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Method for determining photodecomposition of drug for controlling tomato grey mould

A tomato cinerea, photolysis technology, applied in the direction of testing pharmaceutical preparations, material inspection products, etc., can solve the problems that do not involve the biological activity of the drug, cannot be enhanced or weakened, and cannot reflect the relationship between drug degradation, etc., to achieve an overall technically simple effect

Active Publication Date: 2019-10-25
GUANGXI UNIV
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current technical means have a common feature. The detection process only tracks the original molecular structure of the drug, and does not involve the biological activity of the drug on the control object. As a result, it is impossible to obtain relevant information on whether the activity of the drug degradation product on the control object is enhanced or weakened. Failure to reflect the relationship between drug degradation and efficacy
[0004] Whether the photolysis of the drug can directly affect its activity on the control object, and its activity can be displayed by affecting the growth and development of the control object. However, there is no way to use the biological activity of the drug as a drug photolysis effect so far. detection technology

Method used

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  • Method for determining photodecomposition of drug for controlling tomato grey mould
  • Method for determining photodecomposition of drug for controlling tomato grey mould

Examples

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Embodiment 1

[0035] Apply the assay method of a kind of tomato cinerea control drug photolysis of the present invention, in common laboratory light conditions, use Botrytis cinerea bacterial strain Bc-5 as material, measure the photolysis of fungicide iprodione, as follows Steps to implement:

[0036] 1. Equipped with agar medium: Potato dextrose agar (PDA) suitable for the growth of Botrytis cinerea was used as the test medium, according to the proportion: 200g of potatoes, 20g of glucose, 20g of agar, and 900mL of water were prepared and routinely sterilized at high temperature for use.

[0037] 2. Preparation of drug plates: It is known that the mycelium growth of Botrytis cinerea strain Bc-5 is sensitive to iprodione, and the medium concentration EC 50 1 μg / mL. Under sterile conditions, fully mix the iprodione liquid with the PDA medium prepared in step 1 to prepare a drug-containing medium with a ratio of 1 μg / mL of iprodione, and use an ordinary petri dish to pour the plate to obtai...

Embodiment 2

[0045] The method for measuring the photolysis of the tomato gray mold prevention and control drug according to the present invention is used to measure the photolysis of the fungicide iprodione under light conditions in a greenhouse with Botrytis cinerea strain Bc-5 as a material. According to step 1 to step 6 of embodiment 1, the difference is that the light treatment condition of step 4 is not the light condition that the natural light intensity is 1000Lux at noon by the ordinary indoor window, but the light condition that the natural light intensity is 11000Lux at noon in the greenhouse , The results of measuring the photolysis of iprodione are shown in Table 2.

[0046] Table 2. Changes in iprodione inhibitory activity before and after light treatment of Example 2

[0047]

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Abstract

The invention discloses a method for determining photodecomposition of a drug for controlling tomato grey mould. According to the method, a fungicide drug is mixed with an agar medium to prepare a culture plate, photodecomposition of the drug is caused by illumination treatment, a change in activity of a phototreatment product is caused by the photodecomposition of the drug, and the photodecomposition of the drug is displayed through testing the reaction of botrytis cirerea on the phototreatment product. The method comprises the implementation steps of (1) preparing the agar medium; (2) preparing a drug plate; (3) carrying out drug activity detection before phototreatment; (4) carrying out phototreatment; (5) carrying out drug activity detection after phototreatment; and (6) obtaining a photodecomposition result. The method has the advantages that (1) the overall technology is simple and operation by using high-precision instrument and equipment or a complex serological technique is not needed; (2) the relationship between photodecomposition of the drug and the pesticide effect can be reflected; and (3) information of a change in drug activity caused by non-photodecomposition can be obtained.

Description

technical field [0001] The invention relates to a detection technology for the photolysis of a fungicide, in particular to a method for measuring the photolysis of a tomato gray mold control drug. Background technique [0002] Botrytis cinerea is an important disease of tomato, a staple vegetable. The prevalence of the disease can bring heavy losses to tomato production. Currently, the control of this disease mainly depends on the control of pesticides. Once the control drugs for pesticide control are applied in the field, they usually degrade naturally, and the photodecomposition effect caused by light is the most prominent. Therefore, photolysis is closely related to the control effect of drugs and drug residues. Obviously, understanding the law of photolysis of drugs is of great significance to give full play to the preventive effect of drugs and reduce drug residues. [0003] At present, the detection technology of photolysis of drugs mainly uses physical and chemical ...

Claims

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Application Information

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IPC IPC(8): G01N33/15
CPCG01N33/15
Inventor 张君成吴海燕王忠文
Owner GUANGXI UNIV
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