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SV40 molecular marker in NELFA (negative elongation factor complex member A) gene related to litter size of mini pigs and application

A technology of molecular markers and litter size, applied in the direction of recombinant DNA technology, DNA/RNA fragments, microbial determination/inspection, etc., can solve the problems of slow growth, slow development of improved pig breeds, pig breed improvement and breeding start Late and other problems, to achieve the effect of easy operation

Pending Publication Date: 2019-10-25
GUIZHOU UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Many European countries have carried out breeding work on growth rate, reproductive rate, lean meat rate, etc. since the last century, so many European domestic pigs have been better improved in this series of important economic traits (Falkenberg H et al. al, 2008), compared with developed countries in Europe, the improvement and selection of local pig breeds in my country started relatively late, because the development of improved pig breeds is slow, so Guizhou has abundant pig breed resources. These pig breeds have meat quality, resistance It has outstanding advantages in terms of disease ability, resistance to rough feeding, and adaptability, but its reproductive rate varies greatly and its growth rate is relatively slow (Zhang Yun et al., 2007).

Method used

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  • SV40 molecular marker in NELFA (negative elongation factor complex member A) gene related to litter size of mini pigs and application
  • SV40 molecular marker in NELFA (negative elongation factor complex member A) gene related to litter size of mini pigs and application
  • SV40 molecular marker in NELFA (negative elongation factor complex member A) gene related to litter size of mini pigs and application

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1 is the detection method of the present invention, and the steps are as follows:

[0033] 1. Genomic DNA extraction from pig ear tissue

[0034] Genomic DNA from pig ear tissue was extracted using Tiangen Spin Column Tissue Genomic DNA Extraction Kit (catalogue number: DP304), and the steps were as follows:

[0035] (1) Mince an appropriate amount of pig ear tissue and place it in a 1.5mL centrifuge tube, add an appropriate amount of buffer GA (50mg ear tissue with 200μL buffer GA), shake thoroughly to suspend the tissue fragments;

[0036] (2) Add 4 μL RNaseA (100 mg / mL), shake for 15 s, and place at room temperature for 5 min to remove RNA in the tissue;

[0037] (3) Add 20 μL of Proteinase K solution, mix well and place in a 56°C water bath for 1 to 3 hours until the tissue is completely dissolved, then briefly centrifuge to remove water droplets on the tube wall;

[0038] (4) Add 200 μL cell lysate GB, mix well by inverting, and bathe in 70°C water bat...

Embodiment 2

[0055] Example 2 Detection and Application of Structural Variation SV40

[0056] According to the method of Example 1, with the porcine genomic region structural variation site (chr8: 1111424-1111463) as the candidate site, SV40 was detected by PCR technology in the population (226 fragrant pigs, 106 individuals with high litter size, Structural variation in individuals with low litter size (120 pigs), blood samples from 226 Xiang pigs were collected from Zijiang County. Using the SV40 candidate region, applying the present invention to establish the detection technology to analyze the distribution frequency of the structural variation SV40 in the population, the results show that the genotyping results show that there are 3 genotypes in the high-yield population and low-yield population, and all of them are mainly type II Genotype (Table 1).

[0057] Statistics of the litter size of the first three litters of Xiang pigs and calculation of the average (Table 2), found that am...

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Abstract

The invention discloses an SV40 molecular marker in an NELFA (negative elongation factor complex member A) gene related to the litter size of mini pigs and application. The SV40 molecular marker is characterized in that SV4 structural variation deletes 40bp as compared with a pig reference genome (Sscrofa 11.1), and is located in an intron 1 of the NELFA gene, wherein the interval is chr8:50916410-50916886. Correlation analysis of reproductive traits and structures of the mini pigs shows that SV40 deletion or normality has remarkable influences on the litter size of the min pigs; according toanalysis of the size of the first three litters of the mini pigs, the litter size corresponding to type II is highest, and the litter size corresponding to type DD is lowest.

Description

technical field [0001] The invention relates to the field of animal molecular biology detection, in particular to a SV40 marker related to the litter size of fragrant pigs and its application technology. Background technique [0002] Pig is a very important economic animal. Its quantitative and qualitative traits such as fecundity, disease resistance, meat quality, growth and economic traits litter size have always attracted the attention of scholars. During the domestication process, due to environmental climate, geography Differences in ecology, feeding conditions, and breeding methods have resulted in large differences in appearance and performance between domestic and foreign pig breeds. However, local pigs in my country are very distinctive (Shang Yishun et al., 2017). Many European countries have carried out breeding work on growth rate, reproductive rate, lean meat rate, etc. since the last century, so many European domestic pigs have been better improved in this serie...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172C12Q2531/113C12Q2565/125
Inventor 冉雪琴牛熙王嘉福卢圣菲
Owner GUIZHOU UNIV