A mouse embryo transplantation preservation tool and its manufacturing method

A technology of mouse embryos and manufacturing methods, applied in the preservation of human or animal bodies, medical science, animal delivery, etc., to achieve the effects of avoiding the decline in embryonic development rate, rapid operation, and reducing time

Active Publication Date: 2021-08-06
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is the problem of temporary preservation of embryos in a low-temperature and low-humidity environment outside the body during transplantation

Method used

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  • A mouse embryo transplantation preservation tool and its manufacturing method
  • A mouse embryo transplantation preservation tool and its manufacturing method
  • A mouse embryo transplantation preservation tool and its manufacturing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]This embodiment is the mouse embryo transplantation and preservation tool described in the present invention, comprising Pasteur tube 1, centrifuge tube 2, syringe 3 and rubber piston 4, the specific structure and connection relationship are: the rubber piston 4 is inserted from the tail The jacket of the syringe 3, cut off a part of the volume of the cone of the syringe jacket appropriately, so that the width of the cone mouth is equal to the width of the outer diameter of the Pasteur tube 1, properly cut off the two wings of the jacket of the syringe 3, so that the length of the two wings is equal to or slightly greater than the diameter of the centrifuge tube 2, Insert the jacket of the syringe 3 into the centrifuge tube 2, fix the jacket so that the jacket cannot move up and down in the centrifuge tube 2, and the Pasteur tube 1 is fixed on the cone of the jacket.

[0036] The centrifuge tube 2 is a commonly used 15mL plastic centrifuge tube with a diameter of 1.75cm, ...

Embodiment 2

[0041] like Figure 1 to Figure 6 As shown, the present embodiment is a manufacturing method of the mouse embryo transplantation preservation device according to the present invention, comprising the following steps:

[0042] (1) Take a new sterile 15mL centrifuge tube 2 without pyrogen, add 10.5mL autoclaved water to the centrifuge tube and perform autoclaving;

[0043] (2) Take a new sterile 1mL syringe 3 without pyrogen, discard the needle, take out the core rod, remove the rubber piston, and then insert the rubber piston 4 into the jacket from the tail;

[0044] (3) Use scissors to cut off a part of the cone head of the jacket to widen the mouth of the cone head with a width of 0.6-0.8 cm. After cutting, use Pasteur tube 1 to try to fix it so that the graft tube can not be fixed up and down. The left and right movement shall prevail, and the cone head of such a width can fix the Pasteur tube;

[0045] (4) Properly cut off the two wings of the jacket of the syringe 3 so t...

Embodiment 3

[0051] This example is an example of the in vitro preservation effect of using the mouse embryo transplantation preservation tool of the present invention.

[0052] 1. Add 0.2mL of normal saline, PBS or transplantation liquid to the syringe jacket, and add transplantation fluid to the jacket;

[0053] 2. The three-stage method of the Pasteur tube absorbs 15-20 fertilized eggs, inserts the Pasteur tube with the embryos into the cone mouth of the jacket, and keeps it vertical until it is inserted into the jacket under the liquid surface, about 0.5 below the liquid surface. about mm. Finally, put the whole device into a water bath at 39°C to heat it in a water bath, store it in vitro for about 20 minutes, then take out the Pasteur tube with fertilized eggs, blow all the embryos out and transfer them to a culture dish for continuous culture. The implementation method of the comparative example in the present invention is that the Pasteur tube three-stage method draws 15-20 fertil...

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Abstract

A tool for mouse embryo transplantation preservation and its manufacturing method, comprising a centrifuge tube, a syringe, a rubber piston and a Pasteur tube, the rubber piston is inserted into the syringe jacket from the tail, and a part of the volume of the cone head of the syringe jacket is appropriately cut off to make the cone The width of the mouth is equal to the width of the outer diameter of the Pasteur tube. Properly cut off the two wings of the syringe jacket so that the length of the two wings is equal to or slightly greater than the diameter of the centrifuge tube. Insert the syringe jacket into the centrifuge tube and fix the jacket so that the jacket cannot be left or right in the centrifuge tube. Moving up and down, the Pasteur tube is fixed to the cone of the jacket. By adopting the invention, the transplanted embryos can be effectively preserved in the external environment, shortening the low temperature and low humidity time of the embryos in vitro, and facilitating the implantation and later development of the embryos.

Description

technical field [0001] The invention relates to the technical field of mouse embryo transplantation and preservation instruments, in particular to a mouse embryo transplantation and preservation instrument and a manufacturing method thereof. Background technique [0002] Embryo transfer plays a crucial role in the success of transgenic cloned animals. Embryo transfer requires recipient female mice of suitable estrus, and early embryo transfer to different parts of the mouse will lead to different pregnancy outcomes. During the process of taking out the embryo from the incubator and transplanting it into the fallopian tube or uterus of the recipient female mouse, it will be stimulated by the external low temperature and low humidity environment, which will greatly affect the development efficiency of the embryo and the final pregnancy result. The key to the success of embryo transfer lies in the recipient mice in suitable estrous period, the appropriate transplantation site ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61B17/435A61D19/04A01N1/02
CPCA01N1/0263A61D19/04
Inventor 孙俊铭何敏欧阳轶强张名媛郭晓萍莫忠香李家富刘佳娟胡冰何萍
Owner GUANGXI MEDICAL UNIVERSITY
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