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Extracting method and application of croton crassifolius root extract

An extraction method and extraction technology, applied in the field of extraction of chicken bone vetch root extract, can solve the problems of less research and achieve the effects of high anti-tumor activity, huge social benefits, and easy industrialization

Pending Publication Date: 2019-10-01
INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, it is reported that the low polar part of the herb is rich in active compounds, but there are few specific studies

Method used

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  • Extracting method and application of croton crassifolius root extract
  • Extracting method and application of croton crassifolius root extract
  • Extracting method and application of croton crassifolius root extract

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Embodiment 1

[0034] (1) Materials and methods

[0035] Crassifolius The roots were purchased at a Chinese herbal medicine market in Bozhou, Anhui, China, in October 2017. This species was identified by Prof. Shan Chenggang, Institute of Agricultural Food Science and Technology, Shandong Academy of Agricultural Sciences (IAST, SAAS), Jinan, China. Voucher samples (No.17-10-29) are kept at the IAST Bioactive Substances and Functional Foods Laboratory at SAAS.

[0036] (2) Extraction process:

[0037] (1) Take the dried root of Jiguxiang, pulverize it with a traditional Chinese medicine grinder, and pass it through a 40-mesh sieve (450 μm). Weigh 2.0 kg of sample and fill it with supercritical CO in 10 times 2 In the reaction vessel (650 mL) of the fluid extractor, 200 g each time. At 25 MPa, 35 o C under static extraction for 30 minutes, followed by dynamic extraction for 30 minutes (CO 2 Flow rate 5.0 L / min), with methanol as entrainer (flow rate 5.4 mL / min). A total of 100.4 g of ...

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Abstract

The invention belongs to the technical field of drug extracting, and particularly relates to an extracting method and application of croton crassifolius root extract. The extracting method specifically comprises the following steps of (1) selecting the dried root of croton crassifolius, crushing, screening, weighing a sample, extracting by supercritical fluid, obtaining deep yellow extract cream,dispersing the extract cream by warm water, and fully extracting by dichloromethane, so as to obtain the extract; (2) preparing an elution agent for eluting, performing thin-layer chromatography analysis on the extract, selecting a petroleum ester-acetone separation system, and roughly separating; combining with thin-layer chromatography detection, and obtaining five components A1 to A5; (3) eluting the component A2 in a gradient way, combining with the thin-layer chromatography detection, and obtaining three parts A2a, A2b and A2c; fining the part A2b by a silica gel column, so as to obtain the croton crassifolius root extract. The croton crassifolius root extract has the advantages that the purity is high, the anti-tumor activity is high, the proliferation-resisting and cell withering-inducing functions are realized, and the potential as the anti-cancer candidate drug is realized.

Description

technical field [0001] The invention belongs to the technical field of medicine extraction, and in particular relates to an extraction method and application of a chicken bone radix extract. Background technique [0002] Croton is a genus of Euphorbiaceae, with more than 1300 species in the world, widely distributed in tropical and subtropical regions. About 21 species of Croton grow in China, some of which have long been used to relieve dysmenorrhea and Chinese medicine for indigestion and malaria. Jiguxiang is a species of Croton genus, which is famous for containing a variety of terpenoids, which have a wide range of biological activities. Additionally, it contains alkaloids, flavanones, lignans, polyphenolic compounds, phenylbutyric acid and peptides. [0003] Croton crassifolius Geisel is a type of chicken bone incense, which is mainly distributed in southern China and other parts of Southeast Asia. As a Chinese medicinal herb called "Ji Gu Xiang", its root has bee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/54A61K36/47A61P35/00A61K125/00
CPCC07D307/54A61K36/47A61P35/00Y02P20/54
Inventor 刘超孙金月陈英凯张瑞瑞王青郭溆
Owner INST OF AGRO FOOD SCI & TECH SHANDONG ACAD OF AGRI SCI
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