Isolated carp antiviral protein Rhbdd3 and antiviral activity thereof

An anti-virus and protein technology, applied in the direction of anti-viral agents, peptide/protein components, medical preparations containing active ingredients, etc., can solve problems such as shortage, and achieve the effect of reducing infectivity

Active Publication Date: 2019-10-01
上海市水产研究所(上海市水产技术推广站)
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  • Abstract
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Problems solved by technology

At present, there is still a lack of drugs and methods to effectively control SVC. The internationally accepted control measures are to monitor SVCV, detect it early, isolate and kill it

Method used

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  • Isolated carp antiviral protein Rhbdd3 and antiviral activity thereof
  • Isolated carp antiviral protein Rhbdd3 and antiviral activity thereof
  • Isolated carp antiviral protein Rhbdd3 and antiviral activity thereof

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Embodiment Construction

[0042] The following describes several preferred embodiments of the present invention with reference to the accompanying drawings, so as to make the technical content clearer and easier to understand. The present invention can be embodied in many different forms of embodiments, and the protection scope of the present invention is not limited to the embodiments mentioned herein.

[0043] (1) Extraction of carp RNA

[0044] Total RNA was extracted by the classic Trizol method. Cut carp tissue pieces with ophthalmic scissors and put them directly into a mortar, add a small amount of liquid nitrogen, grind quickly, transfer to a centrifuge tube after grinding fully, take 50-100mg of tissue, add 1mL Trizol, mix well, place at room temperature for 10min, and then Centrifuge at 12000×g for 5 minutes at 4°C.

[0045] Add 200 μL of chloroform to the supernatant, vortex and mix well, place at room temperature for 15 min, centrifuge at 12000×g for 15 min at 4 °C.

[0046] Take the upp...

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Abstract

The invention relates to the technical field of fish genetic engineering, and discloses an isolated carp antiviral protein Rhbdd3 and antiviral activity thereof. The nucleotide sequence of a carp antiviral protein Rhbdd3 encoding gene is shown in 1-1050bp in SEQ ID NO:1, and the amino acid sequence encoded by the carp antiviral protein Rhbdd3 encoding gene is shown in SEQ ID NO:2. The expression of the cloned Rhbdd3 protein not only can inhibit the proliferation of a spring viremia of carp virus (SVCV), but also can inhibit the proliferation of an infectious pancreatic necrosis virus (IPNV). The Rhbdd3 protein plays an important role when cells defend against the infection of the SVCV and IPNV, and does not affect the normal activity of the cells. The gene and the protein provide a new target for preparing drugs resisting the SVCV and the IPNV, and also provide an important technical basis for breeding novel fish anti-virus varieties.

Description

technical field [0001] The invention relates to the technical field of genetic engineering of freshwater fish, in particular to an isolated carp antiviral protein Rhbdd3 and its antiviral activity. Background technique [0002] With the continuous development of my country's aquaculture industry, the incidence of aquatic animal diseases, especially viral diseases, has shown a clear upward trend in recent years. Aquatic animal viral diseases have rapid onset and high mortality. Most of them have no effective vaccines or targeted drugs. Therefore, the development of broad-spectrum antiviral drugs and the cultivation of new antiviral species of fish have very important practical value. [0003] Innate immunity is the body's first line of defense against virus invasion. The latest research has found that Rhomboid family member Rhbdd3 (Rhomboid domain-containing protein 3) plays an important role in mammalian innate immunity. The Rhomboid family was first discovered in Drosophi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K14/46C12N15/66C12N15/85A01K67/027A61K38/17A61P31/14
CPCC07K14/461C12N15/66C12N15/85A01K67/0276A61P31/14A01K2227/40A01K2267/02A61K38/00Y02A50/30Y02A40/81
Inventor 邵玲汤茜肖雨
Owner 上海市水产研究所(上海市水产技术推广站)
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