Super-resolution device based on annular optical tweezers and dark field microscopy and resolution method thereof
A ring light and super-resolution technology, applied in the field of optical microscopy, can solve the problems of high price, complicated operation, huge equipment, etc., and achieve the effect of improving contrast and resolution.
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[0027] Embodiment 1: as Figure 1-4 As shown, this embodiment uses living yeast cells for illustration, but this should not limit the protection scope of the present invention. For example, silicon beads, chromium particles, polystyrene pellets, etc. are all within the scope of protection.
[0028] Compared with traditional optical tweezers, ring optical tweezers have the following advantages: first, the laser power required for trapping can be reduced, and at the same time, multiple microscopic particles can be controlled; The region can capture particles with a relative refractive index greater than 1 and particles with a relative refractive index smaller than 1; third, the central dark region will weaken the light scattering force, and the optical thermal damage to the trapped particles will be very large under the same power condition. Small enough not to destroy the activity of biological cells.
[0029] The discovery of dark-field microscopic imaging has solved the pro...
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