Production method for reducing infection of cultivated species of dictyophrarubrovolvata by streptomyces spp.
A production method, the technology of Dictyophora edulis, which is applied in cultivation, plant cultivation, mushroom cultivation, etc., can solve the problems of slow growth of Dictyophora edulis, increase the probability of infection, and affect the quality of bacteria, so as to reduce the spread of pests The probability of Neurospora, shortening the time, and the effect of shortening the culture time of the strain
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Embodiment 1
[0044] ① The culture medium formula is as follows: birch wood chips 77.8%, wheat bran 15%, corn flour 3%, soybean flour 2%, sugar 1%, gypsum 1%, potassium dihydrogen phosphate 0.1% and magnesium sulfate 0.1%. Regulate the water content of the culture medium to 60%, and the appearance shows that it is moist but not dripping, and it is kneaded into a ball by hand, and water overflows but does not drip when it is seen between the fingers.
[0045] ②Cultivation bag: use a polyethylene bag with a size of 330mm×150mm×0.07mm and a thickness of 0.07mm.
[0046] ③ Medium filling: 800g per bag, insert a 180mm long inoculation rod in the center of the bag, put a collar on it, and cover the cover.
[0047] ④High-pressure steam sterilization: Put the filled cultivation bag in a sterilizing pot, and keep it under the condition of 0.11Mpa for 120 minutes after excluding the cold air.
[0048] ⑤Selection and treatment of the original seed: 500ml bottled original seed that has just overgrown ...
Embodiment 2
[0058] ① The formula of cultivar medium is as follows: walnut sawdust 74.8%, wheat bran 16%, corn flour 4%, soybean flour 3%, sugar 1%, gypsum 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.1%. Regulate the water content of culture medium to 63%, and the outward appearance is shown as moist but not dripping water, and hands kneads into a ball, and between fingers sees that water overflows but does not drip.
[0059] ② Cultivation bag: Use 330mm×150mm×0.07mm polyethylene bag with a thickness of 0.07mm.
[0060] ③ Medium filling: 850g per bag, insert a 180mm long inoculation rod in the center of the bag, put a collar on it, and cover the cover.
[0061] ④High-pressure steam sterilization: Put the filled cultivation bag in a sterilizing pot, and keep it under the condition of 0.13Mpa for 120 minutes after excluding the cold air.
[0062] ⑤Selection and treatment of the original seed: 500ml bottled original seed that has just overgrown or will soon be full of pollut...
Embodiment 3
[0072] ① The culture medium formula is as follows: 80% chestnut wood chips, 10% wheat bran, 4% corn flour, 2% soybean flour, 2% sugar, 1% gypsum, 0.5% potassium dihydrogen phosphate and 0.5% magnesium sulfate. The water content of the medium was adjusted to 55%.
[0073] ②Cultivation bag: Use 330mm×150mm×0.07mm polyethylene bag with a thickness of 0.08mm.
[0074] ③ Medium filling: 750g per bag, insert a 180mm long inoculation rod in the center of the bag, put a collar on it, and cover the cover.
[0075] ④ High-pressure steam sterilization: Put the filled cultivation bags in a sterilizing pot, remove the cold air, sterilize at normal pressure, and keep the temperature for 10 hours after reaching 100°C.
[0076] ⑤Selection and treatment of the original seed: 500ml bottled original seed that has just overgrown or will soon be full of pollution, white mycelia, strong mycelium, and no shrinkage and aging at the bottle mouth, and use 0.1% potassium permanganate solution for the o...
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