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Rapid propagation method of Taishan varbuschianum

A fast and lily technology, applied in the field of plant biology, can solve the problems of limited artificial breeding, self-flowering infertility, slow natural reproduction speed, etc., and achieve the effect of filling the technical gap

Inactive Publication Date: 2019-11-12
TAISHAN RES INST OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the above deficiencies, and provides the rapid propagation method of Taishan Lily spot. The test material of the present invention comes from the wild Lilium spot growing under the forest on the sunny slope of Mount Tai at an altitude of about 800m. Without destroying wild germplasm resources, quickly obtain a large number of complete small plants of Lilium variegata, form a technical system for rapid propagation of tissue culture seedlings, and effectively solve the scarcity of wild resources of Lily variegata in Mount Tai, which is endangered, slow natural reproduction speed, and autoflowering infertility , the low survival rate of transplanting and the limitation of artificial breeding are of great significance to the ex situ protection, ex situ breeding, effective utilization and lily breeding of Taishan wild lily and similar types + endangered wild plant species that urgently need to be protected. It can not only significantly improve the current situation that there is no artificial introduction of Taishan lily, but also fill the technical gap in the artificial breeding research of Taishan lily

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0031] Take a single wild lily lily plant under the forest on the sunny slope of Mount Tai at an altitude of about 800m as the test plant. At the initial stage of flowering, collect its complete raceme. The inflorescence has 1-3 unopened flower buds, the smallest flower bud is green, and the length is 3-5cm. The tip of the largest bud is about 1 / 3-1 / 2 red, the length is more than 5cm, and the buds are not open; the height of the inflorescence is 15-30cm, and the isolated inflorescence of wild Lilium variegata is used as the test material for artificial cultivation.

[0032] The isolated inflorescences are cultivated in a closed environment in the cultivation room by means of hydroponics, and there are no flowers of the same type in the cultivation room;

[0033] Taishan natural well water is used for hydroponics, pH is about 6.7, and the temperature is natural water temperature, about 20-25°C; the average daily temperature for cultivation is 18-26°C, and the average daily light...

Embodiment approach 2

[0044] Take a single wild Lilium variegata plant under the forest on the sunny slope of Mount Tai at an altitude of about 800m as the test plant, cut the 15-22d old capsule, peel off the attached perianth, wash the surface with running water, and then carry out aseptic disinfection. First disinfect with 70% alcohol for 20-30s, then disinfect with 0.1% mercuric chloride solution for 8-11min, then rinse with sterile water 3-5 times, absorb excess water on the surface, and use the sterile ovary as the test material.

[0045] Cut the sterile ovary along the dorsal suture to reveal the yellow-white immature embryo. The immature embryos were sowed on the immature embryo culture medium with tweezers, and after 20-30 days of dark culture, the immature immature embryos began to differentiate into green embryogenic callus. Continue to culture this type of embryogenic callus on the same medium, subculture once every 20-30 days, after 2-3 subcultures, the volume of embryogenic callus will...

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Abstract

The invention discloses a rapid propagation method of Taishan varbuschianum. The rapid propagation of Taishan varbuschianum is completed through steps of ovary disinfection, immature embryo culture, proliferation culture, seedling hardening and transplanting, and the like. The test material is from wild varbuschianum single plants growing under forests on a sunny hillside with an altitude of about800m in Taishan. Through immature embryo culture, large quantities of varbuschianum complete plantlets can be rapidly obtained without damaging wild germplasm resources, and a tissue culture seedlingrapid propagation technical system is formed, so that the problems of endangered wild resources of Taishan varbuschianum, slow natural propagation speed, self-flowering infertility, low transplantingsurvival rate, limited artificial propagation and the like are effectively solved; and the method is of great significance to ex-situ conservation, ex-situ propagation, effective utilization and lilybreeding of Taishan wild varbuschianum and similar types+endangered wild plant species in urgent need of protection, which can not only significantly improve the current situation of no artificial introduction of Taishan varbuschianum, but also fill in the technical blank of artificial propagation research of Taishan varbuschianum.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a rapid propagation method of Taishan lily. Background technique [0002] spotted lily ( Lilium concolor var. pulchellum ) is the genus Liliaceae (Liliaceae) ( Lilium ) Plant Wodan ( Lilium concolor Salisb. ), mainly produced in Hebei, Shandong, Shanxi, Inner Mongolia, Liaoning, Heilongjiang and Jilin. It grows in sunny slope meadows and forest wetlands, at an altitude of 600-2170 meters. North Korea and the Soviet Union are also distributed. Its bulbs contain starch, which can be used for food or wine making, and can also be used as medicine, which has the effect of nourishing, strengthening and relieving cough. The flowers contain aromatic oils and can be used as spices. Due to the scarcity of wild provenances of Lilium variegata, low self-fertilization rate, and slow natural reproduction speed, it is rarely distributed in the Taishan area; and artificial transplantat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 黄艳艳
Owner TAISHAN RES INST OF FORESTRY