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Method and system for discovering utilization of ceRNA mechanism by long non-coding RNA molecular markers through multi-data platform and application

A technology of long-chain non-coding and molecular markers, applied in bioinformatics, instrumentation, genomics, etc., can solve problems that cannot be completed independently

Pending Publication Date: 2019-11-05
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to solve the problem of not being able to independently complete the bioclinical informatics analysis of disease-related LncRNA and its regulation of downstream genes as ceRNA

Method used

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  • Method and system for discovering utilization of ceRNA mechanism by long non-coding RNA molecular markers through multi-data platform and application
  • Method and system for discovering utilization of ceRNA mechanism by long non-coding RNA molecular markers through multi-data platform and application
  • Method and system for discovering utilization of ceRNA mechanism by long non-coding RNA molecular markers through multi-data platform and application

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Embodiment

[0105] Firstly, the original data of the data platform is processed, based on the results of LncRNA differential analysis, combined with the results of clinical characteristics analysis to screen suitable LncRNAs, miRNA predictions can be made based on their sequence characteristics and ceRNA mechanism characteristics, and the target genes of miRNAs can be further predicted, and finally Screen out target genes associated with disease. On the basis of the above analysis, a series of statistical and visual analysis are carried out.

[0106] 1. The list of LncRNA expression values ​​is shown in Table 1

[0107] Analysis platform: R platform

[0108] Analysis software: limma

[0109]

[0110] 2. The list of LncRNA expression values ​​is shown in Table 2

[0111] Analysis platform: R platform

[0112] Analysis software: limma

[0113]

[0114] 3. The list of differentially expressed LncRNA expression value files is shown in Table 3

[0115] Analysis platform: R platform...

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Abstract

The invention discloses a method and system for discovering the utilization of a ceRNA mechanism by long non-coding RNA molecular markers through a multi-data platform and application. LncRNA expression data is analyzed by means of various bioinformatic analysis methods based on rectal carcinoma LncRNA sequencing data of TANRIC public data resources, LncRNAs related to rectal carcinoma are identified, the LncRNA-related ceRNA mechanism is studied, and finally, an LncRNA-miRNA-mRNA-disease interaction map is formed. According to the method and the system, abundant and diverse bioinformatic methods are adopted, the data resources are integrated, a complete analysis process is established, it can be effectively discovered that the LncRNA molecules related to rectal carcinoma exert their biological effects through the ceRNA mechanism, in the process, high-throughput data of a public database is utilized, the scientific research cost is reduced, and the analysis efficiency is improved. By means of the method and the system, multiple risk LncRNAs can be discovered, great significance is provided for biological targeted treatment of complex diseases, gene function prediction, ceRNA mechanism exploration and gene biological effect and prognosis evaluation, and the method and the system can be widely applied to biological research work and can also be suitable for clinical related application.

Description

technical field [0001] The present invention relates to a biostatistics method and system and its application, in particular to a method and system for discovering long-chain non-coding RNA molecular markers using a multi-data platform using a ceRNA mechanism and its application. Background technique [0002] Bioinformatics is an interdisciplinary subject, covering many disciplines such as life sciences, mathematics, statistics, physics, chemistry, informatics and computer technology. It is one of the major frontier fields of life science and natural science today, and it will also be one of the core fields of natural science in the 21st century. The research tools of bioinformatics mainly include computers, software and the Internet, and the research objects mainly include nucleic acid sequences and protein sequences. Bioinformatics aims to store, edit, process, disseminate and analyze a large amount of original data, summarize the changes in the data, reveal the biologica...

Claims

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Application Information

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IPC IPC(8): G16B20/00G16B25/00G16B50/30
CPCG16B20/00G16B25/00G16B50/30
Inventor 陈瑞恽俊李晓波高娜
Owner SOUTHEAST UNIV