Fluid-based cell and microorganism treating and preserving reagent and preparation method thereof

Abstract

The invention discloses a fluid-based cell and microorganism treating and preserving reagent and a preparation method thereof. Halogenated cyanoacetamide, a moisturizing agent, a pH buffer agent, an osmotic pressure regulator, a surfactant, an antioxidant, pigment and essence are uniformly stirred, the obtained mixture is added to deionized water at the water temperature of 30-35 DEG C, and sodium hydroxide is added to adjust the pH value to 5.5-6.5 to obtain the fluid-based cell and microorganism treating and preserving reagent. The reagent is a fixing agent for fixing biological samples, contains no mercury or formaldehyde, can be compatible with different parasitism stages (such as sporocysts, eggs, larvae and trophozoites) and is effective under the condition that no polyvinyl alcohol or aldehyde (such as glyoxal and glutaraldehyde) is added.

Description

technical field [0001] The invention belongs to the technical field of medical detection, in particular to a fixative for fixing biological samples, in particular to a liquid-based reagent for treating and preserving microorganisms and a preparation method thereof. Background technique [0002] Various types of samples, especially the detection of formed elements such as cells and microorganisms in body fluid samples, require a certain pre-treatment process. This pre-treatment can increase the positive detection rate and reduce the impact of impurities in the sample on the formed elements. In the final test, in severe cases, the test will be invalid or impossible to carry out the test. It can be seen that correct pre-processing for the detection of cells and microorganisms in body fluid samples is of great significance to the accuracy and reliability of test results. Generally speaking, in order to effectively detect cells or microorganisms, the samples need to be preserved,...

AI Technical Summary

Problems solved by technology

[0003] While parasitic infections have a long history of causing disease in humans and animals, the diagnosis of intestinal parasites is usually confirmed by staining and microscopic identification of helminth eggs and larvae or protozoan trophozoites and / or cysts in stool samples, but regardless of the However, one problem with diagnosing parasitic infections is the delay between collection and examination of the sample. If there is no suitable fixative, it will cause the sample to grow bacteria due to some special enzymes it contains or the environment in which the sample exists. rapid sample degradation due to microorganisms and, if not processed in a timely manner, can interfere with accurate diagnosis of intestinal parasites, which depends on obtaining optimal fixation of the sample at the time of collection, therefore, fixative solutions are often used to process samples for Diagnosis of parasites

[0004] Currently, fixatives used to preserve stool specimens often contain mercury, formaldehyde, or formalin, which have a number of disadvantages that limit their use

For example, mercury-based fixatives and Schaudinn's fixative containing mercuric chloride base, while providing good definition, present disposal problems due to high cost and scarcity of disposal companies willing to dispose of mercury waste, and the fixative contains Preservatives such as formaldehyde or formalin can cause similar environmental and health concerns

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