A microbial bacteria and its application containing ethanol fake silk yeast
A technology of microbial agent and Candida, which is applied in the direction of microorganisms, methods based on microorganisms, and the treatment of biological organic parts, etc., can solve problems such as no clear standards, achieve good application effects, facilitate large-scale use, and have strong versatility Effect
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Embodiment 1
[0028] A microbial inoculum containing Candida ethanolic yeast, its preparation method comprises the steps:
[0029] (1) fully dissolving the premixed solid fermentation medium with water in the fermenter;
[0030] (2) adding 0.1‰ of pre-compounded solid microbial compound bacterial agent (based on the pre-mixed solid fermentation medium solution after water dissolving) to the medium solution obtained in step (1), adjusting with sodium hydroxide pH6.5, stir well and seal the container with a lid;
[0031] (3) Fermentation at 30°C for 6 days, then adding 20wt% corn biochemical fulvic acid, and leaving it for 1 day to prepare the microbial inoculum containing Candida ethanolica; its pH is 3.8, and the total number of bacteria is 2.1 ×10 7 cfu / mL, of which Candida ethanolica 2 × 10 5 cfu / mL.
[0032] After the described premixed solid fermentation medium is fully dissolved with water, the mass percent of each raw material is as shown in Table 1:
[0033] Table 1
[0034] ...
Embodiment 2
[0041] A microbial inoculum containing Candida ethanolic yeast, its preparation method comprises the steps:
[0042] (1) fully dissolving the premixed solid fermentation medium with water in the fermenter;
[0043] (2) adding 1‰ of the pre-compounded solid microbial compound bacterial agent (based on the pre-mixed solid fermentation medium solution after water dissolving) to the medium solution obtained in step (1), adjusting with sodium hydroxide pH 5, stir well and seal the container with a lid;
[0044] (3) Fermentation at 40°C for 5 days, then adding 25wt% corn biochemical fulvic acid, and leaving it for 1 day to prepare the microbial inoculum containing Candida ethanolica; its pH is 3.5, and the total number of bacteria is 2.6 ×10 7 cfu / mL, of which Candida ethanolica 6×10 5 cfu / mL.
[0045] After the described premixed solid fermentation medium is fully dissolved with water, the mass percent of each raw material is as shown in Table 3:
[0046] table 3
[0047] ...
Embodiment 3
[0053] A microbial inoculum containing Candida ethanolic yeast, its preparation method comprises the steps:
[0054] (1) fully dissolving the premixed solid fermentation medium with water in the fermenter;
[0055] (2) adding 0.5‰ of pre-compounded solid microbial compound bacterial agent (based on the pre-mixed solid fermentation medium solution after water dissolving) to the medium solution obtained in step (1), adjusting with sodium hydroxide pH8, stir well and seal the container with a lid;
[0056] (3) Fermentation at 25°C for 9 days, then adding 30wt% corn biochemical fulvic acid, and leaving for 1 day to prepare the microbial inoculum containing Candida ethanolicus; its pH is 3.5, and the total number of bacteria is 2.4 ×10 7 cfu / mL, of which Candida ethanolica 4 × 10 5 cfu / mL.
[0057]After the described premixed solid fermentation medium is fully dissolved with water, the mass percent of each raw material is as shown in Table 5:
[0058] table 5
[0059]
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