Method for evaluating toxicity of solid particles by use of luminous bacteria
A technology of luminescent bacteria and solid particles, which is applied in the analysis of individual particles, material analysis by optical means, and analysis by chemical reaction of materials, etc., can solve the problems of inability to assess the true toxicity of solid substances and inaccurate experimental results. Achieve the effect of low cost, accurate and reliable results, and convenient operation
Inactive Publication Date: 2019-11-12
NANJING COLLEGE OF INFORMATION TECH
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This invention patent application has constructed a convenient method for soil comprehensive toxicity detection. However, the toxicity of the luminescent bacteria is based on the extraction solution. This method actually reflects the toxicity of some components in the solid matter that can be turned to the extraction agent, and does not Detect the toxicity of components that are combined with solid particles and have not been transferred to the extractant, and cannot evaluate the true toxicity of solid substances, making the experimental results inaccurate
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[0041] Example-solid phase toxicity test
[0042] A method for evaluating the toxicity of solid particles using luminescent bacteria, including the following steps:
[0043] Step 1. Choose 4 different lakes, collect the 0-10cm surface sediment with grab sampler, remove impurities through a 1.8mm mesh sieve, air-dry, and crush until the particle size is less than 2mm to obtain solid particles to be measured, which are respectively recorded as sediments 1. Precipitate 2, Precipitate 3, and Precipitate 4; mix 3 g of the solid particles to be tested with 30 mL of solvent (3% (w / v) sodium chloride solution), and stir for 10 minutes on a magnetic stirrer to make it fully mixed Use this as the mother liquor (100% solution), and then use 3% sodium chloride solution as the solvent to prepare 50%, 25%, and 12.5% concentration test samples by 1:2 serial dilution method, and use 3% chlorination The sodium solution is a blank sample.
[0044] Step 2. Add the Vibrio fischeri bacteria solution ...
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The invention discloses a method for evaluating toxicity of solid particles by use of luminous bacteria. The method comprises steps as follows: collecting to-be-measured solid sediments, pretreating the to-be-measured solid sediments, and then, diluting the to-be-measured solid sediments with a solvent to obtain experimental samples with different concentrations, wherein the solvent used during dilution is taken as a blank sample; adding a luminous bacteria solution to each experimental sample and the blank sample, exposing the mixture for 15-20 min, filtering the mixture with a filtering membrane to obtain filtrate, taking the same volume of filtrate, putting the filtrate in a rapid water quality toxicity detector, and measuring a luminous value of the luminous bacteria after 5 min; calculating bacteria loss rates of each experimental sample and the blank sample on the basis of a flow cytometry method; calculating the luminous value of the luminous bacteria after correction of each experimental sample and the blank sample according to the bacteria loss rates; and calculating a corrected luminous value inhibiting rate according to the corrected luminous value of the luminous bacteria to evaluate the toxicity of the solid particles. By means of method, the toxicity of the solid particles can be really evaluated, and a result is accurate and reliable.
Description
Technical field [0001] The invention belongs to the technical field of environmental detection, and specifically relates to a method for evaluating the toxicity of solid particles by using luminescent bacteria. Background technique [0002] Luminescent bacteria are used for environmental pollution detection. The traditional luminescent bacteria toxicity test exposes the luminescent bacteria to the substance to be tested, and then detects the change in the luminous intensity of the luminescent bacteria to characterize the toxicity of the detected substance. [0003] The current methods for detecting the toxicity of luminescent bacteria on solid substances usually first prepare an extract, that is, the filtrate after mixing the solid substance and the extractant, and then use the extract to perform a luminescent bacteria toxicity test. For example, the invention patent applies for a comprehensive soil toxicity detection method based on the luminescent bacteria method (application num...
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Login to View More IPC IPC(8): G01N15/14G01N21/76G01N1/34G01N1/38
CPCG01N1/34G01N1/38G01N15/14G01N21/763
Inventor 袁楠楠鲍安平
Owner NANJING COLLEGE OF INFORMATION TECH