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Preparation method of lipid rich in sphingomyelin

A sphingomyelin and lipid technology, applied in the field of lipid preparation, can solve problems such as inability to be widely used in food, and achieve the effects of ensuring food safety and improving enrichment effect

Inactive Publication Date: 2019-10-01
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the cases of extracting sphingomyelin based on dairy products use toxic organic solvents such as chloroform, which cannot be widely used in food

Method used

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  • Preparation method of lipid rich in sphingomyelin
  • Preparation method of lipid rich in sphingomyelin
  • Preparation method of lipid rich in sphingomyelin

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preparation example Construction

[0014] In a specific embodiment of the present invention, there is provided a method for preparing lipids rich in sphingomyelin, the preparation method comprising the following steps:

[0015] In order to solve the above-mentioned technical problems, the present invention provides a method for preparing sphingomyelin-rich lipids, the preparation method comprising the following steps:

[0016] Step 1): Heat the ethanol solution to 55°C to 80°C. After the temperature is stable, use a constant flow pump to inject the dairy products into the ethanol solution at a constant speed through a 1ml needle at a constant speed, wherein the concentration of the ethanol solution is 50%. ~80%, the volume ratio of dairy product to ethanol solution is 1:4~1:6, and the speed of the constant flow pump is 5rpm~15rpm.

[0017] Step 2): centrifuge the reaction product obtained in step 1) at a speed of 7500-9000 rpm / s in a centrifuge, and collect the supernatant;

[0018] Step 3): drying and concent...

Embodiment 1

[0021] The method for preparing the lipid rich in sphingomyelin of embodiment 1 comprises the following steps:

[0022] Step 1): Heat the ethanol solution to 80°C. After the temperature is stable, use a constant flow pump to inject the skimmed milk powder solution through a 1ml needle into the ethanol solution at a constant speed at a constant speed, and react for 30 minutes. The concentration of the ethanol solution is 50 %, the volume ratio of skimmed milk powder solution to ethanol solution is 1:6, and the constant flow pump speed is 5rpm;

[0023] Step 2): centrifuge the reaction product obtained in step 1) at 7500rpm / s for 30min in a centrifuge, and collect the supernatant;

[0024] Step 3): Transfer the upper layer liquid collected in step 2) to a rotary evaporator, and evaporate to dryness at a water temperature of 55°C. After the upper layer liquid is evaporated to 1ml, collect the concentrated upper layer liquid; transfer the obtained concentrated upper layer liquid t...

Embodiment 2

[0029] The method for preparing the lipid rich in sphingomyelin of embodiment 2 comprises the following steps:

[0030] Step 1): Heat the ethanol solution to 55° C., and after the temperature stabilizes, inject milk into the ethanol solution through a 1ml needle at a constant speed with a constant speed pump, and react for 30 minutes, wherein the concentration of the ethanol solution is 60%. The volume ratio of milk volume to ethanol solution is 1:4, and the constant flow pump speed is 15rpm;

[0031] Step 2): centrifuge the reaction product obtained in step 1) at a speed of 9000 rpm / s for 30 minutes in a centrifuge, and collect the supernatant;

[0032] Step 3): Transfer the upper layer liquid collected in step 2) to a rotary evaporator, and evaporate to dryness at a water temperature of 55°C. After the upper layer liquid is evaporated to 1ml, collect the concentrated upper layer liquid; transfer the obtained concentrated upper layer liquid to vacuum drying box, vacuum-dried...

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Abstract

The invention relates to a preparation method of lipid rich in sphingomyelin. The method adopts specific a dairy product type, solvent temperature and a dairy product to extractant ratio, and can obviously improve the enrichment effect of the sphingomyelin in the lipid, and the selection of extractant ethanol solution can ensure food safety.

Description

technical field [0001] The invention relates to a preparation method of lipid rich in sphingomyelin, which belongs to the technical field of oil processing. Background technique [0002] Sphingomyelin (sphingomyelin) is a sphingolipid composed of phosphorylcholine (or phosphoethanolamine) connected to the C-1 hydroxyl group of ceramide. It is a kind of bioactive lipid in human milk. It is composed of sphingosine, Composed of fatty acids, phosphoric acid and nitrogenous bases. Sphingomyelin is the most abundant phospholipid in human milk fat, accounting for 29.7%-45.5% of the total phospholipids. It has a high proportion in the milk fat globule membrane, has many benefits to human health, and is a very important component of milk phospholipids. Supplementation with sphingomyelin has been reported to prevent disease and maintain good health. Phospholipids and sphingomyelin play an important role in infant nutrition. As the nutritional carrier of long-chain polyunsaturated f...

Claims

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Application Information

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IPC IPC(8): C07F9/113
CPCC07F9/113
Inventor 涂凌云张绫芷王智浩张蔚筱武玉洁牟柏林江晨瑜杨文卿程川川
Owner NINGBO UNIV
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