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Preparation method for instant sodium hyaluronate

A sodium hyaluronate and instant technology, which is applied in the field of preparation of instant sodium hyaluronate, can solve the problems of sodium hyaluronate damage, product water holding capacity, and product quality, and achieve the effect of solving damage

Active Publication Date: 2019-11-08
山东百阜福瑞达制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For example, the national invention patent with the patent number of 201811492399.4 discloses a preparation method of instant sodium hyaluronate, taking sodium hyaluronate powder and dissolving it in water to form sodium hyaluronate solution; then adding sodium chloride; precipitating sodium hyaluronate by ethanol ; then emulsify by shearing until standing for 30 minutes without stratification; then carry out solid-liquid separation, then dehydrate the solid and dry; then successively pulverize through a hammer mill and an ultrafine pulverizer to obtain instant sodium hyaluronate powder, However, this method is to carry out ethanol precipitation and purification of sodium hyaluronate, after drying the sodium hyaluronate to purify the solid, obtain sodium hyaluronate powder with a smaller particle size by means of mechanical crushing, and adopt mechanical crushing, although it can reach the particle size Sodium hyaluronate powder with a small diameter, but the instant solubility is not good, and due to the destruction of the structure of sodium hyaluronate by mechanical pulverization, it has a great impact on the water holding capacity of the product, which seriously affects the quality of the product

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  • Preparation method for instant sodium hyaluronate
  • Preparation method for instant sodium hyaluronate
  • Preparation method for instant sodium hyaluronate

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specific Embodiment approach

[0030] The specific embodiment of the present invention: the preparation method of instant sodium hyaluronate, it is characterized in that described preparation method comprises the steps:

[0031] (1) adding low-molecular-weight sodium hyaluronate or chitosan to a sodium hyaluronate solution with a mass fraction of 0.5%;

[0032] (2) Then slowly add an organic solvent to configure a mixed solution containing an organic solvent with a preset concentration;

[0033] (3) The mixed solution was freeze-dried in a low-pressure vacuum environment at -60°C to obtain the product.

[0034] Further, the molecular weight of sodium hyaluronate in the sodium hyaluronate solution is 1.20-1.4 million Da; the molecular weight of the low molecular weight sodium hyaluronate is less than 200,000 Da, and the N-deacetylation degree of the chitosan is More than 95%; the addition of the low molecular weight sodium hyaluronate or chitosan is 5% of the total mass of the sodium hyaluronate solution wi...

Embodiment 1

[0046] Example 1: A sodium hyaluronate solution with a mass fraction of 0.5% and a molecular weight of 1.2-1.4 million Da prepared by the configuration method is miscible with low-molecular-weight sodium hyaluronate, and the method for preparing instant sodium hyaluronate by vacuum freeze-drying Proceed as follows:

[0047] (1) Add low-molecular-weight sodium hyaluronate with a molecular weight of 100,000 Da to a sodium hyaluronate solution with a molecular weight of 0.5% of 0.5%; the amount of low-molecular-weight sodium hyaluronate is the mass fraction 5% of the total mass of the 0.5% sodium hyaluronate solution; the 0.5% sodium hyaluronate solution is prepared by slowly adding 0.5% sodium hyaluronate by water weight into purified water and dissolving it completely;

[0048] (2) Then slowly add absolute ethanol to form a 2% ethanol mixed solution;

[0049] (3) Freeze-dry the ethanol mixed solution in a low-pressure vacuum environment at -60°C to obtain the product.

Embodiment 2

[0050] Example 2: The sodium hyaluronate solution with a molecular weight of 0.5% and a molecular weight of 1.20-1.4 million Da prepared by the configuration method is miscible with chitosan with a degree of N-deacetylation of more than 95%, and is prepared by vacuum freeze-drying. The method steps of sodium hyaluronate are as follows:

[0051] (1) Adding the N-deacetylation degree of chitosan to the sodium hyaluronate solution with a molecular weight of 0.5% for 1.2-1.4 million Da is more than 95%; the N-deacetylation degree is more than 95% for chitosan The amount of sugar added is 5% of the total mass of the sodium hyaluronate solution; the sodium hyaluronate solution with a mass fraction of 0.5% is prepared by slowly adding 0.5% sodium hyaluronate by water weight into purified water and dissolving it completely;

[0052] (2) Then slowly add absolute ethanol to form a 2% ethanol mixed solution;

[0053] (3) Freeze-dry the ethanol mixed solution in a low-pressure vacuum env...

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Abstract

The invention discloses a preparation method for instant sodium hyaluronate. The preparation method is characterized in that: after a sodium hyaluronate solution is structured with low molecular sodium hyaluronate or chitosan, the two factors produce a synergistic effect by addition of an ethanol solution, the instant sodium hyaluronate is obtained by freeze-drying in a low pressure vacuum environment, and therefore, the damage caused by mechanical pulverization to a structure of sodium hyaluronate is effectively solved, and the superior instant-solubility effect is achieved.

Description

Technical field: [0001] The invention belongs to the technical field of macromolecule preparation, and in particular relates to a preparation method of instant sodium hyaluronate. Background technique: [0002] Sodium hyaluronate (Hyaluronic acid, HA), also known as hyaluronic acid, is composed of (1-3)-2-N-acetylamino-2-deoxy-D-glucose-(1-4)-O-β-D- An acidic linear polymucopolysaccharide formed by the repeated arrangement of disaccharides of glucuronic acid, which has strong hydrophilicity and very good moisturizing properties, and is widely present in the cytoplasm of animal tissue cells and the membrane of some bacteria. It is used in medicine, cosmetics and food, and is also recognized as the most ideal natural moisturizing factor by the international cosmetics industry. [0003] With the continuous improvement of life, the quality of products required in life is on the rise, and HA is beyond doubt in terms of moisturizing. In high-quality, high-paced life, people have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCC08B37/0072
Inventor 刘磊杜帅康传利李庆刘蔷张美霞汤丽伟高瑞史劲松龚劲松
Owner 山东百阜福瑞达制药有限公司