Super-resolution device based on annular optical tweezers and dark field microscopy and resolution method thereof

A ring light and super-resolution technology, applied in the field of optical microscopy, can solve the problems of high price, complicated operation, huge equipment, etc., and achieve the effect of improving contrast and resolution.

Pending Publication Date: 2019-10-22
KUNMING UNIV OF SCI & TECH
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Problems solved by technology

Although the resolution of microscopes such as scanning electron microscopes developed in recent decades can reach the micronano level, there are also disadvantages such as bulky equipment, expensive prices, and complicated operations.

Method used

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  • Super-resolution device based on annular optical tweezers and dark field microscopy and resolution method thereof
  • Super-resolution device based on annular optical tweezers and dark field microscopy and resolution method thereof
  • Super-resolution device based on annular optical tweezers and dark field microscopy and resolution method thereof

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Embodiment 1

[0027] Embodiment 1: as Figure 1-4 As shown, this embodiment uses living yeast cells for illustration, but this should not limit the protection scope of the present invention. For example, silicon beads, chromium particles, polystyrene pellets, etc. are all within the scope of protection.

[0028] Compared with traditional optical tweezers, ring optical tweezers have the following advantages: first, the laser power required for trapping can be reduced, and at the same time, multiple microscopic particles can be controlled; The region can capture particles with a relative refractive index greater than 1 and particles with a relative refractive index smaller than 1; third, the central dark region will weaken the light scattering force, and the optical thermal damage to the trapped particles will be very large under the same power condition. Small enough not to destroy the activity of biological cells.

[0029] The discovery of dark-field microscopic imaging has solved the pro...

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Abstract

The invention relates to a super-resolution device based on annular optical tweezers and dark field microscopy and a resolution method thereof, and belongs to the field of optical microscopy. A tapered mirror is configured to generate a local hollow light beam. The local hollow light beam is adjusted by a lens to have a suitable size, and then enters a dark field condensing lens to be compressed and condensed, thereby forming annular optical tweezers to trap particles in a Petri dish in the vicinity of annular light. The scattered light of the trapped particles provides an outline of the annular light, thereby visualizing the annular light under dark field microscopy. The distance between the observed particles and the dark field condensing lens is adjusted in order to gradually change thesize of the annular light to approximate a particle size. When the size of the annular light is close to the size of a single particle, the particles are stably trapped in the vicinity of the maximumlight intensity gradient, and the contrast is increased at this time and the resolution is maximized. The super-resolution device can be used in the fields of holographic super-resolution, optical capture, image enhancement and the like, and has a good application prospect.

Description

technical field [0001] The invention relates to a super-resolution device based on annular optical tweezers and dark-field microscopy and a resolution method thereof, which is a technology for realizing super-resolution of nanoscale particles and belongs to the field of optical microscopy. Background technique [0002] Introduced in 1986, optical tweezers are particularly suitable for manipulating living cells due to their ability to manipulate and capture particles without damage and contact. It occupies an increasingly important share in the fields of biology, medicine, and microprocessing. In the optical tweezers, photons and medium particles exchange momentum to generate scattering force and gradient force. The direction of scattering force is along the propagation direction of light, so that the particles move along the direction of beam propagation. Gradient force is proportional to the gradient of light intensity and points to the maximum intensity of light field. , ...

Claims

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Application Information

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IPC IPC(8): G02B21/10G02B21/32G02B21/36
CPCG02B21/10G02B21/32G02B21/361
Inventor 楼宇丽杨力李重光刘庆伟
Owner KUNMING UNIV OF SCI & TECH
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