying: freeze-dry the collected human feces in a freeze-drying apparatus, and take 2g dry weight of the freeze-dried sample, and 3 parallel samples.

[0051] (2) Transfer the freeze-dried feces in step (1) to a 2L beaker, and add to it in a volume ratio of 20g / LFeSO 4 ·7H 2 O catalyst and 30% H 2 O 2 1:2.5, fully contact the reaction under stirring, until no bubbles are generated, according to 20g / L FeSO 4 ·7H 2 O catalyst and 30% H 2 O 2 The volume ratio of 1:2.5 continues to add Fenton reagent, adding 30% H each time 2 O 2 Do not exceed 50 mL until 200 mL 30% H is added 2 O 2 And the reaction is completed, and the temperature is controlled not to exceed 40°C by using an ice bath during the reaction. Use a circulating water vacuum pump to suction and filter the remaining organic matter onto the water-based CN-CA filter membrane. The number of water-based CN-CA filters is 5, and transfer the water-based CN-CA filter membrane after suction to a 500 mL beaker...

Embodiment 3

[0055] (1) Feces freeze-drying: freeze-dry the fetched chicken excrement in a freeze dryer, take 5g dry weight of the freeze-dried sample, and 3 parallel samples.

[0056] (2) Transfer the freeze-dried feces in step (1) to a 2L beaker, and add to it in a volume ratio of 20g / LFeSO 4 ·7H 2 O catalyst and 30% H 2 O 2 1:2.5, fully contact the reaction under stirring, until no bubbles are generated, according to 20g / L FeSO 4 ·7H 2 O catalyst and 30% H 2 O 2 The volume ratio of 1:2.5 continues to add Fenton reagent, adding 30% H each time 2 O 2 Do not exceed 50 mL until 500 mL 30% H is added 2 O 2 And the reaction is completed, and the temperature is controlled not to exceed 40°C by using an ice bath during the reaction. Use a circulating water vacuum pump to suction and filter the remaining organic matter onto the water-based CN-CA filter membrane. The number of water-based CN-CA filter membranes is 11, and the water-based CN-CA filter membrane after suction and filtration is transferre...

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Abstract

The invention discloses a method for extracting micro plastics from human and animal excreta, and belongs to field of environmental health risk assessment. The method comprises the steps of excrement freeze-drying, sample decomposition and sample extraction, wherein the decomposition process comprises the steps of treatment with a Fenton's reagent, decomposition of concentrated nitric acid and extraction of absolute ethyl alcohol. According to the method, the micro plastics can be extracted from human and animal excreta conveniently and quickly under the condition of guaranteeing minimized influence on the micro plastics, the types and abundance of the micro plastics in biological excreta are accurately analyzed, and powerful technical support and data support are provided for researching an enrichment and excretion rule of the micro plastics in vivo and health risk assessment.

Description

Technical field [0001] The invention belongs to the field of environmental health risk assessment, and specifically relates to a method for extracting microplastics from human and animal feces, which can quickly, efficiently, and high-fidelity extract microplastics from human and animal feces under the condition of minimizing the impact on microplastics , It is convenient to accurately analyze the types and abundance of microplastics in biological feces, and provide strong technical guarantee and data support for studying the accumulation and excretion of microplastics in organisms, as well as health risk assessment. Background technique [0002] Microplastics refer to plastic particles with a diameter of less than 5 mm. On the one hand, they come from industrial raw materials, personal care (such as toothpaste, facial cleanser) and the primary source of small particles of plastic microbeads in medical products; on the other hand, they can come from large particles in the environm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/34G01N1/40G01N15/00
CPCG01N1/28G01N1/34G01N1/4077G01N1/4044G01N15/00G01N2001/4088G01N21/65G01N33/4833B01D71/16B01D71/20B01D71/36G01N2001/4094
Inventor 张宴闫泽华任洪强
Owner NANJING UNIV
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