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Sports genetic marker site detecting method based on nucleic acid mass spectrometry typing technology and product thereof

A nucleic acid mass spectrometry and genetic marker technology, applied in the field of exercise potential gene detection, can solve problems such as one-sided detection results, and achieve the effects of reducing detection costs, high throughput, and avoiding experimental operations.

Pending Publication Date: 2019-10-22
沈阳哥瑞科技有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the existing detection schemes, most of them only detect a single polymorphic site for the evaluation of an exercise index, and most of the existing detection products use a combination of a small number of polymorphic sites to evaluate the comprehensive exercise of the subject Ability, this test result is relatively one-sided

Method used

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  • Sports genetic marker site detecting method based on nucleic acid mass spectrometry typing technology and product thereof
  • Sports genetic marker site detecting method based on nucleic acid mass spectrometry typing technology and product thereof
  • Sports genetic marker site detecting method based on nucleic acid mass spectrometry typing technology and product thereof

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Embodiment 1

[0065] see Figure 1-3 , the present invention provides the following technical solutions: a method for detecting sports genetic marker sites based on nucleic acid mass spectrometry technology, including a sports potential evaluation system, the detection method is to obtain sports gene genetic markers through text and data mining, and determine site weights, And establish a motor potential assessment system, according to the specific sequence information of the site, design the amplification primers containing the DNA fragment of the site information and the extension primers for the genotype detection of the site, and perform multiple PCR amplification and genetic analysis on the DNA of the subject. The single base extension reaction of the site, the extension product is detected by nucleic acid mass spectrometry, the specific genotype of the polymorphic site is determined, the genotype is evaluated according to the motor potential evaluation system, and the statistical analy...

Embodiment 2

[0086] see Figure 1-18 .

[0087] 1. Obtain the DNA of the subject (Liu)

[0088] (1) Obtain a sample of Liu's oral mucosal epithelial cells using oral swabs;

[0089] (2) Total DNA was extracted from Liu's oral mucosal epithelial cell sample by a conventional DNA extraction kit to obtain a DNA solution.

[0090] 2. Amplification primer grouping:

[0091]第一组:rs1803285、rs2010963、rs1800012、rs11549465、rs970547、rs8192678、rs1049434、rs1815739、rs5418、rs10768683、rs12722、rs7181866、rs8031031、rs4253778、rs8111989、rs1800795、rs1801282、rs699、rs4697425、rs2070744、rs12594956、rs3804358、rs1801131、 rs41274853, rs3763679, rs1042713, rs17602729;

[0092] 第二组:rs6420424、rs182549、rs429358、rs3736228、rs5219、rs5443、rs9366637、rs2298585、rs33972313、rs1544410、rs855791、rs1111875、rs12785878、rs1800592、rs7903146、rs7412、rs2282679、rs6552828、rs2108622、rs1801282、rs71404070、rs4988235、rs10741657、 rs4654748, rs6564851.

[0093] 3. Multiplex PCR reaction:

[0094] (1) According to the grouping in step 2, the ampl...

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Abstract

The invention belongs to the technical field of sports potential gene detection, and particularly relates to a sports genetic marker site detecting method based on the nucleic acid mass spectrometry typing technology and a product thereof. A sports potential evaluating system is involved. The detecting method includes the steps of conducting text and data mining to obtain sports genetic markers, determining the weights of sites, and analyzing and putting away high-reliability sports ability genetic markers through text and data mining and experimental verifying so that the genetic markers canbe more comprehensive and reliable. By using a multiplex PCR and the nucleic acid mass spectrometry in cooperation, the complicated experimental operations are avoided, and high-flux detection is realized; higher flux is realized compared with a conventional PCR and a fluorescent quantitative PCR; normally, when 52 genetic sites are detected, the PCR reaction need to be conducted 52 times; all thesites can be detected through the method only by conducting two rounds of twice PCR reactions; compared with second-generation sequencing, the detection cost and data analysis complexity are reduced,and the method has higher advantages for detecting the same number of genetic markers.

Description

technical field [0001] The invention belongs to the technical field of athletic potential gene detection, and in particular relates to a method for detecting athletic genetic marker sites and products thereof based on nucleic acid mass spectrometry typing technology. Background technique [0002] With the continuous development of scientific research, after the completion of the Human Genome Project and the full development of the Post-Genome Project, the application of genetic engineering-led biotechnology in the field of sports issues has received extensive attention. Sports genes are related to the determination of human sports ability. Genes, 70% of athletic ability is influenced by genetic factors. [0003] Scientists call those genes that determine human athletic ability sports genes. So far, researchers have discovered a number of important DNA polymorphism sites that are related to the excellent athletic ability of athletes in specific types of sports. Currently, the...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/16C12Q2600/172C12Q2531/113C12Q2537/143C12Q2533/101C12Q2565/627
Inventor 丁峰刘万飞
Owner 沈阳哥瑞科技有限公司
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